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Protein

Splicing factor 3A subunit 2

Gene

Sf3a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 8431Matrin-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 2
Gene namesi
Name:Sf3a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1308627. Sf3a2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: Ensembl
  • nuclear speck Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Splicing factor 3A subunit 2PRO_0000326552Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei10 – 101N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ6AXT8.
PRIDEiQ6AXT8.

Expressioni

Gene expression databases

GenevisibleiQ6AXT8. RN.

Interactioni

Subunit structurei

Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex (By similarity). Interacts with HTATSF1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026202.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi217 – 469253Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the SF3A2 family.Curated
Contains 1 matrin-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 8431Matrin-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0227. Eukaryota.
COG5246. LUCA.
GeneTreeiENSGT00720000108823.
HOGENOMiHOG000154298.
HOVERGENiHBG060566.
InParanoidiQ6AXT8.
KOiK12826.
OMAiGRPGYKX.
OrthoDBiEOG7034J2.
TreeFamiTF314370.

Family and domain databases

InterProiIPR031781. SF3A2_dom.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF16835. SF3A2. 1 hit.
[Graphical view]
SMARTiSM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AXT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH
60 70 80 90 100
LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKEA KEAPAQPAPE
110 120 130 140 150
KVKVEVKKFV KIGRPGYKVT KQRDTEMGQQ SLLFQIDYPE IAEGVMPRHR
160 170 180 190 200
FMSAYEQRIE PPDRRWQYLL MAAEPYETIA FKVPSREIDK AEGKFWTHWN
210 220 230 240 250
RETKQFFLQF HFKMEKPPAP PSLPAGPPGV KRPPPPLMNG LPPRPPLPDA
260 270 280 290 300
LPPPPPGGLP LPPMPPTGPA PSGPPGPPQM PPPAPGVHPP APVVHPPTSG
310 320 330 340 350
VHPPAPGVHP PAPVVHPPTS GVHPPAPGVH PPAPGVHPPA PGVHPPAPGV
360 370 380 390 400
HPPAPGVHPP APGVHPPAPG VHPPAPGVHP PPSAGVHPQA PGVHPPAPAV
410 420 430 440 450
HPQAPGVHPP APGIHPQAPG VHPQPPPGVH PAAPGVHPQP PGVHPTPMPP
460 470
MLRPPLPSDG PGNMPPPPPG N
Length:471
Mass (Da):49,890
Last modified:September 13, 2004 - v1
Checksum:i8E00B0E89721A5C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079320 mRNA. Translation: AAH79320.1.
RefSeqiNP_001011986.1. NM_001011986.1.
XP_006240947.1. XM_006240885.2.
XP_006240948.1. XM_006240886.2.
UniGeneiRn.102569.

Genome annotation databases

EnsembliENSRNOT00000026202; ENSRNOP00000026202; ENSRNOG00000019349.
GeneIDi299620.
KEGGirno:299620.
UCSCiRGD:1308627. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079320 mRNA. Translation: AAH79320.1.
RefSeqiNP_001011986.1. NM_001011986.1.
XP_006240947.1. XM_006240885.2.
XP_006240948.1. XM_006240886.2.
UniGeneiRn.102569.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026202.

Proteomic databases

PaxDbiQ6AXT8.
PRIDEiQ6AXT8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026202; ENSRNOP00000026202; ENSRNOG00000019349.
GeneIDi299620.
KEGGirno:299620.
UCSCiRGD:1308627. rat.

Organism-specific databases

CTDi8175.
RGDi1308627. Sf3a2.

Phylogenomic databases

eggNOGiKOG0227. Eukaryota.
COG5246. LUCA.
GeneTreeiENSGT00720000108823.
HOGENOMiHOG000154298.
HOVERGENiHBG060566.
InParanoidiQ6AXT8.
KOiK12826.
OMAiGRPGYKX.
OrthoDBiEOG7034J2.
TreeFamiTF314370.

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

NextBioi645532.
PROiQ6AXT8.

Gene expression databases

GenevisibleiQ6AXT8. RN.

Family and domain databases

InterProiIPR031781. SF3A2_dom.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF16835. SF3A2. 1 hit.
[Graphical view]
SMARTiSM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiSF3A2_RAT
AccessioniPrimary (citable) accession number: Q6AXT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 13, 2004
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.