Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitochondrial sodium/calcium exchanger protein

Gene

Slc8b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion, by mediating the exchange of 3 sodium ions per 1 calcium ion. Plays a central role in mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion: calcium efflux is essential for mitochondrial function and cell survival, notably in cardiomyocytes (By similarity). Regulates rates of glucose-dependent insulin secretion in pancreatic beta-cells during the first phase of insulin secretion: acts by mediating efflux of calcium from mitochondrion, thereby affecting cytoplasmic calcium responses. Required for store-operated Ca2+ entry (SOCE) and Ca2+ release-activated Ca2+ (CRAC) channel regulation: sodium transport by SLC8B1 leads to promote calcium-shuttling that modulates mitochondrial redox status, thereby regulating SOCE activity (By similarity). Involved in B-lymphocyte chemotaxis (By similarity). Able to transport Ca2+ in exchange of either Li+ or Na+, explaining how Li+ catalyzes Ca2+ exchange. In contrast to other members of the family its function is independent of K+ (By similarity).By similarity

Enzyme regulationi

Inhibited by the sodium/calcium exchanger inhibitor CGP-37157. Strongly inhibited by zinc.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAntiport, Calcium transport, Ion transport, Sensory transduction, Sodium transport, Transport
LigandCalcium, Lithium, Sodium

Enzyme and pathway databases

ReactomeiR-RNO-425561. Sodium/Calcium exchangers.
R-RNO-8949215. Mitochondrial calcium ion transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial sodium/calcium exchanger proteinCurated
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 61 Publication
Sodium/calcium exchanger protein, mitochondrial1 Publication
Sodium/potassium/calcium exchanger 61 Publication
Solute carrier family 24 member 6Imported
Solute carrier family 8 member B1Imported
Gene namesi
Name:Slc8b1Imported
Synonyms:Nckx61 Publication, Nclx1 Publication, Slc24a6Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1565818. Slc8b1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 95ExtracellularSequence analysisAdd BLAST69
Transmembranei96 – 116Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini117 – 140CytoplasmicSequence analysisAdd BLAST24
Transmembranei141 – 161Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini162 – 168ExtracellularSequence analysis7
Transmembranei169 – 189Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini190 – 205CytoplasmicSequence analysisAdd BLAST16
Transmembranei206 – 226Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini227ExtracellularSequence analysis1
Transmembranei228 – 247Helical; Name=5Sequence analysisAdd BLAST20
Topological domaini248 – 325CytoplasmicSequence analysisAdd BLAST78
Transmembranei326 – 346Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini347 – 360ExtracellularSequence analysisAdd BLAST14
Transmembranei361 – 381Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini382 – 383CytoplasmicSequence analysis2
Transmembranei384 – 404Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini405 – 416ExtracellularSequence analysisAdd BLAST12
Transmembranei417 – 437Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini438 – 445CytoplasmicSequence analysis8
Transmembranei446 – 466Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini467 – 491ExtracellularSequence analysisAdd BLAST25
Transmembranei492 – 512Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini513 – 525CytoplasmicSequence analysisAdd BLAST13
Transmembranei526 – 546Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini547 – 559ExtracellularSequence analysisAdd BLAST13
Transmembranei560 – 580Helical; Name=13Sequence analysisAdd BLAST21
Topological domaini581 – 585CytoplasmicSequence analysis5

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000004575827 – 585Mitochondrial sodium/calcium exchanger proteinAdd BLAST559

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei258Phosphoserine; by PKABy similarity1

Post-translational modificationi

Phosphorylation at Ser-258 by PKA prevents calcium overload.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ6AXS0.
PRIDEiQ6AXS0.

Expressioni

Tissue specificityi

Widely expressed. Present at higher level in pancreas, stomach, skeletal muscle and skin (at protein level). Ubiquitously expressed.2 Publications

Gene expression databases

BgeeiENSRNOG00000001383.
GenevisibleiQ6AXS0. RN.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: RGD
  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034026.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2399. Eukaryota.
COG0530. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000113743.
HOVERGENiHBG082009.
InParanoidiQ6AXS0.
KOiK13754.
OMAiAHISPQF.
OrthoDBiEOG091G05GE.
PhylomeDBiQ6AXS0.
TreeFamiTF323444.

Family and domain databases

InterProiView protein in InterPro
IPR004837. NaCa_Exmemb.
PfamiView protein in Pfam
PF01699. Na_Ca_ex. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AXS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRWLDPLW APGFLCVALI LETASGAGDL STKAHGHIQF SARGVNQTAM
60 70 80 90 100
ADCRAVCSLN TSDRCDFVKR NPDCHSEGGY LDYLKGIFCY FPPNLLPLAI
110 120 130 140 150
TLYVFWLLYL FLILGVTAAK FFCPNLSAIS TSLKLSHNVA GVTFLAFGNG
160 170 180 190 200
APDIFSALVA FSDPRTAGLA IGALFGAGVL VTTVVAGGIT ILRPFMAASR
210 220 230 240 250
PFLRDITFYM VAVFLTFTAL YLGRITLVWA LGYLGLYVFY VVTVIICTWV
260 270 280 290 300
YQRQRSRSLV HSISETPELL TDSEEDQMSS NTNSYDYGEE YRPLLLGEET
310 320 330 340 350
TGQILLQALN PLDYRKWRTQ SISCKLLKVA KLPVEFLLLL TVPVVDPDKD
360 370 380 390 400
DRNWKRPLNC LQLVISPLVL VLTLQSGVYG IYEIGGLLPV WAVVVIVGTA
410 420 430 440 450
LASVTFFATS NSEPPRLHWL FAFLGFLTSA LWINAAATEV VNILRSLGVV
460 470 480 490 500
FRLSNTVLGL TLLAWGNSIG DAFSDFTLAR QGYPRMAFSA CFGGIIFNIL
510 520 530 540 550
VGVGLGCLLQ IVRSHASEVK LEPDGLLVWV LASALGLSLV FSLVSVPLQC
560 570 580
FQLSKAYGLC LLLFYICFIV VVLLTEFGVI HLKAD
Length:585
Mass (Da):63,992
Last modified:September 13, 2004 - v1
Checksum:i08BA7C6580B4F174
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079350 mRNA. Translation: AAH79350.1.
RefSeqiNP_001017488.1. NM_001017488.1.
UniGeneiRn.13225.

Genome annotation databases

EnsembliENSRNOT00000038736; ENSRNOP00000034026; ENSRNOG00000001383.
GeneIDi498185.
KEGGirno:498185.
UCSCiRGD:1565818. rat.

Similar proteinsi

Entry informationi

Entry nameiNCLX_RAT
AccessioniPrimary (citable) accession number: Q6AXS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: September 13, 2004
Last modified: November 22, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families