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Protein

Sodium/potassium/calcium exchanger 6, mitochondrial

Gene

Slc8b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion, thereby acting as a key regulator of mitochondrion calcium homeostasis. Regulates rates of glucose-dependent insulin secretion in pancreatic beta-cells during the first phase of insulin secretion: acts by mediating efflux of calcium from mitochondrion, thereby affecting cytoplasmic calcium responses. Able to transport Ca2+ in exchange of either Li+ or Na+, explaining how Li+ catalyzes Ca2+ exchange. In contrast to other members of the family its function is independent of K+ (By similarity).By similarity

GO - Molecular functioni

  • calcium:sodium antiporter activity Source: UniProtKB
  • identical protein binding Source: RGD
  • protein homodimerization activity Source: BHF-UCL

GO - Biological processi

  • glucose homeostasis Source: UniProtKB
  • mitochondrial calcium ion homeostasis Source: BHF-UCL
  • mitochondrial calcium ion transport Source: UniProtKB
  • regulation of cytosolic calcium ion concentration Source: BHF-UCL
  • regulation of insulin secretion Source: UniProtKB
  • response to stimulus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antiport, Calcium transport, Ion transport, Sensory transduction, Sodium transport, Transport

Keywords - Ligandi

Calcium, Lithium, Sodium

Enzyme and pathway databases

ReactomeiR-RNO-425561. Sodium/Calcium exchangers.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium/calcium exchanger 6, mitochondrial
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 6
Sodium/calcium exchanger protein, mitochondrial
Solute carrier family 24 member 6
Solute carrier family 8 member B1
Gene namesi
Name:Slc8b1
Synonyms:Nckx6, Nclx, Slc24a6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1565818. Slc8b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 9569ExtracellularSequence analysisAdd
BLAST
Transmembranei96 – 11621Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini117 – 14024CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 16121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini162 – 1687ExtracellularSequence analysis
Transmembranei169 – 18921Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini190 – 20516CytoplasmicSequence analysisAdd
BLAST
Transmembranei206 – 22621Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini227 – 2271ExtracellularSequence analysis
Transmembranei228 – 24720Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini248 – 32578CytoplasmicSequence analysisAdd
BLAST
Transmembranei326 – 34621Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini347 – 36014ExtracellularSequence analysisAdd
BLAST
Transmembranei361 – 38121Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini382 – 3832CytoplasmicSequence analysis
Transmembranei384 – 40421Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini405 – 41612ExtracellularSequence analysisAdd
BLAST
Transmembranei417 – 43721Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini438 – 4458CytoplasmicSequence analysis
Transmembranei446 – 46621Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini467 – 49125ExtracellularSequence analysisAdd
BLAST
Transmembranei492 – 51221Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini513 – 52513CytoplasmicSequence analysisAdd
BLAST
Transmembranei526 – 54621Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini547 – 55913ExtracellularSequence analysisAdd
BLAST
Transmembranei560 – 58021Helical; Name=13Sequence analysisAdd
BLAST
Topological domaini581 – 5855CytoplasmicSequence analysis

GO - Cellular componenti

  • cytosol Source: Ensembl
  • integral component of mitochondrial membrane Source: UniProtKB
  • mitochondrial crista Source: BHF-UCL
  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: BHF-UCL
  • sarcolemma Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626MitochondrionSequence analysisAdd
BLAST
Chaini27 – 585559Sodium/potassium/calcium exchanger 6, mitochondrialPRO_0000045758Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6AXS0.
PRIDEiQ6AXS0.

Expressioni

Tissue specificityi

Widely expressed. Present at higher level in pancreas, stomach, skeletal muscle and skin (at protein level). Ubiquitously expressed.2 Publications

Gene expression databases

GenevisibleiQ6AXS0. RN.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: RGD
  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034026.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2399. Eukaryota.
COG0530. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000113743.
HOVERGENiHBG082009.
InParanoidiQ6AXS0.
KOiK13754.
OMAiNMSCRAV.
OrthoDBiEOG7M0NRK.
PhylomeDBiQ6AXS0.
TreeFamiTF323444.

Family and domain databases

InterProiIPR004837. NaCa_Exmemb.
[Graphical view]
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AXS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRWLDPLW APGFLCVALI LETASGAGDL STKAHGHIQF SARGVNQTAM
60 70 80 90 100
ADCRAVCSLN TSDRCDFVKR NPDCHSEGGY LDYLKGIFCY FPPNLLPLAI
110 120 130 140 150
TLYVFWLLYL FLILGVTAAK FFCPNLSAIS TSLKLSHNVA GVTFLAFGNG
160 170 180 190 200
APDIFSALVA FSDPRTAGLA IGALFGAGVL VTTVVAGGIT ILRPFMAASR
210 220 230 240 250
PFLRDITFYM VAVFLTFTAL YLGRITLVWA LGYLGLYVFY VVTVIICTWV
260 270 280 290 300
YQRQRSRSLV HSISETPELL TDSEEDQMSS NTNSYDYGEE YRPLLLGEET
310 320 330 340 350
TGQILLQALN PLDYRKWRTQ SISCKLLKVA KLPVEFLLLL TVPVVDPDKD
360 370 380 390 400
DRNWKRPLNC LQLVISPLVL VLTLQSGVYG IYEIGGLLPV WAVVVIVGTA
410 420 430 440 450
LASVTFFATS NSEPPRLHWL FAFLGFLTSA LWINAAATEV VNILRSLGVV
460 470 480 490 500
FRLSNTVLGL TLLAWGNSIG DAFSDFTLAR QGYPRMAFSA CFGGIIFNIL
510 520 530 540 550
VGVGLGCLLQ IVRSHASEVK LEPDGLLVWV LASALGLSLV FSLVSVPLQC
560 570 580
FQLSKAYGLC LLLFYICFIV VVLLTEFGVI HLKAD
Length:585
Mass (Da):63,992
Last modified:September 13, 2004 - v1
Checksum:i08BA7C6580B4F174
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079350 mRNA. Translation: AAH79350.1.
RefSeqiNP_001017488.1. NM_001017488.1.
XP_008767471.1. XM_008769249.1.
UniGeneiRn.13225.

Genome annotation databases

EnsembliENSRNOT00000038736; ENSRNOP00000034026; ENSRNOG00000001383.
GeneIDi498185.
KEGGirno:498185.
UCSCiRGD:1565818. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079350 mRNA. Translation: AAH79350.1.
RefSeqiNP_001017488.1. NM_001017488.1.
XP_008767471.1. XM_008769249.1.
UniGeneiRn.13225.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034026.

Proteomic databases

PaxDbiQ6AXS0.
PRIDEiQ6AXS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000038736; ENSRNOP00000034026; ENSRNOG00000001383.
GeneIDi498185.
KEGGirno:498185.
UCSCiRGD:1565818. rat.

Organism-specific databases

CTDi80024.
RGDi1565818. Slc8b1.

Phylogenomic databases

eggNOGiKOG2399. Eukaryota.
COG0530. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000113743.
HOVERGENiHBG082009.
InParanoidiQ6AXS0.
KOiK13754.
OMAiNMSCRAV.
OrthoDBiEOG7M0NRK.
PhylomeDBiQ6AXS0.
TreeFamiTF323444.

Enzyme and pathway databases

ReactomeiR-RNO-425561. Sodium/Calcium exchangers.

Miscellaneous databases

PROiQ6AXS0.

Gene expression databases

GenevisibleiQ6AXS0. RN.

Family and domain databases

InterProiIPR004837. NaCa_Exmemb.
[Graphical view]
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  2. "Molecular cloning of a sixth member of the K+-dependent Na+/Ca2+ exchanger gene family, NCKX6."
    Cai X., Lytton J.
    J. Biol. Chem. 279:5867-5876(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. "Lithium-calcium exchange is mediated by a distinct potassium-independent sodium-calcium exchanger."
    Palty R., Ohana E., Hershfinkel M., Volokita M., Elgazar V., Beharier O., Silverman W.F., Argaman M., Sekler I.
    J. Biol. Chem. 279:25234-25240(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNCKX6_RAT
AccessioniPrimary (citable) accession number: Q6AXS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: September 13, 2004
Last modified: June 8, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.