ID   S35A3_RAT               Reviewed;         326 AA.
AC   Q6AXR5;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   24-JAN-2024, entry version 126.
DE   RecName: Full=UDP-N-acetylglucosamine transporter;
DE   AltName: Full=Golgi UDP-GlcNAc transporter;
DE   AltName: Full=Solute carrier family 35 member A3;
GN   Name=Slc35a3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Transports diphosphate-N-acetylglucosamine (UDP-GlcNAc) from
CC       the cytosol into the lumen of the Golgi apparatus, functioning as an
CC       antiporter that exchanges UDP-N-acetyl-alpha-D-glucosamine for UMP. May
CC       supply UDP-GlcNAc as substrate for Golgi-resident glycosyltransferases
CC       that generate highly branched, multiantennary complex N-glycans and
CC       keratan sulfate. However, the exact role of SLC35A3 still needs to be
CC       elucidated, it could be a member of a catalytically more efficient
CC       multiprotein complex rather than function independently as a single
CC       transporter. {ECO:0000250|UniProtKB:Q9Y2D2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-N-acetyl-alpha-D-glucosamine(in) + UMP(out) = UDP-N-
CC         acetyl-alpha-D-glucosamine(out) + UMP(in); Xref=Rhea:RHEA:72695,
CC         ChEBI:CHEBI:57705, ChEBI:CHEBI:57865;
CC         Evidence={ECO:0000250|UniProtKB:Q9Y2D2};
CC   -!- SUBUNIT: Interacts with SLC35A2; the interaction is reduced in the
CC       presence of SLC35A4. Found in a complex with SLC35A2 and SLC35A4.
CC       Interacts with MGAT4B. {ECO:0000250|UniProtKB:Q9Y2D2}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000250|UniProtKB:Q9Y2D2}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- PTM: O-Glcnacylation regulates the stability of SLC35A3 and the
CC       specific complex formation with MGAT4B. {ECO:0000250|UniProtKB:Q9Y2D2}.
CC   -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BC079371; AAH79371.1; -; mRNA.
DR   RefSeq; NP_001012082.1; NM_001012082.1.
DR   AlphaFoldDB; Q6AXR5; -.
DR   SMR; Q6AXR5; -.
DR   STRING; 10116.ENSRNOP00000074264; -.
DR   PhosphoSitePlus; Q6AXR5; -.
DR   PaxDb; 10116-ENSRNOP00000021676; -.
DR   Ensembl; ENSRNOT00000085122.2; ENSRNOP00000074264.1; ENSRNOG00000015502.8.
DR   Ensembl; ENSRNOT00055001818; ENSRNOP00055001418; ENSRNOG00055001101.
DR   Ensembl; ENSRNOT00060001324; ENSRNOP00060000589; ENSRNOG00060000992.
DR   Ensembl; ENSRNOT00065038236; ENSRNOP00065030954; ENSRNOG00065022405.
DR   GeneID; 310808; -.
DR   KEGG; rno:310808; -.
DR   UCSC; RGD:1308615; rat.
DR   AGR; RGD:1308615; -.
DR   CTD; 23443; -.
DR   RGD; 1308615; Slc35a3.
DR   eggNOG; KOG2234; Eukaryota.
DR   GeneTree; ENSGT00940000157395; -.
DR   HOGENOM; CLU_024645_1_0_1; -.
DR   InParanoid; Q6AXR5; -.
DR   OMA; VPAIMYV; -.
DR   OrthoDB; 200085at2759; -.
DR   PhylomeDB; Q6AXR5; -.
DR   TreeFam; TF315345; -.
DR   Reactome; R-RNO-727802; Transport of nucleotide sugars.
DR   PRO; PR:Q6AXR5; -.
DR   Proteomes; UP000002494; Chromosome 2.
DR   Bgee; ENSRNOG00000061832; Expressed in stomach and 18 other cell types or tissues.
DR   GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; ISO:RGD.
DR   GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR   GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; ISS:UniProtKB.
DR   InterPro; IPR007271; Nuc_sug_transpt.
DR   NCBIfam; TIGR00803; nst; 1.
DR   PANTHER; PTHR10231; NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER; 1.
DR   PANTHER; PTHR10231:SF36; UDP-N-ACETYLGLUCOSAMINE TRANSPORTER; 1.
DR   Pfam; PF04142; Nuc_sug_transp; 1.
DR   PIRSF; PIRSF005799; UDP-gal_transpt; 1.
DR   SUPFAM; SSF103481; Multidrug resistance efflux transporter EmrE; 1.
DR   Genevisible; Q6AXR5; RN.
PE   2: Evidence at transcript level;
KW   Antiport; Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..326
FT                   /note="UDP-N-acetylglucosamine transporter"
FT                   /id="PRO_0000213359"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        212..232
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        269..289
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        293..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   326 AA;  36079 MW;  4C1BB88723E59C24 CRC64;
     MSANLKYLSL GILVFQTTSL VLTMRYSRTL KEEGPRYLSS TAVVVAEFLK IMACIFLVYK
     DSKCSVRTLN RVLHDEILNK PMETLKLAIP SGIYTLQNNL LYVALSNLDA ATYQVTYQLK
     ILTTALFSVS MLGKKLGMYQ WLSLVILMAG VAFVQWPSDS QELNSKDLST GSQFVGLMAV
     LIACFSSGFA GVYFEKILKE TKQSVWIRNI QLGFFGSIFG LMGVYVYDGE LVSKNGFFQG
     YNQLTWIVVV LQALGGLVIA AVIKYADNIL KGFATSLSII LSTIISYFWL QDFVPTSVFF
     LGAILVIAAT FLYGYDPKPA GNPTKA
//