Reviewed, UniProtKB/Swiss-Prot Q6AXR4 (HEXB_RAT)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase subunit beta
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase subunit beta
    Beta-N-acetylhexosaminidase subunit beta
      Short name=Hexosaminidase subunit B

Gene names

Name:

Hexb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from direct assay. Source: RGD
Cellular component	lysosome Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	beta-N-acetylglucosaminidase activity Inferred from direct assay. Source: RGD cation binding Inferred from electronic annotation. Source: InterPro protein heterodimerization activity Inferred from direct assay. Source: RGD protein homodimerization activity Inferred from direct assay. Source: RGD sugar binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 537	514	Beta-hexosaminidase subunit beta		PRO_0000012008
Sites
	Active site	333	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	305	1	N-linked (GlcNAc...) Potential
	Disulfide bond	69 ↔ 115		By similarity
	Disulfide bond	287 ↔ 338		By similarity
	Disulfide bond	512 ↔ 529		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AXR4-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 1A9BB01F4AD2F6E4	FASTA	537	61,527
	10 20 30 40 50 60 MPGSPRRAPG LLLQALVAMV SLALVAPFGL QPALWPMPRS VQVFPRLLYI SPENFQIDNS 70 80 90 100 110 120 PNSTAGPSCS LLLEAFRRYY NYIFGFYKRH HGPAKFQDKP QLEKLLVFIN LEPQCDAFPS 130 140 150 160 170 180 MSSDESYSLL VQEPVALLKA NEVWGALRGL ETFSQLVYQD AYGTFTINES TIADSPRFPH 190 200 210 220 230 240 RGILIDTSRH YLPVKTIFKT LDAMAFNKFN VLHWHIVDDQ SFPYQSITFP ELSNKGSYSL 250 260 270 280 290 300 SHVYTPNDIH MVLEYARLRG IRVIPEFDSP GHTQSWGKGQ KNLLTPCFIQ KIRTQKVGPV 310 320 330 340 350 360 DPSLNTTYVF FDTFFKEISR VFPDQFIHLG GDEVEFECWA SNPNIQNFMK KKGFGNNFRR 370 380 390 400 410 420 LESFYIKKIL DIITSLKKSS IVWQDVFDDQ VELQPGTVVE VWKSENYLNE LAQVTASGFP 430 440 450 460 470 480 AILSAPWYLD LISYGQDWRN YYKAEPLNFE GSEKQKQLVI GGEACLWGEY VDATNLIPRL 490 500 510 520 530 WPRASAVGER LWSPRIITNL ENAYRRLAVH RCRMVSRGIA AQPLFTGYCN YENKMEK

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC079376 mRNA. Translation: AAH79376.1.
IPI	IPI00464518.
RefSeq	NP_001011946.1.
UniGene	Rn.203067
3D structure databases
SMR	Q6AXR4. Positions 33-530.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AXR4.
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Genome annotation databases
Ensembl	ENSRNOT00000048554; ENSRNOP00000044480; ENSRNOG00000025274; Rattus norvegicus. [Genome view]
GeneID	294673.
KEGG	rno:294673.
UCSC	NM_001011946. rat.
Organism-specific databases
CTD	294673.
RGD	1307607. Hexb.
Phylogenomic databases
HOVERGEN	Q6AXR4.
Enzyme and pathway databases
BRENDA	3.2.1.52. 248.
Gene expression databases
ArrayExpress	Q6AXR4.
Genevestigator	Q6AXR4.
GermOnline	ENSRNOG00000025274. Rattus norvegicus.
Family and domain databases
InterPro	IPR001540. Glyco_hydro_20. IPR015883. Glyco_hydro_20_cat-core. IPR013781. Glyco_hydro_sg_catalytic. IPR015882. HexNAc-like_b. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR22600. Glyco_hydro_20. 1 hit.
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
ProtoNet	Search...
Other Resources
NextBio	638387.

Entry information

Entry name

HEXB_RAT

Accession

Primary (citable) accession number: Q6AXR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	September 13, 2004
Last modified:	October 13, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)