Q6AXR4 (HEXB_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase subunit beta
EC=3.2.1.52

Alternative name(s):
Beta-N-acetylhexosaminidase subunit beta
Short name=Hexosaminidase subunit B
N-acetyl-beta-glucosaminidase subunit beta

Gene names

Name:

Hexb

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from direct assay PubMed 1397486. Source: RGD carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	lysosome Inferred from direct assay PubMed 8702598PubMed 9041125. Source: RGD
Molecular_function	beta-N-acetylglucosaminidase activity Inferred from direct assay PubMed 1397486. Source: RGD carbohydrate binding Inferred from direct assay PubMed 8702598. Source: RGD protein heterodimerization activity Inferred from direct assay PubMed 1397486. Source: RGD protein homodimerization activity Inferred from direct assay PubMed 1397486. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 537	514	Beta-hexosaminidase subunit beta		PRO_0000012008
Sites
	Active site	333	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	305	1	N-linked (GlcNAc...) Potential
	Disulfide bond	69 ↔ 115		By similarity
	Disulfide bond	287 ↔ 338		By similarity
	Disulfide bond	512 ↔ 529		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AXR4 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 1A9BB01F4AD2F6E4	FASTA	537	61,527
	10 20 30 40 50 60 MPGSPRRAPG LLLQALVAMV SLALVAPFGL QPALWPMPRS VQVFPRLLYI SPENFQIDNS 70 80 90 100 110 120 PNSTAGPSCS LLLEAFRRYY NYIFGFYKRH HGPAKFQDKP QLEKLLVFIN LEPQCDAFPS 130 140 150 160 170 180 MSSDESYSLL VQEPVALLKA NEVWGALRGL ETFSQLVYQD AYGTFTINES TIADSPRFPH 190 200 210 220 230 240 RGILIDTSRH YLPVKTIFKT LDAMAFNKFN VLHWHIVDDQ SFPYQSITFP ELSNKGSYSL 250 260 270 280 290 300 SHVYTPNDIH MVLEYARLRG IRVIPEFDSP GHTQSWGKGQ KNLLTPCFIQ KIRTQKVGPV 310 320 330 340 350 360 DPSLNTTYVF FDTFFKEISR VFPDQFIHLG GDEVEFECWA SNPNIQNFMK KKGFGNNFRR 370 380 390 400 410 420 LESFYIKKIL DIITSLKKSS IVWQDVFDDQ VELQPGTVVE VWKSENYLNE LAQVTASGFP 430 440 450 460 470 480 AILSAPWYLD LISYGQDWRN YYKAEPLNFE GSEKQKQLVI GGEACLWGEY VDATNLIPRL 490 500 510 520 530 WPRASAVGER LWSPRIITNL ENAYRRLAVH RCRMVSRGIA AQPLFTGYCN YENKMEK

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079376 mRNA. Translation: AAH79376.1.
IPI	IPI00464518.
RefSeq	NP_001011946.1. NM_001011946.1.
UniGene	Rn.203067.
3D structure databases
ProteinModelPortal	Q6AXR4.
SMR	Q6AXR4. Positions 32-530.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000044480.
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
PTM databases
PhosphoSite	Q6AXR4.
Proteomic databases
PaxDb	Q6AXR4.
PRIDE	Q6AXR4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	294673.
KEGG	rno:294673.
UCSC	RGD:1307607. rat.
Organism-specific databases
CTD	3074.
RGD	1307607. Hexb.
Phylogenomic databases
eggNOG	COG3525.
HOGENOM	HOG000157972.
HOVERGEN	HBG005961.
InParanoid	Q6AXR4.
KO	K12373.
OrthoDB	EOG42Z4Q7.
Enzyme and pathway databases
SABIO-RK	Q6AXR4.
Gene expression databases
ArrayExpress	Q6AXR4.
Genevestigator	Q6AXR4.
GermOnline	ENSRNOG00000025274. Rattus norvegicus.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR025705. Beta_hexosaminidase_sua/sub. IPR015883. Glyco_hydro_20_cat-core. IPR015882. Glyco_hydro_20b. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PIRSF	PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTS	PR00738. GLHYDRLASE20.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
NextBio	638387.

Entry information

Entry name

HEXB_RAT

Accession

Primary (citable) accession number: Q6AXR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	September 13, 2004
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program