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Protein

Myotubularin

Gene

Mtm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine-containing peptides. Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome. Plays a role in vacuolar formation and morphology. Regulates desmin intermediate filament assembly and architecture. Plays a role in mitochondrial morphology and positioning. Required for skeletal muscle maintenance but not for myogenesis (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O = 1-phosphatidyl-1D-myo-inositol + phosphate.By similarity
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate.By similarity

Enzyme regulationi

Allosterically activated by phosphatidylinositol 5-phosphate (PI5P).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei375Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Lipid metabolism, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Myotubularin
Alternative name(s):
Phosphatidylinositol-3,5-bisphosphate 3-phosphatase (EC:3.1.3.95By similarity)
Phosphatidylinositol-3-phosphate phosphatase (EC:3.1.3.64By similarity)
Gene namesi
Name:Mtm1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1304582. Mtm1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Cell projectionfilopodium By similarity
  • Cell projectionruffle By similarity
  • Late endosome By similarity

  • Note: Localizes as a dense cytoplasmic network. Also localizes to the plasma membrane, including plasma membrane extensions such as filopodia and ruffles. Predominantly located in the cytoplasm following interaction with MTMR12. Recruited to the late endosome following EGF stimulation (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003286571 – 602MyotubularinAdd BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei495PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6AXQ4.
PRIDEiQ6AXQ4.

PTM databases

iPTMnetiQ6AXQ4.
PhosphoSitePlusiQ6AXQ4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000002516.
GenevisibleiQ6AXQ4. RN.

Interactioni

Subunit structurei

Interacts with MTMR12; the interaction modulates MTM1 intracellular localization. Interacts with KMT2A/MLL1 (via SET domain). Interacts with DES in skeletal muscle but not in cardiac muscle (By similarity). Interacts with SPEG (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058761.

Structurei

3D structure databases

ProteinModelPortaliQ6AXQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 97GRAMAdd BLAST70
Domaini163 – 538Myotubularin phosphatasePROSITE-ProRule annotationAdd BLAST376

Domaini

The GRAM domain mediates binding to PI(3,5)P2 and, with lower affinity, to other phosphoinositides.By similarity

Sequence similaritiesi

Contains 1 GRAM domain.Curated
Contains 1 myotubularin phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4471. Eukaryota.
ENOG410XPTU. LUCA.
HOGENOMiHOG000210598.
HOVERGENiHBG000220.
InParanoidiQ6AXQ4.
KOiK01108.
OrthoDBiEOG091G04DS.
PhylomeDBiQ6AXQ4.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR004182. GRAM.
IPR010569. Myotubularin-like_Pase_dom.
IPR030564. Myotubularin_fam.
IPR011993. PH_dom-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
[Graphical view]
PANTHERiPTHR10807. PTHR10807. 1 hit.
PfamiPF02893. GRAM. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6AXQ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSASDCD AHPVERESMR KVSQDGVRQD MSKSGPRLPG ESAITDKEVI
60 70 80 90 100
YICPFSGPVK GRLYITNYRL YLRSLETDLA PILDVPLGVI SRIEKMGGVT
110 120 130 140 150
SRGENSYGLD ITCKDLRNLR FALKQEGHSR RDIFDVLTRH AFPLAYNLPL
160 170 180 190 200
FAFVNEEKFK VDGWAIYNPV EEYRRQGLPD RHWRISFVNQ RYELCDTYPA
210 220 230 240 250
LLVVPYRASD DDLRRVATFR SRNRIPVLSW IHPENRAAIM RCSQPLVGVG
260 270 280 290 300
GKRSRDDERY LDIIRETNKQ TSKLTIYDAR PGVNAVANKA TGGGYEGEDA
310 320 330 340 350
YPHAELSFLD IHNIHVMRES LRRVRDIVYP HVEEAHWLSS LESTHWLEHI
360 370 380 390 400
KLLLTGAIRV ADKVASGLSS VLVHCSDGWD RTAQLTTLAM LMLDGFYRSI
410 420 430 440 450
EGFEILVQKE WISFGHKFSS RIGHGDKNHA DADRSPIFLQ FIDCVWQMTK
460 470 480 490 500
QFPTAFEFNE CFLVAILDHL YSCRFGTFLL NCEAARERQR LAERTVSVWS
510 520 530 540 550
LINSNKDEFT NPFYARESNR VIYPVTSVRH LELWVNYYIR WNPRIRQQPH
560 570 580 590 600
PMEQRYNELL ALRDDYIKKL EELQLATPTK LTDSSTPPSG SAQIAPRMQT

HF
Length:602
Mass (Da):69,352
Last modified:April 8, 2008 - v2
Checksum:i15F32DE9EE95288B
GO
Isoform 2 (identifier: Q6AXQ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     546-546: R → RQ

Note: No experimental confirmation available.
Show »
Length:603
Mass (Da):69,480
Checksum:i8B4A2B360F93D189
GO

Sequence cautioni

The sequence AAH79400 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH91225 differs from that shown. Reason: Erroneous termination at position 553. Translated as Glu.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032751546R → RQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079400 mRNA. Translation: AAH79400.1. Different initiation.
BC091225 mRNA. Translation: AAH91225.1. Sequence problems.
RefSeqiNP_001013065.1. NM_001013047.1.
UniGeneiRn.23921.

Genome annotation databases

GeneIDi288762.
KEGGirno:288762.
UCSCiRGD:1304582. rat. [Q6AXQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079400 mRNA. Translation: AAH79400.1. Different initiation.
BC091225 mRNA. Translation: AAH91225.1. Sequence problems.
RefSeqiNP_001013065.1. NM_001013047.1.
UniGeneiRn.23921.

3D structure databases

ProteinModelPortaliQ6AXQ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058761.

PTM databases

iPTMnetiQ6AXQ4.
PhosphoSitePlusiQ6AXQ4.

Proteomic databases

PaxDbiQ6AXQ4.
PRIDEiQ6AXQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi288762.
KEGGirno:288762.
UCSCiRGD:1304582. rat. [Q6AXQ4-1]

Organism-specific databases

CTDi4534.
RGDi1304582. Mtm1.

Phylogenomic databases

eggNOGiKOG4471. Eukaryota.
ENOG410XPTU. LUCA.
HOGENOMiHOG000210598.
HOVERGENiHBG000220.
InParanoidiQ6AXQ4.
KOiK01108.
OrthoDBiEOG091G04DS.
PhylomeDBiQ6AXQ4.

Miscellaneous databases

PROiQ6AXQ4.

Gene expression databases

BgeeiENSRNOG00000002516.
GenevisibleiQ6AXQ4. RN.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR004182. GRAM.
IPR010569. Myotubularin-like_Pase_dom.
IPR030564. Myotubularin_fam.
IPR011993. PH_dom-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
[Graphical view]
PANTHERiPTHR10807. PTHR10807. 1 hit.
PfamiPF02893. GRAM. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTM1_RAT
AccessioniPrimary (citable) accession number: Q6AXQ4
Secondary accession number(s): Q5BK31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.