Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SUMO-activating enzyme subunit 1

Gene

Sae1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity).By similarity

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-3065676. SUMO is conjugated to E1 (UBA2:SAE1).
R-RNO-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-activating enzyme subunit 1
Alternative name(s):
Ubiquitin-like 1-activating enzyme E1A
Cleaved into the following chain:
Gene namesi
Name:Sae1
Synonyms:Uble1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1306098. Sae1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349SUMO-activating enzyme subunit 1PRO_0000268868Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 349348SUMO-activating enzyme subunit 1, N-terminally processedPRO_0000423293Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylvaline; in SUMO-activating enzyme subunit 1, N-terminally processedBy similarity
Modified residuei201 – 2011N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ6AXQ0.
PRIDEiQ6AXQ0.

PTM databases

iPTMnetiQ6AXQ0.
PhosphoSiteiQ6AXQ0.

Expressioni

Gene expression databases

GenevisibleiQ6AXQ0. RN.

Interactioni

Subunit structurei

Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi258986. 1 interaction.
STRINGi10116.ENSRNOP00000020402.

Structurei

3D structure databases

ProteinModelPortaliQ6AXQ0.
SMRiQ6AXQ0. Positions 12-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Phylogenomic databases

eggNOGiKOG2014. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00550000075007.
HOGENOMiHOG000172217.
HOVERGENiHBG080782.
InParanoidiQ6AXQ0.
KOiK10684.
OMAiDQLCSKH.
OrthoDBiEOG7FR7GW.
PhylomeDBiQ6AXQ0.
TreeFamiTF315037.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SUPFAMiSSF69572. SSF69572. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AXQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEKEEVSGG GGGISEEEAA QYDRQIRLWG LEAQKRLRAS RVLIVGMKGL
60 70 80 90 100
GAEIAKNLIL AGVKGLTMLD HEQVSPEDLG AQFLIRTGSV GQNRAEASLE
110 120 130 140 150
RAQNLNPMVD VKVDTEDIEK KPESFFTEFD AVCLTCCSKD VIIKVDQICH
160 170 180 190 200
RNSIKFFTGD VFGYHGYTFA NLGEHEFVEE KTKVTKVSQG VEDGPDAKRA
210 220 230 240 250
KLDSSETTMV KKKVLFCPVK EALAVDWSGE KAQAALKRTA PDYFLLQVLL
260 270 280 290 300
KFRTDKGRDP TSDSYSEDAE LLLQIRNDVF DSLGVSPDLL PDDFVRYCFS
310 320 330 340
EMAPVCAVVG GILAQEIVKA LSQRDPPHNN FFFFDGMKGS GIVECLGPQ
Length:349
Mass (Da):38,513
Last modified:September 13, 2004 - v1
Checksum:iEBCF5C53436B2DDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079411 mRNA. Translation: AAH79411.1.
RefSeqiNP_001012063.1. NM_001012063.1.
UniGeneiRn.9014.

Genome annotation databases

EnsembliENSRNOT00000020402; ENSRNOP00000020402; ENSRNOG00000015128.
GeneIDi308384.
KEGGirno:308384.
UCSCiRGD:1306098. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079411 mRNA. Translation: AAH79411.1.
RefSeqiNP_001012063.1. NM_001012063.1.
UniGeneiRn.9014.

3D structure databases

ProteinModelPortaliQ6AXQ0.
SMRiQ6AXQ0. Positions 12-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi258986. 1 interaction.
STRINGi10116.ENSRNOP00000020402.

PTM databases

iPTMnetiQ6AXQ0.
PhosphoSiteiQ6AXQ0.

Proteomic databases

PaxDbiQ6AXQ0.
PRIDEiQ6AXQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020402; ENSRNOP00000020402; ENSRNOG00000015128.
GeneIDi308384.
KEGGirno:308384.
UCSCiRGD:1306098. rat.

Organism-specific databases

CTDi10055.
RGDi1306098. Sae1.

Phylogenomic databases

eggNOGiKOG2014. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00550000075007.
HOGENOMiHOG000172217.
HOVERGENiHBG080782.
InParanoidiQ6AXQ0.
KOiK10684.
OMAiDQLCSKH.
OrthoDBiEOG7FR7GW.
PhylomeDBiQ6AXQ0.
TreeFamiTF315037.

Enzyme and pathway databases

UniPathwayiUPA00886.
ReactomeiR-RNO-3065676. SUMO is conjugated to E1 (UBA2:SAE1).
R-RNO-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).

Miscellaneous databases

NextBioi658735.
PROiQ6AXQ0.

Gene expression databases

GenevisibleiQ6AXQ0. RN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SUPFAMiSSF69572. SSF69572. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiSAE1_RAT
AccessioniPrimary (citable) accession number: Q6AXQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 13, 2004
Last modified: May 11, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.