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Protein

Small kinetochore-associated protein

Gene

Knstrn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential component of the mitotic spindle required for faithful chromosome segregation and progression into anaphase. Promotes the metaphase-to-anaphase transition and is required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments. Required for kinetochore oscillations and dynamics of microtubule plus-ends during live cell mitosis, possibly by forming a link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Small kinetochore-associated protein
Short name:
SKAP
Alternative name(s):
Kinetochore-localized astrin-binding protein
Short name:
Kinastrin
Kinetochore-localized astrin/SPAG5-binding protein
TRAF4-associated factor 1
Gene namesi
Name:Knstrn
Synonyms:Skap, Traf4af1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1303240. Knstrn.

Subcellular locationi

  • Nucleus By similarity
  • Chromosomecentromerekinetochore By similarity
  • Cytoplasmcytoskeletonspindle pole By similarity

  • Note: Colocalizes with microtubules around centrosomes in prophase and with the mitotic spindle at prometaphase and metaphase. From late prometaphase to anaphase, is highly concentrated on kinetochores. Located at the kinetochore-microtubule interface. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745141 – 312Small kinetochore-associated proteinAdd BLAST312

Proteomic databases

PaxDbiQ6AXN6.
PRIDEiQ6AXN6.

PTM databases

iPTMnetiQ6AXN6.
PhosphoSitePlusiQ6AXN6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009334.
GenevisibleiQ6AXN6. RN.

Interactioni

Subunit structurei

Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGO2. Interacts with SPAG5. Directly binds to microtubules, although at relatively low affinity. Interacts with CENPE; this interaction greatly favors microtubule-binding. Interacts with DSN1/MIS13; leading to localization to kinetochores. Interacts with MAPRE1/EB1; leading to localization to the microtubule plus ends. Interacts with PRPF19.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012454.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 312Interaction with SPAG5By similarityAdd BLAST157

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili169 – 210Sequence analysisAdd BLAST42
Coiled coili246 – 288Sequence analysisAdd BLAST43

Domaini

The coiled coil regions mediate binding to kinetochores.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IKUT. Eukaryota.
ENOG4111DW5. LUCA.
GeneTreeiENSGT00390000010376.
HOGENOMiHOG000246953.
HOVERGENiHBG083924.
InParanoidiQ6AXN6.
OMAiQESTTDH.
OrthoDBiEOG091G0J0K.
PhylomeDBiQ6AXN6.
TreeFamiTF336302.

Family and domain databases

InterProiIPR033373. SKAP.
[Graphical view]
PANTHERiPTHR31940. PTHR31940. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AXN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATHKAEAQE TDFRTTGPPT DLEQCPFPPS SRKFPFESVA ADSTEGWAVA
60 70 80 90 100
AEHHLKRSGE DGGWEQPASG VEPSRPTTMA SSKTVCDAQP HSMPSCGLSA
110 120 130 140 150
DTQTRATSKL PVKSKDAEML RHLHTGGLEP DVTKVTKPRR ENGQGKAAET
160 170 180 190 200
ASRRNIRSSY KPLSKQKPEE DLKDKNELLE AVNKQLHQKL TETQGELKDL
210 220 230 240 250
TQKVELLEKF QDNCLAILES KGLNSGQETQ ESKQEPSTDP TDSMLLLETL
260 270 280 290 300
KDELKLFNET AKKQMEELQA LKVKLKLKEK ERIQFLEQQT LGKDEASDFT
310
IILEEMEQLL EM
Length:312
Mass (Da):34,885
Last modified:September 13, 2004 - v1
Checksum:i313382501B56AD2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079438 mRNA. Translation: AAH79438.1.
RefSeqiNP_001004264.1. NM_001004264.1.
UniGeneiRn.20235.

Genome annotation databases

EnsembliENSRNOT00000012454; ENSRNOP00000012454; ENSRNOG00000009334.
GeneIDi311325.
KEGGirno:311325.
UCSCiRGD:1303240. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079438 mRNA. Translation: AAH79438.1.
RefSeqiNP_001004264.1. NM_001004264.1.
UniGeneiRn.20235.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012454.

PTM databases

iPTMnetiQ6AXN6.
PhosphoSitePlusiQ6AXN6.

Proteomic databases

PaxDbiQ6AXN6.
PRIDEiQ6AXN6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012454; ENSRNOP00000012454; ENSRNOG00000009334.
GeneIDi311325.
KEGGirno:311325.
UCSCiRGD:1303240. rat.

Organism-specific databases

CTDi90417.
RGDi1303240. Knstrn.

Phylogenomic databases

eggNOGiENOG410IKUT. Eukaryota.
ENOG4111DW5. LUCA.
GeneTreeiENSGT00390000010376.
HOGENOMiHOG000246953.
HOVERGENiHBG083924.
InParanoidiQ6AXN6.
OMAiQESTTDH.
OrthoDBiEOG091G0J0K.
PhylomeDBiQ6AXN6.
TreeFamiTF336302.

Miscellaneous databases

PROiQ6AXN6.

Gene expression databases

BgeeiENSRNOG00000009334.
GenevisibleiQ6AXN6. RN.

Family and domain databases

InterProiIPR033373. SKAP.
[Graphical view]
PANTHERiPTHR31940. PTHR31940. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSKAP_RAT
AccessioniPrimary (citable) accession number: Q6AXN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.