Reviewed, UniProtKB/Swiss-Prot Q6AXM8 (PON2_RAT)

Names and origin

Protein names

Recommended name:
    Serum paraoxonase/arylesterase 2
      Short name=PON 2
    EC=3.1.1.2
    EC=3.1.8.1
Alternative name(s):
    Serum aryldialkylphosphatase 2
    Aromatic esterase 2
      Short name=A-esterase 2

Gene names

Name:

Pon2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters By similarity.
Catalytic activity	A phenyl acetate + H2O = a phenol + acetate. An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Post-translational modification	Glycosylated By similarity. The signal sequence is not cleaved By similarity.
Sequence similarities	Belongs to the paraoxonase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	response to oxidative stress Inferred from expression pattern. Source: RGD
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from direct assay. Source: RGD microsome Inferred from direct assay. Source: RGD mitochondrion Inferred from direct assay. Source: RGD nucleus Inferred from direct assay. Source: RGD
Molecular function	aryldialkylphosphatase activity Inferred from electronic annotation. Source: EC arylesterase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Serum paraoxonase/arylesterase 2		PRO_0000223289
	Signal peptide	1 – ?		Not cleaved Potential
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	53	1	Calcium 1; catalytic By similarity
	Metal binding	54	1	Calcium 2 By similarity
	Metal binding	116	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	167	1	Calcium 1; catalytic By similarity
	Metal binding	168	1	Calcium 2 By similarity
	Metal binding	223	1	Calcium 1; catalytic By similarity
	Metal binding	268	1	Calcium 1; catalytic By similarity
	Metal binding	269	1	Calcium 1; catalytic By similarity
Amino acid modifications
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 352		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AXM8-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 69D77678C781859D	FASTA	354	39,617
	10 20 30 40 50 60 MGRLVALSLL GIGLALLGER FLALRSRLKA SREVESVDLP NCRLIKGIET GAEDIDILPN 70 80 90 100 110 120 GLAFFSVGLK FPGLHSFAPD KPGGILMMDL KEEKPRALEL RVSWGFDLAS FNPHGISTFI 130 140 150 160 170 180 DDDDTVYLFV VNHPEFKNTV EIFKFQEEEN SLLHLKTIKH ELLPSVNDVI AVGPSHFYAT 190 200 210 220 230 240 NDHYFSDPFL KYLETYLNLR WANVVYYSPE EVKLVAEGFD SANGINISPD KKYVYVADIL 250 260 270 280 290 300 AHEIHVLEKQ PNMNLTQLKV LQLGTLVDNL SIDPSSGDIW VGCHPNGQKL FVYDPNHPPS 310 320 330 340 350 SEVLRIQNIL SEKPSVTTVY INNGSVLQGS SVATIYDRKL LVGTLYQRAL YCEL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079462 mRNA. Translation: AAH79462.1.
IPI	IPI00464486.
RefSeq	NP_001013100.1.
UniGene	Rn.1100
3D structure databases
SMR	Q6AXM8. Positions 23-354.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AXM8.
Genome annotation databases
Ensembl	ENSRNOT00000036460; ENSRNOP00000033943; ENSRNOG00000009112; Rattus norvegicus. [Genome view]
GeneID	296851.
KEGG	rno:296851.
NMPDR	fig|10116.3.peg.20803.
UCSC	NM_001013082. rat.
Organism-specific databases
CTD	296851.
RGD	1309954. Pon2.
Phylogenomic databases
eggNOG	roNOG06389.
HOVERGEN	Q6AXM8.
InParanoid	Q6AXM8.
OMA	TIEIFER.
OrthoDB	EOG912PQX.
PhylomeDB	Q6AXM8.
Enzyme and pathway databases
BRENDA	3.1.1.2. 248. 3.1.8.1. 248.
Gene expression databases
ArrayExpress	Q6AXM8.
Genevestigator	Q6AXM8.
GermOnline	ENSRNOG00000009112. Rattus norvegicus.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR002640. Arylesterase. IPR008364. Paraoxonase2. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
Pfam	PF01731. Arylesterase. 1 hit. [Graphical view]
PRINTS	PR01785. PARAOXONASE. PR01787. PARAOXONASE2.
ProtoNet	Search...
Other Resources
NextBio	641839.

Entry information

Entry name

PON2_RAT

Accession

Primary (citable) accession number: Q6AXM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	September 13, 2004
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)