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Q6AXC6 (DDX11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent RNA helicase DDX11

EC=3.6.4.13
Alternative name(s):
DEAD/H box protein 11
Gene names
Name:Ddx11
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length880 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA By similarity. Ref.2

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Binds 1 4Fe-4S cluster By similarity.

Subunit structure

Interacts with the CTF18-RFC complex, PCNA and FEN1. Forms a complex with RAD21, SMC1 and SMC3 By similarity.

Subcellular location

Nucleus By similarity. Nucleusnucleolus By similarity. Note: During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase) By similarity.

Disruption phenotype

Embryonic death at E10.5. Embryos are smaller in size, malformed and exhibit sparse cellularity in comparison to normal or heterozygous litter mates. Loss of Ddx11 results in the inability to form a proper placenta, indicating that it is essential for placental formation. Ref.2

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.

Contains 1 helicase ATP-binding domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 880880Probable ATP-dependent RNA helicase DDX11
PRO_0000055137

Regions

Domain9 – 416408Helicase ATP-binding
Nucleotide binding44 – 518ATP By similarity
Motif364 – 3674DEAH
Compositional bias121 – 19676Glu-rich

Sites

Metal binding2391Iron-sulfur (4Fe-4S) By similarity
Metal binding2571Iron-sulfur (4Fe-4S) By similarity
Metal binding2861Iron-sulfur (4Fe-4S) By similarity
Metal binding3211Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6AXC6 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 0A283D952B974F82

FASTA88098,728
        10         20         30         40         50         60 
MADENQEIGG IHFPFPFPPY PIQKDFMAEL YKVLEGGKIG IFESPTGTGK SLSLICGALS 

        70         80         90        100        110        120 
WLRDFEKKKL QAEALLLAPG SGPPSSEKNS LLTSSSCQEP TDTPRPAGEP DWVTEFVQKK 

       130        140        150        160        170        180 
EERDLVERLR EEQKHEEEET EALLRLSREM LDAGTGPEQL EQLECGEEHL VLAEYESDEE 

       190        200        210        220        230        240 
RRGSRVDEAE DDLEEEHITK IYYCSRTHSQ LAQFVREVLK SPFGKETRLV SLGSRQTLCV 

       250        260        270        280        290        300 
NEDVKNLGSV QLMNDRCVDM QRSKREKNGT GEDKPKRKRQ KIQTSCPFYN HEQMELLRDE 

       310        320        330        340        350        360 
ILLEVKDMEQ LVALGKEARA CPYYGSRFAI PAAQLVVLPY PMLLHAATRQ AAGIRLQGQV 

       370        380        390        400        410        420 
VIIDEAHNLI DTITNIHSTE VNGSQLCQAH SQLLQYMERY RKRLKAKNLM YIKQILYLLE 

       430        440        450        460        470        480 
KFVAVLGGNV KQNPTTQSLS QTGSELKSIN DFLFQSQVDN INLFKVQRYL EKSMLSRKLF 

       490        500        510        520        530        540 
GFTECFGVVL PSLSDSQENR GLAGFQQFLK SLQSGPTEDS PEEGQAVALR PASPLMHIEA 

       550        560        570        580        590        600 
FLAALTTANQ DGRVIVNRQG SVGQSSLKFL LLNPAVHFAQ VVKECRAVVI AGGTMQPMSD 

       610        620        630        640        650        660 
FREQLLACSG VEAGRVVEFS CGHVIPPDNI LPLIICSGPS NQQLEFTYQR RELPQMVEET 

       670        680        690        700        710        720 
GRILCNLCNV VPGGVVCFLP SYEYLRQVHA HWDKTGLLTR LSVRKKIFQE PKRASQVEQV 

       730        740        750        760        770        780 
LMAYSKCIMS CSHSEGHLTG ALLLSVVGGK MSEGINFSDD LGRCVVMVGM PYPNIKSPEL 

       790        800        810        820        830        840 
QEKMAYLNQT LPRTQGQPLP GTVLIENLCM KAINQSIGRA IRHQRDFASI VLLDHRYARP 

       850        860        870        880 
SILAKLPAWI RDRVEVKATF GPAFAAVRKF HREKSHPSLV 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[2]"Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation."
Inoue A., Li T., Roby S.K., Valentine M.B., Inoue M., Boyd K., Kidd V.J., Lahti J.M.
Cell Cycle 6:1646-1654(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC079656 mRNA. Translation: AAH79656.1.
RefSeqNP_001003919.1. NM_001003919.1.
UniGeneMm.259605.

3D structure databases

ProteinModelPortalQ6AXC6.
SMRQ6AXC6. Positions 17-62, 196-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000039225.

PTM databases

PhosphoSiteQ6AXC6.

Proteomic databases

PaxDbQ6AXC6.
PRIDEQ6AXC6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000163605; ENSMUSP00000130440; ENSMUSG00000035842.
GeneID320209.
KEGGmmu:320209.
UCSCuc008dha.1. mouse.

Organism-specific databases

CTD1663.
MGIMGI:2443590. Ddx11.

Phylogenomic databases

eggNOGCOG1199.
GeneTreeENSGT00530000063199.
HOGENOMHOG000241266.
HOVERGENHBG058884.
InParanoidQ6AXC6.
KOK11273.
OMAELYENMC.
OrthoDBEOG7ZD1TW.
PhylomeDBQ6AXC6.
TreeFamTF300435.

Gene expression databases

BgeeQ6AXC6.
GenevestigatorQ6AXC6.

Family and domain databases

Gene3D3.40.50.300. 3 hits.
InterProIPR006555. ATP-dep_Helicase_C.
IPR028331. DDX11-like.
IPR010614. DEAD_2.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11472:SF20. PTHR11472:SF20. 1 hit.
PfamPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00604. rad3. 1 hit.
PROSITEPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio396263.
PROQ6AXC6.
SOURCESearch...

Entry information

Entry nameDDX11_MOUSE
AccessionPrimary (citable) accession number: Q6AXC6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: September 13, 2004
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot