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Protein

Probable ATP-dependent RNA helicase DDX11

Gene

Ddx11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi239 – 2391Iron-sulfur (4Fe-4S)By similarity
Metal bindingi257 – 2571Iron-sulfur (4Fe-4S)By similarity
Metal bindingi286 – 2861Iron-sulfur (4Fe-4S)By similarity
Metal bindingi321 – 3211Iron-sulfur (4Fe-4S)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 518ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX11 (EC:3.6.4.13)
Alternative name(s):
DEAD/H box protein 11
Gene namesi
Name:Ddx11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2443590. Ddx11.

Subcellular locationi

  • Nucleus By similarity
  • Nucleusnucleolus By similarity

  • Note: During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase) (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic death at E10.5. Embryos are smaller in size, malformed and exhibit sparse cellularity in comparison to normal or heterozygous litter mates. Loss of Ddx11 results in the inability to form a proper placenta, indicating that it is essential for placental formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 880880Probable ATP-dependent RNA helicase DDX11PRO_0000055137Add
BLAST

Proteomic databases

MaxQBiQ6AXC6.
PaxDbiQ6AXC6.
PRIDEiQ6AXC6.

PTM databases

PhosphoSiteiQ6AXC6.

Expressioni

Gene expression databases

BgeeiQ6AXC6.
GenevisibleiQ6AXC6. MM.

Interactioni

Subunit structurei

Interacts with the CTF18-RFC complex, PCNA and FEN1. Forms a complex with RAD21, SMC1 and SMC3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130440.

Structurei

3D structure databases

ProteinModelPortaliQ6AXC6.
SMRiQ6AXC6. Positions 18-59, 196-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 416408Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi364 – 3674DEAH

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi121 – 19676Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1199.
GeneTreeiENSGT00530000063199.
HOGENOMiHOG000241266.
HOVERGENiHBG058884.
InParanoidiQ6AXC6.
KOiK11273.
OMAiENLCMRA.
OrthoDBiEOG7ZD1TW.
PhylomeDBiQ6AXC6.
TreeFamiTF300435.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR028331. DDX11/DDX12.
IPR010614. DEAD_2.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11472:SF35. PTHR11472:SF35. 1 hit.
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AXC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADENQEIGG IHFPFPFPPY PIQKDFMAEL YKVLEGGKIG IFESPTGTGK
60 70 80 90 100
SLSLICGALS WLRDFEKKKL QAEALLLAPG SGPPSSEKNS LLTSSSCQEP
110 120 130 140 150
TDTPRPAGEP DWVTEFVQKK EERDLVERLR EEQKHEEEET EALLRLSREM
160 170 180 190 200
LDAGTGPEQL EQLECGEEHL VLAEYESDEE RRGSRVDEAE DDLEEEHITK
210 220 230 240 250
IYYCSRTHSQ LAQFVREVLK SPFGKETRLV SLGSRQTLCV NEDVKNLGSV
260 270 280 290 300
QLMNDRCVDM QRSKREKNGT GEDKPKRKRQ KIQTSCPFYN HEQMELLRDE
310 320 330 340 350
ILLEVKDMEQ LVALGKEARA CPYYGSRFAI PAAQLVVLPY PMLLHAATRQ
360 370 380 390 400
AAGIRLQGQV VIIDEAHNLI DTITNIHSTE VNGSQLCQAH SQLLQYMERY
410 420 430 440 450
RKRLKAKNLM YIKQILYLLE KFVAVLGGNV KQNPTTQSLS QTGSELKSIN
460 470 480 490 500
DFLFQSQVDN INLFKVQRYL EKSMLSRKLF GFTECFGVVL PSLSDSQENR
510 520 530 540 550
GLAGFQQFLK SLQSGPTEDS PEEGQAVALR PASPLMHIEA FLAALTTANQ
560 570 580 590 600
DGRVIVNRQG SVGQSSLKFL LLNPAVHFAQ VVKECRAVVI AGGTMQPMSD
610 620 630 640 650
FREQLLACSG VEAGRVVEFS CGHVIPPDNI LPLIICSGPS NQQLEFTYQR
660 670 680 690 700
RELPQMVEET GRILCNLCNV VPGGVVCFLP SYEYLRQVHA HWDKTGLLTR
710 720 730 740 750
LSVRKKIFQE PKRASQVEQV LMAYSKCIMS CSHSEGHLTG ALLLSVVGGK
760 770 780 790 800
MSEGINFSDD LGRCVVMVGM PYPNIKSPEL QEKMAYLNQT LPRTQGQPLP
810 820 830 840 850
GTVLIENLCM KAINQSIGRA IRHQRDFASI VLLDHRYARP SILAKLPAWI
860 870 880
RDRVEVKATF GPAFAAVRKF HREKSHPSLV
Length:880
Mass (Da):98,728
Last modified:September 13, 2004 - v1
Checksum:i0A283D952B974F82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079656 mRNA. Translation: AAH79656.1.
CCDSiCCDS28945.1.
RefSeqiNP_001003919.1. NM_001003919.1.
UniGeneiMm.259605.

Genome annotation databases

EnsembliENSMUST00000163605; ENSMUSP00000130440; ENSMUSG00000035842.
GeneIDi320209.
KEGGimmu:320209.
UCSCiuc008dha.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079656 mRNA. Translation: AAH79656.1.
CCDSiCCDS28945.1.
RefSeqiNP_001003919.1. NM_001003919.1.
UniGeneiMm.259605.

3D structure databases

ProteinModelPortaliQ6AXC6.
SMRiQ6AXC6. Positions 18-59, 196-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130440.

PTM databases

PhosphoSiteiQ6AXC6.

Proteomic databases

MaxQBiQ6AXC6.
PaxDbiQ6AXC6.
PRIDEiQ6AXC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000163605; ENSMUSP00000130440; ENSMUSG00000035842.
GeneIDi320209.
KEGGimmu:320209.
UCSCiuc008dha.1. mouse.

Organism-specific databases

CTDi1663.
MGIiMGI:2443590. Ddx11.

Phylogenomic databases

eggNOGiCOG1199.
GeneTreeiENSGT00530000063199.
HOGENOMiHOG000241266.
HOVERGENiHBG058884.
InParanoidiQ6AXC6.
KOiK11273.
OMAiENLCMRA.
OrthoDBiEOG7ZD1TW.
PhylomeDBiQ6AXC6.
TreeFamiTF300435.

Miscellaneous databases

NextBioi396263.
PROiQ6AXC6.
SOURCEiSearch...

Gene expression databases

BgeeiQ6AXC6.
GenevisibleiQ6AXC6. MM.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR028331. DDX11/DDX12.
IPR010614. DEAD_2.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11472:SF35. PTHR11472:SF35. 1 hit.
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation."
    Inoue A., Li T., Roby S.K., Valentine M.B., Inoue M., Boyd K., Kidd V.J., Lahti J.M.
    Cell Cycle 6:1646-1654(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiDDX11_MOUSE
AccessioniPrimary (citable) accession number: Q6AXC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: September 13, 2004
Last modified: June 24, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.