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Q6AXC6

- DDX11_MOUSE

UniProt

Q6AXC6 - DDX11_MOUSE

Protein

Probable ATP-dependent RNA helicase DDX11

Gene

Ddx11

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Binds 1 4Fe-4S cluster.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi239 – 2391Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi257 – 2571Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi286 – 2861Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi321 – 3211Iron-sulfur (4Fe-4S)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi44 – 518ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. ATP binding Source: UniProtKB-KW
    3. ATP-dependent DNA helicase activity Source: InterPro
    4. DNA binding Source: UniProtKB-KW
    5. DNA-dependent ATPase activity Source: UniProtKB
    6. helicase activity Source: UniProtKB
    7. metal ion binding Source: UniProtKB-KW
    8. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. sister chromatid cohesion Source: UniProtKB

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_106572. XBP1(S) activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent RNA helicase DDX11 (EC:3.6.4.13)
    Alternative name(s):
    DEAD/H box protein 11
    Gene namesi
    Name:Ddx11
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:2443590. Ddx11.

    Subcellular locationi

    Nucleus By similarity. Nucleusnucleolus By similarity
    Note: During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase) By similarity.By similarity

    GO - Cellular componenti

    1. midbody Source: UniProtKB
    2. nuclear chromatin Source: UniProtKB
    3. nucleolus Source: UniProtKB-SubCell
    4. spindle pole Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Embryonic death at E10.5. Embryos are smaller in size, malformed and exhibit sparse cellularity in comparison to normal or heterozygous litter mates. Loss of Ddx11 results in the inability to form a proper placenta, indicating that it is essential for placental formation.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 880880Probable ATP-dependent RNA helicase DDX11PRO_0000055137Add
    BLAST

    Proteomic databases

    MaxQBiQ6AXC6.
    PaxDbiQ6AXC6.
    PRIDEiQ6AXC6.

    PTM databases

    PhosphoSiteiQ6AXC6.

    Expressioni

    Gene expression databases

    BgeeiQ6AXC6.
    GenevestigatoriQ6AXC6.

    Interactioni

    Subunit structurei

    Interacts with the CTF18-RFC complex, PCNA and FEN1. Forms a complex with RAD21, SMC1 and SMC3 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000039225.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6AXC6.
    SMRiQ6AXC6. Positions 15-59, 196-396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 416408Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi364 – 3674DEAH

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi121 – 19676Glu-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1199.
    GeneTreeiENSGT00530000063199.
    HOGENOMiHOG000241266.
    HOVERGENiHBG058884.
    InParanoidiQ6AXC6.
    KOiK11273.
    OMAiELYENMC.
    OrthoDBiEOG7ZD1TW.
    PhylomeDBiQ6AXC6.
    TreeFamiTF300435.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR006555. ATP-dep_Helicase_C.
    IPR028331. DDX11/DDX12.
    IPR010614. DEAD_2.
    IPR013020. DNA_helicase_DNA-repair_Rad3.
    IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
    IPR006554. Helicase-like_DEXD_c2.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11472:SF35. PTHR11472:SF35. 1 hit.
    PfamiPF06733. DEAD_2. 1 hit.
    PF13307. Helicase_C_2. 1 hit.
    [Graphical view]
    SMARTiSM00488. DEXDc2. 1 hit.
    SM00491. HELICc2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    TIGRFAMsiTIGR00604. rad3. 1 hit.
    PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6AXC6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADENQEIGG IHFPFPFPPY PIQKDFMAEL YKVLEGGKIG IFESPTGTGK    50
    SLSLICGALS WLRDFEKKKL QAEALLLAPG SGPPSSEKNS LLTSSSCQEP 100
    TDTPRPAGEP DWVTEFVQKK EERDLVERLR EEQKHEEEET EALLRLSREM 150
    LDAGTGPEQL EQLECGEEHL VLAEYESDEE RRGSRVDEAE DDLEEEHITK 200
    IYYCSRTHSQ LAQFVREVLK SPFGKETRLV SLGSRQTLCV NEDVKNLGSV 250
    QLMNDRCVDM QRSKREKNGT GEDKPKRKRQ KIQTSCPFYN HEQMELLRDE 300
    ILLEVKDMEQ LVALGKEARA CPYYGSRFAI PAAQLVVLPY PMLLHAATRQ 350
    AAGIRLQGQV VIIDEAHNLI DTITNIHSTE VNGSQLCQAH SQLLQYMERY 400
    RKRLKAKNLM YIKQILYLLE KFVAVLGGNV KQNPTTQSLS QTGSELKSIN 450
    DFLFQSQVDN INLFKVQRYL EKSMLSRKLF GFTECFGVVL PSLSDSQENR 500
    GLAGFQQFLK SLQSGPTEDS PEEGQAVALR PASPLMHIEA FLAALTTANQ 550
    DGRVIVNRQG SVGQSSLKFL LLNPAVHFAQ VVKECRAVVI AGGTMQPMSD 600
    FREQLLACSG VEAGRVVEFS CGHVIPPDNI LPLIICSGPS NQQLEFTYQR 650
    RELPQMVEET GRILCNLCNV VPGGVVCFLP SYEYLRQVHA HWDKTGLLTR 700
    LSVRKKIFQE PKRASQVEQV LMAYSKCIMS CSHSEGHLTG ALLLSVVGGK 750
    MSEGINFSDD LGRCVVMVGM PYPNIKSPEL QEKMAYLNQT LPRTQGQPLP 800
    GTVLIENLCM KAINQSIGRA IRHQRDFASI VLLDHRYARP SILAKLPAWI 850
    RDRVEVKATF GPAFAAVRKF HREKSHPSLV 880
    Length:880
    Mass (Da):98,728
    Last modified:September 13, 2004 - v1
    Checksum:i0A283D952B974F82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC079656 mRNA. Translation: AAH79656.1.
    CCDSiCCDS28945.1.
    RefSeqiNP_001003919.1. NM_001003919.1.
    UniGeneiMm.259605.

    Genome annotation databases

    EnsembliENSMUST00000163605; ENSMUSP00000130440; ENSMUSG00000035842.
    GeneIDi320209.
    KEGGimmu:320209.
    UCSCiuc008dha.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC079656 mRNA. Translation: AAH79656.1 .
    CCDSi CCDS28945.1.
    RefSeqi NP_001003919.1. NM_001003919.1.
    UniGenei Mm.259605.

    3D structure databases

    ProteinModelPortali Q6AXC6.
    SMRi Q6AXC6. Positions 15-59, 196-396.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000039225.

    PTM databases

    PhosphoSitei Q6AXC6.

    Proteomic databases

    MaxQBi Q6AXC6.
    PaxDbi Q6AXC6.
    PRIDEi Q6AXC6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000163605 ; ENSMUSP00000130440 ; ENSMUSG00000035842 .
    GeneIDi 320209.
    KEGGi mmu:320209.
    UCSCi uc008dha.1. mouse.

    Organism-specific databases

    CTDi 1663.
    MGIi MGI:2443590. Ddx11.

    Phylogenomic databases

    eggNOGi COG1199.
    GeneTreei ENSGT00530000063199.
    HOGENOMi HOG000241266.
    HOVERGENi HBG058884.
    InParanoidi Q6AXC6.
    KOi K11273.
    OMAi ELYENMC.
    OrthoDBi EOG7ZD1TW.
    PhylomeDBi Q6AXC6.
    TreeFami TF300435.

    Enzyme and pathway databases

    Reactomei REACT_106572. XBP1(S) activates chaperone genes.

    Miscellaneous databases

    NextBioi 396263.
    PROi Q6AXC6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6AXC6.
    Genevestigatori Q6AXC6.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR006555. ATP-dep_Helicase_C.
    IPR028331. DDX11/DDX12.
    IPR010614. DEAD_2.
    IPR013020. DNA_helicase_DNA-repair_Rad3.
    IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
    IPR006554. Helicase-like_DEXD_c2.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11472:SF35. PTHR11472:SF35. 1 hit.
    Pfami PF06733. DEAD_2. 1 hit.
    PF13307. Helicase_C_2. 1 hit.
    [Graphical view ]
    SMARTi SM00488. DEXDc2. 1 hit.
    SM00491. HELICc2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    TIGRFAMsi TIGR00604. rad3. 1 hit.
    PROSITEi PS51193. HELICASE_ATP_BIND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation."
      Inoue A., Li T., Roby S.K., Valentine M.B., Inoue M., Boyd K., Kidd V.J., Lahti J.M.
      Cell Cycle 6:1646-1654(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.

    Entry informationi

    Entry nameiDDX11_MOUSE
    AccessioniPrimary (citable) accession number: Q6AXC6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3