Q6AWJ9 (PTK7_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase-like otk

Alternative name(s):
Gp160-Dtrk
Short name=Dtrk
Off-track
Tyrosine-protein kinase-like 7 homolog

Gene names

Name:	otk
ORF Names:	CG8967

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a calcium-dependent, homophilic cell adhesion molecule that regulates neural recognition during the development of the nervous system. Component of the repulsive Plexin signaling response to regulate motor axon guidance at the embryonic stage. Also component of a receptor complex that is required in the adult visual system to innervate the lamina layer; specific targeting of R1-R6 axons. Ref.1 Ref.5 Ref.6
Subunit structure	Interacts with plexA; component of a receptor complex that mediates the repulsive signaling in response to Semaphorin ligands. Ref.5
Subcellular location	Cell membrane; Single-pass type I membrane protein Ref.1.
Tissue specificity	Dynamically expressed during embryogenesis in several areas of the developing nervous system, including neurons and fasciculating axons. Expression in stage 7 embryos is seen in the anterior midgut primordia, cephalic furrow and along the germinal band. At stage 11, expression is in 15 stripes over the trunk region, and in the anterior and posterior midgut primordia. Stage 12 shows expression in the developing nervous system, procephalic lobe and maxillar bud. Stage 13 shows expression in the ventral cord, maxillar segment and in three regions of the gut. At stage 16 expression is preferentially detected throughout the nervous system, including the neuromers in the ventral cord and the supraoesophageal ganglion (at protein level). In larva, expression is seen in developing R cells and is localized predominantly to R1-R6 growth cones. Ref.1 Ref.6
Developmental stage	Expressed both maternally and zygotically, during early to mid embryogenesis and early pupation. Ref.1
Disruption phenotype	Axon guidance defects. R-cell differentiation and cell fate determination are normal, but many R1-R6 axons connect abnormally to medulla instead of innervating lamina. Ref.5 Ref.6
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Caution	This protein has been proposed to undergo autophosphorylation on tyrosine residues which is induced in response to cell adhesion (Ref.1). However as mammalian orthologs of this protein seem to lack kinase activity this protein may associate with, and be phosphorylated by, an unknown active tyrosine kinase.

Ontologies

Keywords
Biological process	Cell adhesion Neurogenesis
Cellular component	Cell membrane Membrane
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB retinal ganglion cell axon guidance Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro cell adhesion molecule binding Inferred from sequence or structural similarity. Source: UniProtKB protein tyrosine kinase activity Non-traceable author statement. Source: FlyBase receptor activity Inferred from sequence or structural similarity. Source: UniProtKB semaphorin receptor binding Traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 1033	1011	Tyrosine-protein kinase-like otk		PRO_5000145927
Regions
	Topological domain	23 – 581	559	Extracellular Potential
	Transmembrane	582 – 602	21	Helical; Potential
	Topological domain	603 – 1033	431	Cytoplasmic Potential
	Domain	25 – 114	90	Ig-like C2-type 1
	Domain	113 – 199	87	Ig-like C2-type 2
	Domain	251 – 365	115	Ig-like C2-type 3
	Domain	368 – 463	96	Ig-like C2-type 4
	Domain	468 – 558	91	Ig-like C2-type 5
	Domain	692 – 1028	337	Protein kinase; inactive
Amino acid modifications
	Modified residue	678	1	Phosphoserine Ref.7
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	417	1	N-linked (GlcNAc...) Potential
	Glycosylation	429	1	N-linked (GlcNAc...) Potential
	Glycosylation	444	1	N-linked (GlcNAc...) Potential
	Glycosylation	457	1	N-linked (GlcNAc...) Potential
	Glycosylation	512	1	N-linked (GlcNAc...) Potential
	Glycosylation	524	1	N-linked (GlcNAc...) Ref.8
	Disulfide bond	46 ↔ 95		By similarity
	Disulfide bond	137 ↔ 188		By similarity
	Disulfide bond	276 ↔ 354		By similarity
	Disulfide bond	399 ↔ 447		By similarity
	Disulfide bond	490 ↔ 542		By similarity
Experimental info
	Sequence conflict	221	1	E → Q in CAA45053. Ref.1
	Sequence conflict	239	1	G → S in ACQ91629. Ref.4
	Sequence conflict	271	1	P → H in CAA45053. Ref.1
	Sequence conflict	311	1	I → F in CAA45053. Ref.1
	Sequence conflict	402	1	Q → L in ACQ91629. Ref.4
	Sequence conflict	629	1	V → I in CAA45053. Ref.1
	Sequence conflict	772	1	D → E in CAA45053. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6AWJ9 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 559659540BDE66F5	FASTA	1,033	114,267
	10 20 30 40 50 60 MTARMISICG LVMALMMASV LASSSRFQRV PQSQSVVENE SVKFECESTD SYSELHYDWL 70 80 90 100 110 120 HNGHRIAYDK RVHQIGSNLH IEAVRRTEDV GNYVCIATNL ASGAREASPP AKLSVIYLES 130 140 150 160 170 180 ASVQLLGSNR NELLLKCHVE GASGDLEPLE IEWYRNSEKL STWKNVQLDQ HRLIIRQPGS 190 200 210 220 230 240 EDDGLYRCTA SNAAGRVMSK QGYVYQSSVK CLPRLPRRKN EKMMESWDKQ TFLCRGKRGG 250 260 270 280 290 300 AAGLEALPAA PEDLRIVQGP IGQSIIKEGE PTALTCLYEL PDELKNQRIQ LRWRKDGKLL 310 320 330 340 350 360 RQVELGGSAP IPGHSFDSGK DALLREDARL VLHKQNGTLS FASIIASDAG QYQCQLQLEA 370 380 390 400 410 420 HAPINSSPGI LEVIEQLKFV PQPTSKNLEL DAVVAKVHCK AQGTPTPQVQ WVRDGENTTL 430 440 450 460 470 480 PDHVEVDANG TLIFRNVNSE HRGNYTCLAT NSQGQINATV AINVVVTPKF SVPPVGPIET 490 500 510 520 530 540 SEQGTVVMHC QAIGDPKPTI QWDKDLKYLS ENNTDRERFR FLENGTLEIR NVQVEDEGSY 550 560 570 580 590 600 GCTIGNSAGL KREDVQLVVK TTGDGFAPEE SGGDGFLVTR AVLITMTVAL AYIVLVVGLM 610 620 630 640 650 660 LWCRYRRQAR KARLNDLSTK EAGGDQPDVA GNGKGSEQEP CLSKQHNGHS KSRSKSSGDA 670 680 690 700 710 720 QKSDDTACSQ QSRASKKSAH IYEQLALPRS GLSELIQIGR GEFGDVFVGK LKATLVTSPS 730 740 750 760 770 780 DKDADTEKQH SNSENGSGGS GSGSTTLSTL NEKRRSKTSM DDIEEIKEEE QDQHNQSGLE 790 800 810 820 830 840 QLVLVKALNK VKDEQACQEF RRQLDLLRAI SHKGVVRLFG LCREKDPHYM VLEYTDWGDL 850 860 870 880 890 900 KQFLLATAGK VNTATAGSSS PPPLTTSQVL AVAYQIARGM DAIYRARFTH RDLATRNCVI 910 920 930 940 950 960 SSEFIVKVSY PALCKDKYSR EYHKHRNTLL PIRWLAPECI QEDEYTTKSD IFAYGVVVWE 970 980 990 1000 1010 1020 LFNQATKLPH EELTNEQVVQ RSQAGSLEWS VAEATPDSLR EILLSCWVSN PKERPSFSQL 1030 GAALSKAMQS AEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dtrk, a Drosophila gene related to the trk family of neurotrophin receptors, encodes a novel class of neural cell adhesion molecule." Pulido D., Campuzano S., Koda T., Modolell J., Barbacid M. EMBO J. 11:391-404(1992) [PubMed: 1371458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Canton-S. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Larva and Pupae.
[5]	"The transmembrane protein Off-track associates with Plexins and functions downstream of Semaphorin signaling during axon guidance." Winberg M.L., Tamagnone L., Bai J., Comoglio P.M., Montell D., Goodman C.S. Neuron 32:53-62(2001) [PubMed: 11604138] [Abstract] Cited for: FUNCTION, INTERACTION WITH PLEXA, DISRUPTION PHENOTYPE.
[6]	"The receptor tyrosine kinase Off-track is required for layer-specific neuronal connectivity in Drosophila." Cafferty P., Yu L., Rao Y. Development 131:5287-5295(2004) [PubMed: 15456725] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-678, MASS SPECTROMETRY. Tissue: Embryo.
[8]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-524, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63453 mRNA. Translation: CAA45053.1. AE013599 Genomic DNA. Translation: AAF58596.1. BT015249 mRNA. Translation: AAT94478.1. BT083425 mRNA. Translation: ACQ91629.1.
PIR	S19247.
RefSeq	NP_523705.2. NM_078981.2.
UniGene	Dm.2582.
3D structure databases
ProteinModelPortal	Q6AWJ9.
SMR	Q6AWJ9. Positions 23-205, 334-563, 683-1030.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AWJ9.
Proteomic databases
PRIDE	Q6AWJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0088028; FBpp0087135; FBgn0004839.
GeneID	36283.
KEGG	dme:Dmel_CG8967.
NMPDR	fig|7227.3.peg.4735.
UCSC	CG8967-RA. d. melanogaster.
Organism-specific databases
CTD	36283.
FlyBase	FBgn0004839. otk.
Phylogenomic databases
GeneTree	EMGT00050000001149.
InParanoid	Q6AWJ9.
OMA	SENNTDR.
OrthoDB	EOG4866TH.
PhylomeDB	Q6AWJ9.
Gene expression databases
ArrayExpress	Q6AWJ9.
Bgee	Q6AWJ9.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 5 hits.
KO	K05127.
Pfam	PF07679. I-set. 4 hits. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SMART	SM00409. IG. 2 hits. SM00408. IGc2. 3 hits. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50835. IG_LIKE. 5 hits. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	797729.

Entry information

Entry name

PTK7_DROME

Accession

Primary (citable) accession number: Q6AWJ9
Secondary accession number(s): C4IXZ4, Q24327

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project