ID   Z324B_HUMAN             Reviewed;         544 AA.
AC   Q6AW86; B2RTZ6; Q6ZMX8; Q6ZS42;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   27-MAR-2024, entry version 162.
DE   RecName: Full=Zinc finger protein 324B;
GN   Name=ZNF324B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain, and Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Endometrial adenocarcinoma;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-214, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25218447; DOI=10.1038/nsmb.2890;
RA   Hendriks I.A., D'Souza R.C., Yang B., Verlaan-de Vries M., Mann M.,
RA   Vertegaal A.C.;
RT   "Uncovering global SUMOylation signaling networks in a site-specific
RT   manner.";
RL   Nat. Struct. Mol. Biol. 21:927-936(2014).
CC   -!- FUNCTION: May be involved in transcriptional regulation.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6AW86-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6AW86-2; Sequence=VSP_023658;
CC   -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC       family. {ECO:0000305}.
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DR   EMBL; AK127750; BAC87113.1; -; mRNA.
DR   EMBL; AK131452; BAD18597.1; -; mRNA.
DR   EMBL; BX648711; CAH10556.1; -; mRNA.
DR   EMBL; BC140881; AAI40882.1; -; mRNA.
DR   EMBL; BC140882; AAI40883.1; -; mRNA.
DR   CCDS; CCDS33138.1; -. [Q6AW86-1]
DR   RefSeq; NP_997278.2; NM_207395.2. [Q6AW86-1]
DR   RefSeq; XP_005258972.1; XM_005258915.4. [Q6AW86-1]
DR   RefSeq; XP_005258975.1; XM_005258918.4. [Q6AW86-1]
DR   AlphaFoldDB; Q6AW86; -.
DR   SMR; Q6AW86; -.
DR   BioGRID; 132751; 88.
DR   ELM; Q6AW86; -.
DR   IntAct; Q6AW86; 79.
DR   STRING; 9606.ENSP00000337473; -.
DR   iPTMnet; Q6AW86; -.
DR   PhosphoSitePlus; Q6AW86; -.
DR   BioMuta; ZNF324B; -.
DR   DMDM; 74757410; -.
DR   jPOST; Q6AW86; -.
DR   MassIVE; Q6AW86; -.
DR   MaxQB; Q6AW86; -.
DR   PaxDb; 9606-ENSP00000337473; -.
DR   PeptideAtlas; Q6AW86; -.
DR   ProteomicsDB; 66190; -. [Q6AW86-1]
DR   ProteomicsDB; 66191; -. [Q6AW86-2]
DR   Pumba; Q6AW86; -.
DR   Antibodypedia; 52700; 65 antibodies from 15 providers.
DR   DNASU; 388569; -.
DR   Ensembl; ENST00000336614.9; ENSP00000337473.3; ENSG00000249471.8. [Q6AW86-1]
DR   Ensembl; ENST00000545523.5; ENSP00000438930.1; ENSG00000249471.8. [Q6AW86-1]
DR   GeneID; 388569; -.
DR   KEGG; hsa:388569; -.
DR   MANE-Select; ENST00000336614.9; ENSP00000337473.3; NM_207395.3; NP_997278.2.
DR   UCSC; uc002qsv.2; human. [Q6AW86-1]
DR   AGR; HGNC:33107; -.
DR   CTD; 388569; -.
DR   DisGeNET; 388569; -.
DR   GeneCards; ZNF324B; -.
DR   HGNC; HGNC:33107; ZNF324B.
DR   HPA; ENSG00000249471; Low tissue specificity.
DR   neXtProt; NX_Q6AW86; -.
DR   OpenTargets; ENSG00000249471; -.
DR   PharmGKB; PA162410082; -.
DR   VEuPathDB; HostDB:ENSG00000249471; -.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000163047; -.
DR   HOGENOM; CLU_002678_0_2_1; -.
DR   InParanoid; Q6AW86; -.
DR   OMA; ECPGRAS; -.
DR   OrthoDB; 5255739at2759; -.
DR   PhylomeDB; Q6AW86; -.
DR   TreeFam; TF337574; -.
DR   PathwayCommons; Q6AW86; -.
DR   Reactome; R-HSA-212436; Generic Transcription Pathway.
DR   SignaLink; Q6AW86; -.
DR   BioGRID-ORCS; 388569; 18 hits in 1176 CRISPR screens.
DR   GenomeRNAi; 388569; -.
DR   Pharos; Q6AW86; Tdark.
DR   PRO; PR:Q6AW86; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q6AW86; Protein.
DR   Bgee; ENSG00000249471; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 95 other cell types or tissues.
DR   ExpressionAtlas; Q6AW86; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd07765; KRAB_A-box; 1.
DR   Gene3D; 6.10.140.140; -; 1.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 9.
DR   InterPro; IPR001909; KRAB.
DR   InterPro; IPR036051; KRAB_dom_sf.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PANTHER; PTHR23232; KRAB DOMAIN C2H2 ZINC FINGER; 1.
DR   PANTHER; PTHR23232:SF143; ZINC FINGER PROTEIN 747; 1.
DR   Pfam; PF01352; KRAB; 1.
DR   Pfam; PF00096; zf-C2H2; 8.
DR   SMART; SM00349; KRAB; 1.
DR   SMART; SM00355; ZnF_C2H2; 9.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 5.
DR   SUPFAM; SSF109640; KRAB domain (Kruppel-associated box); 1.
DR   PROSITE; PS50805; KRAB; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 9.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 9.
DR   Genevisible; Q6AW86; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation;
KW   Ubl conjugation; Zinc; Zinc-finger.
FT   CHAIN           1..544
FT                   /note="Zinc finger protein 324B"
FT                   /id="PRO_0000280405"
FT   DOMAIN          1..72
FT                   /note="KRAB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT   ZN_FING         257..279
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         285..307
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         313..335
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         341..363
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         369..391
FT                   /note="C2H2-type 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         397..419
FT                   /note="C2H2-type 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         425..447
FT                   /note="C2H2-type 7"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         453..475
FT                   /note="C2H2-type 8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         481..503
FT                   /note="C2H2-type 9"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          184..222
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          508..544
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CROSSLNK        214
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:25218447"
FT   VAR_SEQ         1..79
FT                   /note="MTFEDVAVYFSQEEWGLLDTAQRALYRHVMLENFTLVTSLGLSTSRPRVVIQ
FT                   LERGEEPWVPSGKDMTLARNTYGRLNS -> MGVPTAVSATPVRADASSKPQPLLQSQP
FT                   HLFFFPKLLSRLLGSPLPVHSAGPGPLLTRMPQATTVSLRL (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_023658"
FT   VARIANT         63
FT                   /note="S -> G (in dbSNP:rs12611254)"
FT                   /id="VAR_052811"
FT   CONFLICT        402
FT                   /note="C -> R (in Ref. 1; BAD18597)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        457
FT                   /note="D -> G (in Ref. 1; BAC87113)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   544 AA;  60602 MW;  7CC2D443961CA98D CRC64;
     MTFEDVAVYF SQEEWGLLDT AQRALYRHVM LENFTLVTSL GLSTSRPRVV IQLERGEEPW
     VPSGKDMTLA RNTYGRLNSG SWSLTEDRDV SGEWPRAFPD TPPGMTTSVF PVADACHSVK
     SLQRQPGASP SQERKPTGVS VIYWERLLLG SRSDQASISL RLTSPLRPPK SSRPREKTFT
     EYRVPGRQPR TPERQKPCAQ EVPGRAFGNA SDLKAASGGR DRRMGAAWQE PHRLLGGQEP
     STWDELGEAL HAGEKSFECR ACSKVFVKSS DLLKHLRTHT GERPYECTQC GKAFSQTSHL
     TQHQRIHSGE TPYACPVCGK AFRHSSSLVR HQRIHTAEKS FRCSECGKAF SHGSNLSQHR
     KIHAGGRPYA CAQCGRRFCR NSHLIQHERT HTGEKPFVCA LCGAAFSQGS SLFLHQRVHT
     GEKPFACAQC GRSFSRSSNL TQHQLLHTGE RPFRCVDCGK GFAKGAVLLS HRRIHTGEKP
     FVCTQCGRAF RERPALLHHQ RIHTTEKTNA AAPDCTPGPG FLQGHHRKVR RGGKPSPVLK
     PAKV
//