Q6AW86 (Z324B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 324B

Gene names

Name:

ZNF324B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in transcriptional regulation.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 9 C2H2-type zinc fingers. Contains 1 KRAB domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6AW86-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6AW86-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MTFEDVAVYF...ARNTYGRLNS → MGVPTAVSAT...PQATTVSLRL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Zinc finger protein 324B		PRO_0000280405
Regions
	Domain	1 – 72	72	KRAB
	Zinc finger	257 – 279	23	C2H2-type 1
	Zinc finger	285 – 307	23	C2H2-type 2
	Zinc finger	313 – 335	23	C2H2-type 3
	Zinc finger	341 – 363	23	C2H2-type 4
	Zinc finger	369 – 391	23	C2H2-type 5
	Zinc finger	397 – 419	23	C2H2-type 6
	Zinc finger	425 – 447	23	C2H2-type 7
	Zinc finger	453 – 475	23	C2H2-type 8
	Zinc finger	481 – 503	23	C2H2-type 9
Natural variations
	Alternative sequence	1 – 79	79	MTFED…GRLNS → MGVPTAVSATPVRADASSKP QPLLQSQPHLFFFPKLLSRL LGSPLPVHSAGPGPLLTRMP QATTVSLRL in isoform 2.		VSP_023658
	Natural variant	63	1	S → G. Corresponds to variant rs12611254 [ dbSNP | Ensembl ].		VAR_052811
Experimental info
	Sequence conflict	402	1	C → R in BAD18597. Ref.1
	Sequence conflict	457	1	D → G in BAC87113. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 7CC2D443961CA98D	FASTA	544	60,602
	10 20 30 40 50 60 MTFEDVAVYF SQEEWGLLDT AQRALYRHVM LENFTLVTSL GLSTSRPRVV IQLERGEEPW 70 80 90 100 110 120 VPSGKDMTLA RNTYGRLNSG SWSLTEDRDV SGEWPRAFPD TPPGMTTSVF PVADACHSVK 130 140 150 160 170 180 SLQRQPGASP SQERKPTGVS VIYWERLLLG SRSDQASISL RLTSPLRPPK SSRPREKTFT 190 200 210 220 230 240 EYRVPGRQPR TPERQKPCAQ EVPGRAFGNA SDLKAASGGR DRRMGAAWQE PHRLLGGQEP 250 260 270 280 290 300 STWDELGEAL HAGEKSFECR ACSKVFVKSS DLLKHLRTHT GERPYECTQC GKAFSQTSHL 310 320 330 340 350 360 TQHQRIHSGE TPYACPVCGK AFRHSSSLVR HQRIHTAEKS FRCSECGKAF SHGSNLSQHR 370 380 390 400 410 420 KIHAGGRPYA CAQCGRRFCR NSHLIQHERT HTGEKPFVCA LCGAAFSQGS SLFLHQRVHT 430 440 450 460 470 480 GEKPFACAQC GRSFSRSSNL TQHQLLHTGE RPFRCVDCGK GFAKGAVLLS HRRIHTGEKP 490 500 510 520 530 540 FVCTQCGRAF RERPALLHHQ RIHTTEKTNA AAPDCTPGPG FLQGHHRKVR RGGKPSPVLK PAKV
	Isoform 2 [UniParc]. Checksum: 0B2458BBF618BDC9	FASTA	534	58,806
	10 20 30 40 50 60 MGVPTAVSAT PVRADASSKP QPLLQSQPHL FFFPKLLSRL LGSPLPVHSA GPGPLLTRMP 70 80 90 100 110 120 QATTVSLRLG SWSLTEDRDV SGEWPRAFPD TPPGMTTSVF PVADACHSVK SLQRQPGASP 130 140 150 160 170 180 SQERKPTGVS VIYWERLLLG SRSDQASISL RLTSPLRPPK SSRPREKTFT EYRVPGRQPR 190 200 210 220 230 240 TPERQKPCAQ EVPGRAFGNA SDLKAASGGR DRRMGAAWQE PHRLLGGQEP STWDELGEAL 250 260 270 280 290 300 HAGEKSFECR ACSKVFVKSS DLLKHLRTHT GERPYECTQC GKAFSQTSHL TQHQRIHSGE 310 320 330 340 350 360 TPYACPVCGK AFRHSSSLVR HQRIHTAEKS FRCSECGKAF SHGSNLSQHR KIHAGGRPYA 370 380 390 400 410 420 CAQCGRRFCR NSHLIQHERT HTGEKPFVCA LCGAAFSQGS SLFLHQRVHT GEKPFACAQC 430 440 450 460 470 480 GRSFSRSSNL TQHQLLHTGE RPFRCVDCGK GFAKGAVLLS HRRIHTGEKP FVCTQCGRAF 490 500 510 520 530 RERPALLHHQ RIHTTEKTNA AAPDCTPGPG FLQGHHRKVR RGGKPSPVLK PAKV

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Testis.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Endometrial adenocarcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK127750 mRNA. Translation: BAC87113.1. AK131452 mRNA. Translation: BAD18597.1. BX648711 mRNA. Translation: CAH10556.1. BC140881 mRNA. Translation: AAI40882.1. BC140882 mRNA. Translation: AAI40883.1.
IPI	IPI00410464. IPI00830046.
RefSeq	NP_997278.2. NM_207395.2.
UniGene	Hs.186970.
3D structure databases
HSSP	HSSP built from PDB template 1X6E based on UniProtKB P17028.
ProteinModelPortal	Q6AW86.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6AW86. 1 interaction.
Polymorphism databases
DMDM	74757410.
Proteomic databases
PaxDb	Q6AW86.
PRIDE	Q6AW86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336614; ENSP00000337473; ENSG00000249471. ENST00000545523; ENSP00000438930; ENSG00000249471.
GeneID	388569.
KEGG	hsa:388569.
UCSC	uc002qsv.1. human.
Organism-specific databases
CTD	388569.
GeneCards	GC19P059134.
HGNC	HGNC:33107. ZNF324B.
HPA	HPA023400.
neXtProt	NX_Q6AW86.
PharmGKB	PA162410082.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000234617.
HOVERGEN	HBG018163.
InParanoid	Q6AW86.
KO	K09228.
OMA	MTLARNT.
OrthoDB	EOG4K9BBT.
Gene expression databases
ArrayExpress	Q6AW86.
Bgee	Q6AW86.
CleanEx	HS_ZNF324B.
Genevestigator	Q6AW86.
Family and domain databases
Gene3D	3.30.160.60. 9 hits.
InterPro	IPR001909. Krueppel-associated_box. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF01352. KRAB. 1 hit. [Graphical view]
SMART	SM00349. KRAB. 1 hit. SM00355. ZnF_C2H2. 9 hits. [Graphical view]
SUPFAM	SSF109640. Krueppel-associated_box. 1 hit.
PROSITE	PS50805. KRAB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 9 hits. PS50157. ZINC_FINGER_C2H2_2. 9 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	388569.
NextBio	102176.

Entry information

Entry name

Z324B_HUMAN

Accession

Primary (citable) accession number: Q6AW86
Secondary accession number(s): B2RTZ6, Q6ZMX8, Q6ZS42

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	September 13, 2004
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.