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Protein

Cingulin-like protein 1

Gene

Cgnl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in anchoring the apical junctional complex, especially tight junctions, to actin-based cytoskeletons.1 Publication

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Cingulin-like protein 1
Alternative name(s):
Junction-associated coiled-coil protein
Gene namesi
Name:Cgnl1
Synonyms:Jacop, Kiaa1749
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1915428. Cgnl1.

Subcellular locationi

  • Cell junctiontight junction 1 Publication

  • Note: Localizes to the apical junction complex composed of tight and adherens junctions. In the liver and kidney, it is also found along non-junctional actin filament bundles in addition to the apical junction.

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • apical junction complex Source: MGI
  • bicellular tight junction Source: MGI
  • myosin complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12981298Cingulin-like protein 1PRO_0000312876Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei113 – 1131PhosphoserineBy similarity
Modified residuei203 – 2031PhosphoserineCombined sources
Modified residuei257 – 2571PhosphoserineCombined sources
Modified residuei284 – 2841PhosphoserineCombined sources
Modified residuei298 – 2981PhosphoserineCombined sources
Modified residuei299 – 2991PhosphoserineBy similarity
Modified residuei389 – 3891PhosphoserineBy similarity
Modified residuei392 – 3921PhosphoserineBy similarity
Modified residuei483 – 4831PhosphoserineBy similarity
Modified residuei679 – 6791PhosphoserineCombined sources
Modified residuei705 – 7051PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6AW69.
PaxDbiQ6AW69.
PRIDEiQ6AW69.

PTM databases

iPTMnetiQ6AW69.
PhosphoSiteiQ6AW69.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in the kidney and lung.1 Publication

Gene expression databases

CleanExiMM_CGNL1.

Interactioni

Subunit structurei

Homodimer or oligomer.Curated

Protein-protein interaction databases

BioGridi212704. 1 interaction.
STRINGi10090.ENSMUSP00000072672.

Structurei

3D structure databases

ProteinModelPortaliQ6AW69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 551551HeadAdd
BLAST
Regioni1260 – 129839TailAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili605 – 1252648Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 5115ZIMBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi297 – 3015Poly-Ser
Compositional biasi1265 – 12717Poly-Asp

Domaini

The head region is responsible for both junction and actin filament-based distribution.1 Publication

Sequence similaritiesi

Belongs to the cingulin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGSJ. Eukaryota.
ENOG410YEC7. LUCA.
HOGENOMiHOG000060190.
HOVERGENiHBG107670.
InParanoidiQ6AW69.
PhylomeDBiQ6AW69.

Family and domain databases

InterProiIPR002928. Myosin_tail.
[Graphical view]
PfamiPF01576. Myosin_tail_1. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6AW69-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELYFGEYQH VQQEYGVHLR LASGDTPKPR NSQPSKAGSY GVSIRVQGID
60 70 80 90 100
GHPYIVLNNT ERCLAGTPFP ENAPSFPSSV INNLSLHPSN GTVLKENTPE
110 120 130 140 150
ELQLPENPYL QTSPLRGQKQ FSLHEGRNGV LERKDGPTKL PHVLNFQRHP
160 170 180 190 200
ELLQPYDPEK NEVNAKKHHP PESPWLRNAT EDGTNCKKSR NCFPKSYGSQ
210 220 230 240 250
PNSPTSEDLA KTNMTAIRLC SSVVIEDPQK QTSVCVNVQR CAKEGVGEET
260 270 280 290 300
LSPRRKSPTA PSPQAYSETK KNRPDVLPFR RQDSAGPILD GARSRRSSSS
310 320 330 340 350
STTPTSATSL YKFLLDDQEC AIHADSVNRH ENRRYIPFLP GTGRDIDTCS
360 370 380 390 400
IPGVDQLIEK FDQKPGLQRR GRSGKRNRIN PDDRKRSRSV DSAFPFGLQG
410 420 430 440 450
NTEYLTEFSR NLGKSSEHLL RPSQVFPQRS VAQEHRGKHS PSSPPAKLQG
460 470 480 490 500
GAQGAHPKPP LQNKDGKVLN KGRQESTGAC APSLPAPNKK EEEIKIATAT
510 520 530 540 550
LMLQNRAVAA TSDSGAKKIS VKTFPSDSST QATPDLLKGQ QELTQQTNEE
560 570 580 590 600
TAKQILYNYL KEGGTDNEDA TKRKVNLVFE KIQTLKSRAA GSAQGSNQAP
610 620 630 640 650
NSPSEGNSLL DQKNKLILEV SELQQQLQLE MKNQQNIKEE RERMREDLEE
660 670 680 690 700
LRVRHQSQVE ETATLQRRLE ESEGELRKSL EELFQVKMER EQHQTEIRDL
710 720 730 740 750
QDQLSEMHDE LDSTKRSEDR EKGALIEELL QAKQDLQDLL IAKEEQEDLL
760 770 780 790 800
RKRERELTAL KGALKEEVSS HDQEMDKLKE QYDAELQAFR ESVEEATKNV
810 820 830 840 850
EVLASRSNSS EQSQAEADLR EKVLKEENEK LQGRIAELER RAAQLQRQME
860 870 880 890 900
DVKGDEAQAK ETLRKCESEV QQLEEALVHA RKEEKEATCA RRALEKELEQ
910 920 930 940 950
AQRELSQVSQ EQKELLEKLR DEAEQKEQLR KLKNEMESER WHLDKTIQKL
960 970 980 990 1000
QKEMADIAEA SRTSSLELQK QLGEYKEKNR RELAEMQTQL KEKCLEVEKA
1010 1020 1030 1040 1050
RLAASKMQDE LRLKEEELQD YQRAEEEALT KRQLLEQSLK DLEYELEAKS
1060 1070 1080 1090 1100
HLKDDRSRLI KQMEDKVSQL EIELEEERTN ADLLSERITW SREQMEQMRS
1110 1120 1130 1140 1150
ELLQEKAAKQ DLECDKISLE RQNKDLKSRI IHLEGSYRSS KEGLVVQMEA
1160 1170 1180 1190 1200
RIAELEDRLE NEERDRANLQ LSNRRLERKV KELVMQVDDE HLSLTDQKDQ
1210 1220 1230 1240 1250
LSLRLKAMKR QVEEAEEEID RLESSKKKLQ RELEEQMGVN EQLQGQLNSL
1260 1270 1280 1290
KKGLRLKTLS SKVLDDSDDD DLSSDAGSLY EAPLSYAFPK DSTIASQI
Length:1,298
Mass (Da):148,231
Last modified:December 4, 2007 - v2
Checksum:i535EDD1B752B3752
GO
Isoform 2 (identifier: Q6AW69-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1184: Missing.

Show »
Length:114
Mass (Da):12,912
Checksum:i46BE1873E5B9F2C8
GO
Isoform 3 (identifier: Q6AW69-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     450-450: Missing.
     728-798: Missing.

Show »
Length:1,226
Mass (Da):139,862
Checksum:i145791C242113AE7
GO
Isoform 4 (identifier: Q6AW69-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     450-450: Missing.

Show »
Length:1,297
Mass (Da):148,174
Checksum:iCF9D04DA81A38392
GO
Isoform 5 (identifier: Q6AW69-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     450-450: Missing.
     633-635: Missing.

Show »
Length:1,294
Mass (Da):147,803
Checksum:iAC576CA761302A07
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti464 – 4641K → I in BAC29612 (PubMed:16141072).Curated
Sequence conflicti529 – 5291S → G in BAD34967 (PubMed:15292197).Curated
Sequence conflicti902 – 9021Q → R in BAD32530 (PubMed:15368895).Curated
Sequence conflicti948 – 9481Q → E in BAD32530 (PubMed:15368895).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11841184Missing in isoform 2. 2 PublicationsVSP_029947Add
BLAST
Alternative sequencei450 – 4501Missing in isoform 3, isoform 4 and isoform 5. 2 PublicationsVSP_029948
Alternative sequencei633 – 6353Missing in isoform 5. 1 PublicationVSP_029949
Alternative sequencei728 – 79871Missing in isoform 3. 1 PublicationVSP_029950Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB186125 mRNA. Translation: BAD34967.1.
AK018850 mRNA. Translation: BAB31463.1.
AK036871 mRNA. Translation: BAC29612.1.
AK040774 mRNA. Translation: BAC30701.2.
BC031499 mRNA. Translation: AAH31499.1.
BC039211 mRNA. Translation: AAH39211.1.
AK173252 mRNA. Translation: BAD32530.1.
CCDSiCCDS23328.1. [Q6AW69-4]
RefSeqiNP_001291291.1. NM_001304362.1.
NP_080875.3. NM_026599.5.
UniGeneiMm.99961.

Genome annotation databases

GeneIDi68178.
KEGGimmu:68178.
UCSCiuc009qpb.2. mouse. [Q6AW69-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB186125 mRNA. Translation: BAD34967.1.
AK018850 mRNA. Translation: BAB31463.1.
AK036871 mRNA. Translation: BAC29612.1.
AK040774 mRNA. Translation: BAC30701.2.
BC031499 mRNA. Translation: AAH31499.1.
BC039211 mRNA. Translation: AAH39211.1.
AK173252 mRNA. Translation: BAD32530.1.
CCDSiCCDS23328.1. [Q6AW69-4]
RefSeqiNP_001291291.1. NM_001304362.1.
NP_080875.3. NM_026599.5.
UniGeneiMm.99961.

3D structure databases

ProteinModelPortaliQ6AW69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212704. 1 interaction.
STRINGi10090.ENSMUSP00000072672.

PTM databases

iPTMnetiQ6AW69.
PhosphoSiteiQ6AW69.

Proteomic databases

MaxQBiQ6AW69.
PaxDbiQ6AW69.
PRIDEiQ6AW69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi68178.
KEGGimmu:68178.
UCSCiuc009qpb.2. mouse. [Q6AW69-2]

Organism-specific databases

CTDi84952.
MGIiMGI:1915428. Cgnl1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IGSJ. Eukaryota.
ENOG410YEC7. LUCA.
HOGENOMiHOG000060190.
HOVERGENiHBG107670.
InParanoidiQ6AW69.
PhylomeDBiQ6AW69.

Miscellaneous databases

ChiTaRSiCgnl1. mouse.
NextBioi326616.
PROiQ6AW69.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CGNL1.

Family and domain databases

InterProiIPR002928. Myosin_tail.
[Graphical view]
PfamiPF01576. Myosin_tail_1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "JACOP, a novel plaque protein localizing at the apical junctional complex with sequence similarity to cingulin."
    Ohnishi H., Nakahara T., Furuse K., Sasaki H., Tsukita S., Furuse M.
    J. Biol. Chem. 279:46014-46022(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-640 (ISOFORM 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-641 (ISOFORM 4).
    Strain: C57BL/6J.
    Tissue: Aorta, Testis, Vagina and Vein.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 804-1298 (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor and Salivary gland.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 305-1298 (ISOFORM 3).
    Tissue: Embryonic intestine.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284 AND SER-298, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203; SER-257; SER-284 AND SER-679, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiCGNL1_MOUSE
AccessioniPrimary (citable) accession number: Q6AW69
Secondary accession number(s): Q5U5U0
, Q69ZB4, Q8BLZ5, Q8BZ26, Q9D2T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: January 20, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.