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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic

Gene

ISPH

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is essential for chloroplast development (By similarity).By similarity

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.

Cofactori

[3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster per subunit.By similarity

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (ISPH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic (DXR)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic (ISPD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic (ISPE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, chloroplastic (ISPF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin), chloroplastic (ISPG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (ISPH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151Iron-sulfur (3Fe-4S)By similarity
Binding sitei145 – 1451SubstrateBy similarity
Metal bindingi206 – 2061Iron-sulfur (3Fe-4S)By similarity
Binding sitei234 – 2341SubstrateBy similarity
Binding sitei305 – 3051SubstrateBy similarity
Metal bindingi343 – 3431Iron-sulfur (3Fe-4S)By similarity
Binding sitei434 – 4341SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (EC:1.17.7.4)
Gene namesi
Name:ISPH
Ordered Locus Names:Os03g0731900, LOC_Os03g52170
ORF Names:OSJNBa0079G12.18
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131ChloroplastSequence analysisAdd
BLAST
Chaini32 – 4594284-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplasticPRO_0000417593Add
BLAST

Proteomic databases

PaxDbiQ6AVG6.
PRIDEiQ6AVG6.

Expressioni

Gene expression databases

ExpressionAtlasiQ6AVG6. baseline and differential.
GenevisibleiQ6AVG6. OS.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os03g52170.1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni372 – 3743Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the IspH family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIDH. Eukaryota.
COG0761. LUCA.
InParanoidiQ6AVG6.
OMAiAYETRRH.
OrthoDBiEOG093608MF.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AVG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATITTQLRS ALLSPAASPS RRARRAPSSV RCDSSAASSL SASASLDADF
60 70 80 90 100
DKKQFRHNLT RSDNYNRKGF GHKKETLELM SQEYTSDVIK TLKENGNQHT
110 120 130 140 150
WGPVTVKLAE AYGFCWGVER AVQIAYEARK QFPDDRIWLT NEIIHNPTVN
160 170 180 190 200
KRLEDMGVQN IPVDAGIKDF DVVEQGDVVV LPAFGAAVEE MYTLNEKKVQ
210 220 230 240 250
IVDTTCPWVS KVWNMVEKHK KGDYTSIIHG KYSHEETVAT ASFAGTYIIV
260 270 280 290 300
KNIAEASYVC DYILGGQLDG SSSTKEEFLE KFKNAVSPGF DPDVDLVKVG
310 320 330 340 350
IANQTTMLKG ETEEIGKLVE KTMMRRFGVE NVNDHFIAFN TICDATQERQ
360 370 380 390 400
DAMYQLVKEK VDLILVVGGW NSSNTSHLQE IGELSGIPSY WIDSEQRIGP
410 420 430 440 450
GNKISYKLNH GELVEKENWL PEGPITIGVT SGASTPDKVV EDALQKVFEI

KRQEVLQAA
Length:459
Mass (Da):51,064
Last modified:September 13, 2004 - v1
Checksum:i7134DF044F1814DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC103550 Genomic DNA. Translation: AAT77894.1.
DP000009 Genomic DNA. Translation: ABF98702.1.
AP008209 Genomic DNA. Translation: BAF13081.1.
AP014959 Genomic DNA. Translation: BAS86228.1.
AK064873 mRNA. Translation: BAG89250.1.
AK121561 mRNA. Translation: BAH00550.1.
RefSeqiXP_015632597.1. XM_015777111.1.
UniGeneiOs.37894.

Genome annotation databases

EnsemblPlantsiOS03T0731900-01; OS03T0731900-01; OS03G0731900.
GeneIDi4334003.
GrameneiOS03T0731900-01; OS03T0731900-01; OS03G0731900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC103550 Genomic DNA. Translation: AAT77894.1.
DP000009 Genomic DNA. Translation: ABF98702.1.
AP008209 Genomic DNA. Translation: BAF13081.1.
AP014959 Genomic DNA. Translation: BAS86228.1.
AK064873 mRNA. Translation: BAG89250.1.
AK121561 mRNA. Translation: BAH00550.1.
RefSeqiXP_015632597.1. XM_015777111.1.
UniGeneiOs.37894.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g52170.1.

Proteomic databases

PaxDbiQ6AVG6.
PRIDEiQ6AVG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0731900-01; OS03T0731900-01; OS03G0731900.
GeneIDi4334003.
GrameneiOS03T0731900-01; OS03T0731900-01; OS03G0731900.

Phylogenomic databases

eggNOGiENOG410IIDH. Eukaryota.
COG0761. LUCA.
InParanoidiQ6AVG6.
OMAiAYETRRH.
OrthoDBiEOG093608MF.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Gene expression databases

ExpressionAtlasiQ6AVG6. baseline and differential.
GenevisibleiQ6AVG6. OS.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPH_ORYSJ
AccessioniPrimary (citable) accession number: Q6AVG6
Secondary accession number(s): A0A0P0W2J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: September 13, 2004
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.