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Protein

Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic

Gene

Os03g0617900

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ, chloroplastic (Os03g0279400), Arginine biosynthesis bifunctional protein ArgJ, chloroplastic (OsJ_10355)
  2. no protein annotated in this organism
  3. Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic (Os03g0617900)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei219 – 2191By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00068; UER00108.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic (EC:1.2.1.38)
Short name:
AGPR
Alternative name(s):
N-acetyl-glutamate semialdehyde dehydrogenase
Short name:
NAGSA dehydrogenase
Gene namesi
Ordered Locus Names:Os03g0617900, LOC_Os03g42110
ORF Names:OSJNBa0063J18.8
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7474ChloroplastSequence analysisAdd
BLAST
Chaini75 – 415341Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplasticPRO_0000247610Add
BLAST

Proteomic databases

PaxDbiQ6AV34.
PRIDEiQ6AV34.

Expressioni

Gene expression databases

ExpressionAtlasiQ6AV34. baseline and differential.
GenevisibleiQ6AV34. OS.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi39947.LOC_Os03g42110.1.

Structurei

Secondary structure

1
415
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi73 – 797Combined sources
Helixi83 – 9210Combined sources
Beta strandi96 – 1049Combined sources
Turni107 – 1104Combined sources
Helixi113 – 1164Combined sources
Helixi118 – 1203Combined sources
Helixi131 – 1333Combined sources
Helixi136 – 1383Combined sources
Beta strandi140 – 1445Combined sources
Beta strandi147 – 1493Combined sources
Helixi150 – 1567Combined sources
Beta strandi163 – 1664Combined sources
Turni170 – 1723Combined sources
Helixi176 – 1838Combined sources
Helixi190 – 1934Combined sources
Helixi201 – 21010Combined sources
Beta strandi212 – 2154Combined sources
Helixi219 – 23315Combined sources
Beta strandi239 – 2413Combined sources
Beta strandi243 – 2497Combined sources
Helixi250 – 2534Combined sources
Helixi259 – 2613Combined sources
Helixi263 – 2664Combined sources
Helixi280 – 29213Combined sources
Beta strandi299 – 30911Combined sources
Beta strandi311 – 3199Combined sources
Helixi325 – 33612Combined sources
Beta strandi342 – 3443Combined sources
Helixi353 – 3553Combined sources
Turni356 – 3583Combined sources
Beta strandi362 – 3687Combined sources
Beta strandi374 – 3818Combined sources
Turni383 – 3886Combined sources
Helixi389 – 40012Combined sources
Turni404 – 4074Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CVOX-ray2.20A/B/C/D50-415[»]
ProteinModelPortaliQ6AV34.
SMRiQ6AV34. Positions 68-415.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6AV34.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4354. Eukaryota.
COG0002. LUCA.
HOGENOMiHOG000254904.
InParanoidiQ6AV34.
KOiK00145.
OMAiTFVPHLT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00150. ArgC_type1.
InterProiIPR023013. AGPR_AS.
IPR000706. AGPR_type-1.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01850. argC. 1 hit.
PROSITEiPS01224. ARGC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AV34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSTALGGGA PARLGLAPKD GVFGSNLKQC GGFMLKTTPK VGSSSVRVRA
60 70 80 90 100
SVASSPQKQH SPKTSGVKSG EEVRIAVLGA SGYTGAEIVR LLANHPQFRI
110 120 130 140 150
KVMTADRKAG EQFGSVFPHL ITQDLPNLVA VKDADFSNVD AVFCCLPHGT
160 170 180 190 200
TQEIIKGLPQ ELKIVDLSAD FRLRDINEYA EWYGHSHRAP ELQQEAVYGL
210 220 230 240 250
TEVLRNEIRN ARLVANPGCY PTSIQLPLVP LIKAKLIKVS NIIIDAKSGV
260 270 280 290 300
SGAGRGAKEA NLYTEIAEGI HAYGIKGHRH VPEIEQGLSE AAESKVTISF
310 320 330 340 350
TPNLICMKRG MQSTMFVEMA PGVTANDLYQ HLKSTYEGEE FVKLLNGSSV
360 370 380 390 400
PHTRHVVGSN YCFMNVFEDR IPGRAIIISV IDNLVKGASG QAVQNLNLMM
410
GLPENTGLQY QPLFP
Length:415
Mass (Da):44,786
Last modified:September 13, 2004 - v1
Checksum:i7AD9E6788B065016
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC107206 Genomic DNA. Translation: AAT77050.1.
DP000009 Genomic DNA. Translation: ABF97646.1.
AP008209 Genomic DNA. Translation: BAF12582.1.
AP014959 Genomic DNA. Translation: BAS85284.1.
AK071544 mRNA. Translation: BAG92548.1.
RefSeqiXP_015632676.1. XM_015777190.1.
UniGeneiOs.16888.

Genome annotation databases

EnsemblPlantsiOS03T0617900-01; OS03T0617900-01; OS03G0617900.
GeneIDi4333458.
GrameneiOS03T0617900-01; OS03T0617900-01; OS03G0617900.
KEGGiosa:4333458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC107206 Genomic DNA. Translation: AAT77050.1.
DP000009 Genomic DNA. Translation: ABF97646.1.
AP008209 Genomic DNA. Translation: BAF12582.1.
AP014959 Genomic DNA. Translation: BAS85284.1.
AK071544 mRNA. Translation: BAG92548.1.
RefSeqiXP_015632676.1. XM_015777190.1.
UniGeneiOs.16888.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CVOX-ray2.20A/B/C/D50-415[»]
ProteinModelPortaliQ6AV34.
SMRiQ6AV34. Positions 68-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g42110.1.

Proteomic databases

PaxDbiQ6AV34.
PRIDEiQ6AV34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0617900-01; OS03T0617900-01; OS03G0617900.
GeneIDi4333458.
GrameneiOS03T0617900-01; OS03T0617900-01; OS03G0617900.
KEGGiosa:4333458.

Phylogenomic databases

eggNOGiKOG4354. Eukaryota.
COG0002. LUCA.
HOGENOMiHOG000254904.
InParanoidiQ6AV34.
KOiK00145.
OMAiTFVPHLT.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00108.

Miscellaneous databases

EvolutionaryTraceiQ6AV34.

Gene expression databases

ExpressionAtlasiQ6AV34. baseline and differential.
GenevisibleiQ6AV34. OS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00150. ArgC_type1.
InterProiIPR023013. AGPR_AS.
IPR000706. AGPR_type-1.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01850. argC. 1 hit.
PROSITEiPS01224. ARGC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Crystal structure of putative N-acetyl-gamma-glutamyl-phosphate reductase (AK071544) from rice (Oryza sativa)."
    Nonaka T., Kita A., Miura-Ohnuma J., Katoh E., Inagaki N., Yamazaki T., Miki K.
    Proteins 61:1137-1140(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 50-415, SUBUNIT.

Entry informationi

Entry nameiARGC_ORYSJ
AccessioniPrimary (citable) accession number: Q6AV34
Secondary accession number(s): Q10GQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: September 13, 2004
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.