Calcium-transporting ATPase 2, plasma membrane-type - Oryza sativa subsp. japonica (Rice)

Names and origin

Protein names

Recommended name:
    Calcium-transporting ATPase 2, plasma membrane-type
    EC=3.6.3.8
Alternative name(s):
    Ca(2+)-ATPase isoform 2

Gene names

Ordered Locus Names:	Os03g0616400, LOC_Os03g42020
ORF Names:	OJ1285_H07.3

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

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Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell, into the endoplasmic reticulum, or into organelles By similarity.
Catalytic activity	ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
Enzyme regulation	Activated by calmodulin.
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Domain	The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from electronic annotation. Source: EC calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1033

1033

Calcium-transporting ATPase 2, plasma membrane-type

PRO_0000247301

Regions

Topological domain

1 – 180

180

Cytoplasmic Potential

Transmembrane

181 – 201

21

Potential

Transmembrane

204 – 224

21

Potential

Topological domain

225 – 268

44

Cytoplasmic Potential

Transmembrane

269 – 289

21

Potential

Transmembrane

362 – 382

21

Potential

Topological domain

383 – 405

23

Cytoplasmic Potential

Transmembrane

406 – 426

21

Potential

Transmembrane

823 – 843

21

Potential

Topological domain

844 – 846

3

Cytoplasmic Potential

Transmembrane

847 – 867

21

Potential

Transmembrane

928 – 948

21

Potential

Topological domain

949 – 965

17

Cytoplasmic Potential

Transmembrane

966 – 986

21

Potential

Transmembrane

999 – 1019

21

Potential

Topological domain

1020 – 1033

14

Cytoplasmic Potential

Sites

Active site

461

1

4-aspartylphosphate intermediate By similarity

Metal binding

762

1

Magnesium By similarity

Metal binding

766

1

Magnesium By similarity

Experimental info

Sequence conflict

750

1

R → P in AK065088. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Q6ATV4-1 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 933D0F0CAB993BF6
Show »

FASTA

1,033

111,729

        10         20         30         40         50         60 
MHSGVNGCCP LRLPAAAAVH GRRIPPLLPP RGAWPGCIAA PALHRKPGRG GGGALSSCRR 

        70         80         90        100        110        120 
ASHHEKLQVA ALPSKATLEF EHGVSLRSAY IVPEDVQAAG FQIDADELAS IVESRDTKKL 

       130        140        150        160        170        180 
TVHGQLNGIA DKLGTSLTNG IVTDKDLLNQ RQDIYGVNKF AETEIRSFWE FVWEALEDTT 

       190        200        210        220        230        240 
LIILSACAIF SLVVGITTEG WPQGAHDGVG IVASILLVVS VTGTSNYQQS LQFRDLDKEK 

       250        260        270        280        290        300 
RKILVQVTRN GLRQRVLIDD LLPGDAVHLA VGDQVPADGL FISGFSVLVD ESSLTGESEP 

       310        320        330        340        350        360 
VFVNEDNPYL LSGTKVLDGS CKMLVTAVGM RTQWGKLMAV LTDGGDDETP LQTRLNGVAN 

       370        380        390        400        410        420 
TIGKIGLFFA VLTFIVLSQG IIGQKYLDGL LLSWSGDDVL EILDHFAVAV TIVVVAVPEG 

       430        440        450        460        470        480 
LPLAVTLSLA FAMKKMMNDK ALVRQLAACE TMGSATVICS DKTGTLTTNR MTVVKACICG 

       490        500        510        520        530        540 
NTIQVNNPQT PNMSSNFPEV AVETLLESIF NNTSGEVVTN QDGKYQILGT PTETALLEFA 

       550        560        570        580        590        600 
LLLDGDCKEK QLGSKIVKVE PFNSTKKRMS TILELPGGGY RAHCKGASEI VLAACDKFID 

       610        620        630        640        650        660 
ERGCIVPLDD KTSSKLNDII KAFSSEALRT LCLAYREMEE GFSTQEQIPL QGYTCIGIVG 

       670        680        690        700        710        720 
IKDPVRPGVR QSVATCRSAG ISVRMITGDN IDTAKAIARE CGILTKDGIA IEGAEFREKS 

       730        740        750        760        770        780 
AEELHDLIPK MQVLARSSPL DKHTLVKHLR TAFNEVVAVT GDGTNDAPAL READIGLAMG 

       790        800        810        820        830        840 
IAGTEVAKES ADVVILDDNF STIVTVAKWG RSVYVNIQKF VQFQLTVNVV ALLVNFTSAC 

       850        860        870        880        890        900 
FTGDAPLTAV QLLWVNMIMD TLGALALATE PPNNNLMKKA PVGRKGKFIT NVMWRNIVGQ 

       910        920        930        940        950        960 
SLYQFAVMWY LQTQGKHLFG LEGYHADIVL NTIIFNTFVF CQVFNEISSR EMEDINVLRG 

       970        980        990       1000       1010       1020 
MAGNSIFLGV LTGTIFFQFI LVQFLGDFAN TTPLTQQQWL ISILFGFLGM PIAAAIKLIA 

      1030 
VEPHEKADTR RTP

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References

[1]

"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.

Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.

[2]

"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed: 12869764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

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Cross-references

Sequence databases
	AC135557 Genomic DNA. Translation: AAT81659.1. AK065088 mRNA. No translation available.
RefSeq	NP_001050661.1.
UniGene	Os.24860
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4333451.
KEGG	osa:4333451.
Organism-specific databases
Gramene	Q6ATV4.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR006408. ATPase_P-typ_Ca-transp_PMCA. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR000695. ATPase_P-typ_H-transp. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ACA2_ORYSJ

Accession

Primary (citable) accession number: Q6ATV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	September 13, 2004
Last modified:	October 13, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Family and domain databases

Entry information

Relevant documents