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Q6ASD8 (DDL_DESPS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:DP0058
OrganismDesulfotalea psychrophila (strain LSv54 / DSM 12343) [Complete proteome] [HAMAP]
Taxonomic identifier177439 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341087

Regions

Domain108 – 311204ATP-grasp
Nucleotide binding136 – 19257ATP By similarity

Sites

Metal binding2651Magnesium or manganese 1 By similarity
Metal binding2781Magnesium or manganese 1 By similarity
Metal binding2781Magnesium or manganese 2 By similarity
Metal binding2801Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6ASD8 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: A950D8909183F348

FASTA32234,868
        10         20         30         40         50         60 
MQEQRAERLR IALIAGGTSG EREVSLTGAD GVERILDKEK YLVSRYDSAT DLPRLAADAA 

        70         80         90        100        110        120 
SIDFAFILLH GLHGEDGTIQ GFLDLLGIPY QGSGVLGSAL AMDKDLAKEF YYNAELPVAD 

       130        140        150        160        170        180 
WHTIAAGDFF YSEELIEDLG LPLVVKPACA GSSIGISLAH TEEELLAGIN HARDCSAGAI 

       190        200        210        220        230        240 
MVEQFIKGRE LTCAVLGNDD LQALPVLEIV PGDKYAFFDY EAKYQPGASE EICPALIADA 

       250        260        270        280        290        300 
LREQVQDHAI RAHQALRLRG YSRTDFIYGE DGKLYLLETN TIPGMTETSI LPQEAAATGM 

       310        320 
DFPSLLDTLI ELGLEKSKGK KG 

« Hide

References

[1]"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments."
Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P.
Environ. Microbiol. 6:887-902(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LSv54 / DSM 12343.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR522870 Genomic DNA. Translation: CAG34787.1.
RefSeqYP_063794.1. NC_006138.1.

3D structure databases

ProteinModelPortalQ6ASD8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING177439.DP0058.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG34787; CAG34787; DP0058.
GeneID2944292.
KEGGdps:DP0058.
PATRIC21709599. VBIDesPsy67261_0057.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMALCRWIVE.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycDPSY177439:GJW5-58-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_DESPS
AccessionPrimary (citable) accession number: Q6ASD8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 13, 2004
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways