Reviewed, UniProtKB/Swiss-Prot Q6AS41 (PYRC_DESPS)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	DP0105

Organism

Desulfotalea psychrophila [Complete proteome] [HAMAP]

Taxonomic identifier

84980 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfobacterales › Desulfobulbaceae › Desulfotalea

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Dihydroorotase		PRO_1000024081
Sites
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 1; via carbamate group By similarity
	Metal binding	140	1	Zinc 2; via carbamate group By similarity
	Metal binding	177	1	Zinc 2 By similarity
	Metal binding	230	1	Zinc 2 By similarity
	Metal binding	303	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	140	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AS41-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: A7319DA9FABAF02D	FASTA	421	44,919
	10 20 30 40 50 60 MIIILKGGRV IDPRNRRDED ADIWMEDGQI IAPQETVPED AKIYDLAGKW VVPGLIDMHV 70 80 90 100 110 120 HLRDPGEEYK ESIVTGTDAA AAGGFTAVAC MPNTDPVNDT GSVTRYILEK SEGAAARVYP 130 140 150 160 170 180 VGALSEGLKG ESLAEYADMK HAGAVALTDD GNPVVSSQLM RRAMEYAKSH DMLVISHSEE 190 200 210 220 230 240 PTLSKRGCMN EGLVATRLGL KGIPAVAESI MVQREIALAG LTGARLHIAH VSCIESLAAI 250 260 270 280 290 300 RDAKKRGLPV TAETAPHYFS LTDDAVIGYD THAKMNPPLR TEEHRQAIRQ ALADGTLDVI 310 320 330 340 350 360 ATDHAPHSIV EKEVTFEYAA NGIVGLETSL GLSLALVRDG VLTPSRLVEL MSSVPAQILS 370 380 390 400 410 420 VAGGELGVGA IADVTVIDPE LEHTFLIAEG RSRGANSPFD GWKLKGAATL TFVAGEESYC R

References

[1]

"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments." Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P. Environ. Microbiol. 6:887-902(2004) [PubMed: 15305914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LSv54 / DSM 12343.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR522870 Genomic DNA. Translation: CAG34834.1.
RefSeq	YP_063841.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2945698.
GenomeReviews	Gene locus DP0105 in contig CR522870_GR.
KEGG	dps:DP0105.
NMPDR	fig|177439.1.peg.105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6AS41.
Enzyme and pathway databases
BioCyc	DPSY177439:DP0105-MON.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_DESPS

Accession

Primary (citable) accession number: Q6AS41

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2004
Last modified:	September 2, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)