Reviewed, UniProtKB/Swiss-Prot Q6ARX8 (QUEF_DESPS)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	DP0168

Organism

Desulfotalea psychrophila [Complete proteome] [HAMAP]

Taxonomic identifier

84980 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfobacterales › Desulfobulbaceae › Desulfotalea

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000163029

Sequences

	Sequence		Length	Mass (Da)
	Q6ARX8-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: C2382C09C9CB89EB	FASTA	276	31,313
	10 20 30 40 50 60 MTENINAGIP LGKQVGHSET YDPSHLFPVA RREARKSLGL ADDLPFSGPD IWNAYELSWL 70 80 90 100 110 120 DSKGKPCVAL GEITFPCTSE NIIESKSLKL YLNSFNLTRF TSTARVKEII REDLSRVAGA 130 140 150 160 170 180 EVEVKILTPE EFTEVTISAP EGSCIDDLEL EEEINAYRPT ANYLSTGPEE TEEELYTNLL 190 200 210 220 230 240 RTNCPVTGQP DWATVIINYR GKAIDQRGLL RYIISFRQHE GFHENCVERI FMDILNRCAP 250 260 270 ARLTVYARFT RRGGLDINPY RTTHAEHFVN LRLARQ

References

[1]

"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments." Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P. Environ. Microbiol. 6:887-902(2004) [PubMed: 15305914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LSv54 / DSM 12343.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR522870 Genomic DNA. Translation: CAG34897.1.
RefSeq	YP_063904.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2945252.
GenomeReviews	Gene locus DP0168 in contig CR522870_GR.
KEGG	dps:DP0168.
NMPDR	fig|177439.1.peg.168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6ARX8.
OMA	Q6ARX8. EHNEFHE.
Enzyme and pathway databases
BioCyc	DPSY177439:DP0168-MON.
BRENDA	1.7.1.13. 280712.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR001474. GTP_CycHdrlase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_DESPS

Accession

Primary (citable) accession number: Q6ARX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2004
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)