Q6AQW7 (MTNN_DESPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Short name=MTA/SAH nucleosidase
Short name=MTAN
EC=3.2.2.9

Alternative name(s):
5'-methylthioadenosine nucleosidase
Short name=MTA nucleosidase
S-adenosylhomocysteine nucleosidase
Short name=AdoHcy nucleosidase
Short name=SAH nucleosidase
Short name=SRH nucleosidase

Gene names

Name:	mtnN
Ordered Locus Names:	DP0528

Organism

Desulfotalea psychrophila [Complete proteome] [HAMAP]

Taxonomic identifier

84980 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfobacterales › Desulfobulbaceae › Desulfotalea

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684 S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.
Sequence caution	The sequence CAG35257.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: EC methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684		PRO_0000359288
Regions
	Region	173 – 174	2	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Binding site	78	1	Substrate; via amide nitrogen By similarity
	Binding site	152	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	197	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AQW7 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 23D7DA6E2A8D6244	FASTA	234	25,783
	10 20 30 40 50 60 MKVGIIAAME EELTLLVDRL DNCEEVQLGH CKYYTGQING VEVGLMRCGI GKVNAAIGTT 70 80 90 100 110 120 LMIDKLKPKC LINTGVAGGF INEMNVGDIV ISSSVRHHDA DATAFGYELG QIPDMPSEFQ 130 140 150 160 170 180 ADRRLVEMAT KVNLKSKARI FEGPVFSGDS FIHTTEQVEN ILKNFPQIMA VEMEGASIAQ 190 200 210 220 230 TSHLFNIPFV LIRSISDKVR ETKSADTYTQ SMEESAKNSV QVVFEMVEQL KEGM

References

[1]

"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments." Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P. Environ. Microbiol. 6:887-902(2004) [PubMed: 15305914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LSv54 / DSM 12343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR522870 Genomic DNA. Translation: CAG35257.1. Different initiation.
RefSeq	YP_064264.1. NC_006138.1.
3D structure databases
HSSP	HSSP built from PDB template 1JYS based on UniProtKB P0AF12.
ProteinModelPortal	Q6AQW7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2946735.
GenomeReviews	Gene locus DP0528 in contig CR522870_GR.
KEGG	dps:DP0528.
NMPDR	fig|177439.1.peg.528.
PATRIC	21710633. VBIDesPsy67261_0569.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG367723.
OMA	LEHFPTM.
ProtClustDB	CLSK2523297.
Enzyme and pathway databases
BioCyc	DPSY177439:DP0528-MONOMER.
Family and domain databases
HAMAP	MF_01684. Salvage_tnN. [Tree]
InterPro	IPR010049. MTA_SAH_Nsdase. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
KO	K01243.
PANTHER	PTHR21234. PNP_UDP. 1 hit. PTHR21234:SF6. PTHR21234:SF6. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNN_DESPS

Accession

Primary (citable) accession number: Q6AQW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	January 25, 2012
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program