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Q6ANW2

- GSHAB_DESPS

UniProt

Q6ANW2 - GSHAB_DESPS

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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene
gshAB, gshF, DP1233
Organism
Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi719 – 7191Magnesium or manganese 1 By similarity
Metal bindingi738 – 7381Magnesium or manganese 1 By similarity
Metal bindingi738 – 7381Magnesium or manganese 2 By similarity
Metal bindingi740 – 7401Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi539 – 59759ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDPSY177439:GJW5-1272-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name:
GCS-GS
Including the following 2 domains:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GS
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshAB
Synonyms:gshF
Ordered Locus Names:DP1233
OrganismiDesulfotalea psychrophila (strain LSv54 / DSM 12343)
Taxonomic identifieri177439 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea
ProteomesiUP000000602: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Glutathione biosynthesis bifunctional protein GshABUniRule annotationPRO_0000192550Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi177439.DP1233.

Structurei

3D structure databases

ProteinModelPortaliQ6ANW2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini512 – 768257ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 346346Glutamate--cysteine ligaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiHVEYVKN.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
PF08443. RimK. 1 hit.
[Graphical view]
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6ANW2-1 [UniParc]FASTAAdd to Basket

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MAFSKNILDS LPPLISKQIF EGFFGFEKEN IRVDSRGKLA LTPHPRELGE    50
KTSHPYITTD FSESQIEIIT PPLPSIAESL GFLETLHDLV SIELKDEYLW 100
PQSAPPILPE REEDIPIAHF GGEFREQEEY RLQLAKIYGR KRQMFSGIHF 150
NISLPERFLE LLHEEGKQEQ PFAEFREDIY MKTVRNFLRH RWFLICLLGA 200
SPVIHKSYRK HCIDMLSPFA KDAYHFPYAT SIRNNICGYR NTQDFHLNYS 250
TLTDYRESLQ ELVEKKVLRD IRENYAPIRI KTTTDPKRIN HLEIRLLDLN 300
PFFKTGVNPL HAEIIHIFLI YCLLCPEETS FTSKEQETAN RNQEQAATEG 350
LNPGAIICDA DGNEQRLDKQ LAHCLQEIQQ TVSPHLPPEY RAGMEELERL 400
VQNQASRPTD TLLKEIKQEG FTEWHMKQAL KFLKKSHDEQ FIFHGLRDME 450
LSTQLLLRRA ALRGVSFEIM DRQENFVCLE QAGKREYVMQ ASRTSLDNYI 500
SVLSMENKVI TKKILDQAGI NTPKGRSYSS PSEALADYPY YRGRAIVIKP 550
KSTNFGIGIT IIKENNRHDF FAQGIAQAFK HEATVLIENF SSGKEYRFFI 600
VNDQVVGILH RVPANVTGDG TSSVQVLVTE KNKNPLRGRG YRTPLEKIKL 650
EETEEMFLAS QGYSFATVPA KDQRIYLREN SNISTGGDSI DFTDKVPQSY 700
KDIAVRAAQA LQVKITGLDM MIDSLEEDAA EDNFSIIELN FNPAIHIHCH 750
PYIGKNRHLD DKILDALGFT GAEEAGEKA 779
Length:779
Mass (Da):89,336
Last modified:September 13, 2004 - v1
Checksum:i1985FBD93F20889E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR522870 Genomic DNA. Translation: CAG35962.1.
RefSeqiYP_064969.1. NC_006138.1.

Genome annotation databases

EnsemblBacteriaiCAG35962; CAG35962; DP1233.
GeneIDi2944854.
KEGGidps:DP1233.
PATRICi21712227. VBIDesPsy67261_1331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR522870 Genomic DNA. Translation: CAG35962.1 .
RefSeqi YP_064969.1. NC_006138.1.

3D structure databases

ProteinModelPortali Q6ANW2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 177439.DP1233.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG35962 ; CAG35962 ; DP1233 .
GeneIDi 2944854.
KEGGi dps:DP1233.
PATRICi 21712227. VBIDesPsy67261_1331.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OMAi HVEYVKN.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci DPSY177439:GJW5-1272-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
PF08443. RimK. 1 hit.
[Graphical view ]
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LSv54 / DSM 12343.

Entry informationi

Entry nameiGSHAB_DESPS
AccessioniPrimary (citable) accession number: Q6ANW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: September 13, 2004
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3