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Q6ANW2

- GSHAB_DESPS

UniProt

Q6ANW2 - GSHAB_DESPS

Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi719 – 7191Magnesium or manganese 1UniRule annotation
    Metal bindingi738 – 7381Magnesium or manganese 1UniRule annotation
    Metal bindingi738 – 7381Magnesium or manganese 2UniRule annotation
    Metal bindingi740 – 7401Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi539 – 59759ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
    3. glutathione synthase activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciDPSY177439:GJW5-1272-MONOMER.
    UniPathwayiUPA00142; UER00209.
    UPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione biosynthesis bifunctional protein GshABUniRule annotation
    Alternative name(s):
    Gamma-GCS-GSUniRule annotation
    Short name:
    GCS-GSUniRule annotation
    Including the following 2 domains:
    Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
    Alternative name(s):
    Gamma-ECSUniRule annotation
    Short name:
    GCSUniRule annotation
    Gamma-glutamylcysteine synthetaseUniRule annotation
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshABUniRule annotation
    Synonyms:gshFUniRule annotation
    Ordered Locus Names:DP1233
    OrganismiDesulfotalea psychrophila (strain LSv54 / DSM 12343)
    Taxonomic identifieri177439 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea
    ProteomesiUP000000602: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 779779Glutathione biosynthesis bifunctional protein GshABPRO_0000192550Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi177439.DP1233.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6ANW2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini512 – 768257ATP-graspUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 346346Glutamate--cysteine ligaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1181.
    HOGENOMiHOG000156471.
    KOiK01919.
    OMAiHVEYVKN.
    OrthoDBiEOG6BKJ7H.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 3 hits.
    HAMAPiMF_00782. Glut_biosynth.
    InterProiIPR011761. ATP-grasp.
    IPR013651. ATP-grasp_RimK-type.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view]
    PfamiPF04262. Glu_cys_ligase. 1 hit.
    PF08443. RimK. 1 hit.
    [Graphical view]
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6ANW2-1 [UniParc]FASTAAdd to Basket

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    MAFSKNILDS LPPLISKQIF EGFFGFEKEN IRVDSRGKLA LTPHPRELGE    50
    KTSHPYITTD FSESQIEIIT PPLPSIAESL GFLETLHDLV SIELKDEYLW 100
    PQSAPPILPE REEDIPIAHF GGEFREQEEY RLQLAKIYGR KRQMFSGIHF 150
    NISLPERFLE LLHEEGKQEQ PFAEFREDIY MKTVRNFLRH RWFLICLLGA 200
    SPVIHKSYRK HCIDMLSPFA KDAYHFPYAT SIRNNICGYR NTQDFHLNYS 250
    TLTDYRESLQ ELVEKKVLRD IRENYAPIRI KTTTDPKRIN HLEIRLLDLN 300
    PFFKTGVNPL HAEIIHIFLI YCLLCPEETS FTSKEQETAN RNQEQAATEG 350
    LNPGAIICDA DGNEQRLDKQ LAHCLQEIQQ TVSPHLPPEY RAGMEELERL 400
    VQNQASRPTD TLLKEIKQEG FTEWHMKQAL KFLKKSHDEQ FIFHGLRDME 450
    LSTQLLLRRA ALRGVSFEIM DRQENFVCLE QAGKREYVMQ ASRTSLDNYI 500
    SVLSMENKVI TKKILDQAGI NTPKGRSYSS PSEALADYPY YRGRAIVIKP 550
    KSTNFGIGIT IIKENNRHDF FAQGIAQAFK HEATVLIENF SSGKEYRFFI 600
    VNDQVVGILH RVPANVTGDG TSSVQVLVTE KNKNPLRGRG YRTPLEKIKL 650
    EETEEMFLAS QGYSFATVPA KDQRIYLREN SNISTGGDSI DFTDKVPQSY 700
    KDIAVRAAQA LQVKITGLDM MIDSLEEDAA EDNFSIIELN FNPAIHIHCH 750
    PYIGKNRHLD DKILDALGFT GAEEAGEKA 779
    Length:779
    Mass (Da):89,336
    Last modified:September 13, 2004 - v1
    Checksum:i1985FBD93F20889E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR522870 Genomic DNA. Translation: CAG35962.1.
    RefSeqiYP_064969.1. NC_006138.1.

    Genome annotation databases

    EnsemblBacteriaiCAG35962; CAG35962; DP1233.
    GeneIDi2944854.
    KEGGidps:DP1233.
    PATRICi21712227. VBIDesPsy67261_1331.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR522870 Genomic DNA. Translation: CAG35962.1 .
    RefSeqi YP_064969.1. NC_006138.1.

    3D structure databases

    ProteinModelPortali Q6ANW2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 177439.DP1233.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG35962 ; CAG35962 ; DP1233 .
    GeneIDi 2944854.
    KEGGi dps:DP1233.
    PATRICi 21712227. VBIDesPsy67261_1331.

    Phylogenomic databases

    eggNOGi COG1181.
    HOGENOMi HOG000156471.
    KOi K01919.
    OMAi HVEYVKN.
    OrthoDBi EOG6BKJ7H.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00209 .
    UPA00142 ; UER00210 .
    BioCyci DPSY177439:GJW5-1272-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 3 hits.
    HAMAPi MF_00782. Glut_biosynth.
    InterProi IPR011761. ATP-grasp.
    IPR013651. ATP-grasp_RimK-type.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view ]
    Pfami PF04262. Glu_cys_ligase. 1 hit.
    PF08443. RimK. 1 hit.
    [Graphical view ]
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LSv54 / DSM 12343.

    Entry informationi

    Entry nameiGSHAB_DESPS
    AccessioniPrimary (citable) accession number: Q6ANW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3