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Q6ANW2

- GSHAB_DESPS

UniProt

Q6ANW2 - GSHAB_DESPS

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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi719 – 7191Magnesium or manganese 1UniRule annotation
Metal bindingi738 – 7381Magnesium or manganese 1UniRule annotation
Metal bindingi738 – 7381Magnesium or manganese 2UniRule annotation
Metal bindingi740 – 7401Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi539 – 59759ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDPSY177439:GJW5-1272-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:DP1233
OrganismiDesulfotalea psychrophila (strain LSv54 / DSM 12343)
Taxonomic identifieri177439 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea
ProteomesiUP000000602: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Glutathione biosynthesis bifunctional protein GshABPRO_0000192550Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi177439.DP1233.

Structurei

3D structure databases

ProteinModelPortaliQ6ANW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini512 – 768257ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 346346Glutamate--cysteine ligaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiHVEYVKN.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
PF08443. RimK. 1 hit.
[Graphical view]
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6ANW2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFSKNILDS LPPLISKQIF EGFFGFEKEN IRVDSRGKLA LTPHPRELGE
60 70 80 90 100
KTSHPYITTD FSESQIEIIT PPLPSIAESL GFLETLHDLV SIELKDEYLW
110 120 130 140 150
PQSAPPILPE REEDIPIAHF GGEFREQEEY RLQLAKIYGR KRQMFSGIHF
160 170 180 190 200
NISLPERFLE LLHEEGKQEQ PFAEFREDIY MKTVRNFLRH RWFLICLLGA
210 220 230 240 250
SPVIHKSYRK HCIDMLSPFA KDAYHFPYAT SIRNNICGYR NTQDFHLNYS
260 270 280 290 300
TLTDYRESLQ ELVEKKVLRD IRENYAPIRI KTTTDPKRIN HLEIRLLDLN
310 320 330 340 350
PFFKTGVNPL HAEIIHIFLI YCLLCPEETS FTSKEQETAN RNQEQAATEG
360 370 380 390 400
LNPGAIICDA DGNEQRLDKQ LAHCLQEIQQ TVSPHLPPEY RAGMEELERL
410 420 430 440 450
VQNQASRPTD TLLKEIKQEG FTEWHMKQAL KFLKKSHDEQ FIFHGLRDME
460 470 480 490 500
LSTQLLLRRA ALRGVSFEIM DRQENFVCLE QAGKREYVMQ ASRTSLDNYI
510 520 530 540 550
SVLSMENKVI TKKILDQAGI NTPKGRSYSS PSEALADYPY YRGRAIVIKP
560 570 580 590 600
KSTNFGIGIT IIKENNRHDF FAQGIAQAFK HEATVLIENF SSGKEYRFFI
610 620 630 640 650
VNDQVVGILH RVPANVTGDG TSSVQVLVTE KNKNPLRGRG YRTPLEKIKL
660 670 680 690 700
EETEEMFLAS QGYSFATVPA KDQRIYLREN SNISTGGDSI DFTDKVPQSY
710 720 730 740 750
KDIAVRAAQA LQVKITGLDM MIDSLEEDAA EDNFSIIELN FNPAIHIHCH
760 770
PYIGKNRHLD DKILDALGFT GAEEAGEKA
Length:779
Mass (Da):89,336
Last modified:September 13, 2004 - v1
Checksum:i1985FBD93F20889E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR522870 Genomic DNA. Translation: CAG35962.1.
RefSeqiYP_064969.1. NC_006138.1.

Genome annotation databases

EnsemblBacteriaiCAG35962; CAG35962; DP1233.
GeneIDi2944854.
KEGGidps:DP1233.
PATRICi21712227. VBIDesPsy67261_1331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR522870 Genomic DNA. Translation: CAG35962.1 .
RefSeqi YP_064969.1. NC_006138.1.

3D structure databases

ProteinModelPortali Q6ANW2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 177439.DP1233.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG35962 ; CAG35962 ; DP1233 .
GeneIDi 2944854.
KEGGi dps:DP1233.
PATRICi 21712227. VBIDesPsy67261_1331.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OMAi HVEYVKN.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci DPSY177439:GJW5-1272-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
PF08443. RimK. 1 hit.
[Graphical view ]
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LSv54 / DSM 12343.

Entry informationi

Entry nameiGSHAB_DESPS
AccessioniPrimary (citable) accession number: Q6ANW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: September 13, 2004
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3