Reviewed, UniProtKB/Swiss-Prot Q6ANL4 (SYN_DESPS)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	DP1331

Organism

Desulfotalea psychrophila [Complete proteome] [HAMAP]

Taxonomic identifier

84980 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfobacterales › Desulfobulbaceae › Desulfotalea

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176404

Sequences

	Sequence		Length	Mass (Da)
	Q6ANL4-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 9A656B8E8DA61E72	FASTA	449	50,214
	10 20 30 40 50 60 MRQRITELLV AKAEEQVVTV AGWIRTRRDT GDFSFLEVND GSTLINMQVI ADKSLGNYEE 70 80 90 100 110 120 EVKKLSTGCS VMVEGVLKES PAKGQSVEVH ASSVTVVGWA DPETYPLQKK RHSLEFLREI 130 140 150 160 170 180 SHLRPRTNAI SAVARVRSRL SYAVHNFFQE KGFTQVHTPI ITTSDCEGAG EMFQVAATGK 190 200 210 220 230 240 DGHFFGAPAG LTVSGQLQAE VYATALGDVY TFGPTFRAEN SNTSRHLAEF WMIEPEMAFC 250 260 270 280 290 300 DLQGDMEVAE EMLKYVLADV LEHCVTDMNL FDKFISKGII ERLQSVLAHD FARVTYTEAV 310 320 330 340 350 360 DQLLASGQKF DFAVKWGIDL QSEHERYLTE QVYKRPLIVT DYPAAIKPFY MRMNEDGKTV 370 380 390 400 410 420 AAMDILVPGI GELVGGSQRE ERYDLLAERM EAAGLDLEEY GWYLDLRKYG TVPHAGFGLG 430 440 FERLVQFVTG MANIREVIPF PRTPGFAPC

References

[1]

"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments." Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P. Environ. Microbiol. 6:887-902(2004) [PubMed: 15305914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LSv54 / DSM 12343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR522870 Genomic DNA. Translation: CAG36060.1.
RefSeq	YP_065067.1.
3D structure databases
SMR	Q6ANL4. Positions 10-446.
ModBase	Search...
Genome annotation databases
GeneID	2945640.
GenomeReviews	Gene locus DP1331 in contig CR522870_GR.
KEGG	dps:DP1331.
NMPDR	fig|177439.1.peg.1331.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	DPSY177439:DP1331-MONOMER.
BRENDA	6.1.1.22. 280712.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_DESPS

Accession

Primary (citable) accession number: Q6ANL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	September 13, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)