Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6AK48 (BIOB_DESPS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:DP2549
OrganismDesulfotalea psychrophila (strain LSv54 / DSM 12343) [Complete proteome] [HAMAP]
Taxonomic identifier177439 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Biotin synthase HAMAP-Rule MF_01694
PRO_0000381349

Sites

Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1191Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1511Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2111Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2811Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6AK48 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 2CFE820C133F9663

FASTA33636,564
        10         20         30         40         50         60 
MYRGLFLISE AVISRIEKKV LSGGKILFAE ACDLALHCAR ADLDRLLALA RQVGSLHHGK 

        70         80         90        100        110        120 
SIDLCSIVNA RSGRCSEDCK FCAQSSHYST GVDSYALVDP DYVLALAKQN EAYGVSRFSL 

       130        140        150        160        170        180 
VTAGRAVSPA FLREVASIYR RLGEETSLAP CASMGLLEPE TAEMLVEMGV RRYHCNLETS 

       190        200        210        220        230        240 
ASFFSEVCTS HTWQDKVATI EHARRAGLEV CSGGIIGLGE SMEQRIELAF ELRDLEVLSI 

       250        260        270        280        290        300 
PLNILNPIAN TPFADLPALT EREILITFAL FRLINPRAIV RTAGGRNLLA GQQKKLFLAG 

       310        320        330 
ANGAIVGDYL TTSGDGLKKD IDMFLNLGFS IGSREK 

« Hide

References

[1]"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments."
Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P.
Environ. Microbiol. 6:887-902(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LSv54 / DSM 12343.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR522870 Genomic DNA. Translation: CAG37278.1.
RefSeqYP_066285.1. NC_006138.1.

3D structure databases

ProteinModelPortalQ6AK48.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING177439.DP2549.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG37278; CAG37278; DP2549.
GeneID2945476.
KEGGdps:DP2549.
PATRIC21715202. VBIDesPsy67261_2777.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycDPSY177439:GJW5-2630-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_DESPS
AccessionPrimary (citable) accession number: Q6AK48
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 13, 2004
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways