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Q6AK48

- BIOB_DESPS

UniProt

Q6AK48 - BIOB_DESPS

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Protein

Biotin synthase

Gene

bioB

Organism
Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi119 – 1191Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi151 – 1511Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi211 – 2111Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi281 – 2811Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciDPSY177439:GJW5-2630-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:DP2549
OrganismiDesulfotalea psychrophila (strain LSv54 / DSM 12343)
Taxonomic identifieri177439 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea
ProteomesiUP000000602: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Biotin synthasePRO_0000381349Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi177439.DP2549.

Structurei

3D structure databases

ProteinModelPortaliQ6AK48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiFEIANEC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AK48-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYRGLFLISE AVISRIEKKV LSGGKILFAE ACDLALHCAR ADLDRLLALA
60 70 80 90 100
RQVGSLHHGK SIDLCSIVNA RSGRCSEDCK FCAQSSHYST GVDSYALVDP
110 120 130 140 150
DYVLALAKQN EAYGVSRFSL VTAGRAVSPA FLREVASIYR RLGEETSLAP
160 170 180 190 200
CASMGLLEPE TAEMLVEMGV RRYHCNLETS ASFFSEVCTS HTWQDKVATI
210 220 230 240 250
EHARRAGLEV CSGGIIGLGE SMEQRIELAF ELRDLEVLSI PLNILNPIAN
260 270 280 290 300
TPFADLPALT EREILITFAL FRLINPRAIV RTAGGRNLLA GQQKKLFLAG
310 320 330
ANGAIVGDYL TTSGDGLKKD IDMFLNLGFS IGSREK
Length:336
Mass (Da):36,564
Last modified:September 13, 2004 - v1
Checksum:i2CFE820C133F9663
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR522870 Genomic DNA. Translation: CAG37278.1.
RefSeqiYP_066285.1. NC_006138.1.

Genome annotation databases

EnsemblBacteriaiCAG37278; CAG37278; DP2549.
GeneIDi2945476.
KEGGidps:DP2549.
PATRICi21715202. VBIDesPsy67261_2777.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR522870 Genomic DNA. Translation: CAG37278.1 .
RefSeqi YP_066285.1. NC_006138.1.

3D structure databases

ProteinModelPortali Q6AK48.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 177439.DP2549.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG37278 ; CAG37278 ; DP2549 .
GeneIDi 2945476.
KEGGi dps:DP2549.
PATRICi 21715202. VBIDesPsy67261_2777.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi FEIANEC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci DPSY177439:GJW5-2630-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LSv54 / DSM 12343.

Entry informationi

Entry nameiBIOB_DESPS
AccessioniPrimary (citable) accession number: Q6AK48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 13, 2004
Last modified: October 1, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3