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Q6AK45

- SYI_DESPS

UniProt

Q6AK45 - SYI_DESPS

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDPSY177439:GJW5-2633-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:DP2552
    OrganismiDesulfotalea psychrophila (strain LSv54 / DSM 12343)
    Taxonomic identifieri177439 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea
    ProteomesiUP000000602: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 939939Isoleucine--tRNA ligasePRO_0000098379Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi177439.DP2552.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6AK45.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6AK45-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYRDSLNLP KTNFKMKANL AQREPMILKR WEKEGLYQML QERAQDRPLF    50
    VLHDGPPYAN GHIHLGHAFN KILKDIILRS KRASGFNAPY VPGWDCHGLP 100
    IEHNVDKELG EEKKKTIPIL AKRAACRKYA NKWIKTQKPE FKRLGVLGDW 150
    EDPYLTINYS YEAAIAREFN KFLLSGSVVR NRKPVYWCST CTTALAEAEV 200
    EYHDHTSPSI YVKFPVIEDF SDVDPALAGD KTFVVIWTTT PWTLPSNTAV 250
    AFHPKFQYAA VAVGEETWVL AEDLVEKFMQ EVGIEDYSIK STFTAEKLEN 300
    RNCRHPFMDR DSRLVFADYV TTEAGTGCVH TAPGHGADDY ATGLRYGLEV 350
    LSPVDGDGIY TKEAGPYAGR QVPEVNSDII ADLAESGLLV FKKDINHSYP 400
    HCWRCRKPVM YRATPQWFIS MENNDLRKKA LKNIESVSWT PSWGMNRIHS 450
    MVESRPDWCL SRQRTWGVPL TVISCKDCGE VVKSEEVVAK IDELFLKEGA 500
    DAWFSHPVED FLPEGHVCTC GCATFIKEED ILDVWFDSGS SFAAVCELRD 550
    DLVAPADLYL EGSDQHRGWF QSSLITATGT RGYAPFKGVL THGYVVDGQG 600
    KKMSKSVGNV VAPQEVIDEY GAEILRLWVS SEDYRDDVKV SKEILKQVSD 650
    SYRKIRNTIR YFLGNLNDFD PSKDRIAVSE MSELDRWALA RFEELRAKIT 700
    ESYDKYEFHA INQSLNYFCG TTMSAFYLDI IKDRLYVEGT DSTIRRASQT 750
    VLYDILDGLL RLMSPVLSFT AADAWNALYS LGEKDSLEKS VFFADFPVAI 800
    DPQFDAEQEA RWQRLIKIRS ELTKALELAR RDKVIGHSLE AEVLVKGEGE 850
    LGEFIHAEWQ HLREISIVSA MSEIEGAPEE SAYVSEEVEG LVVSVKLAPG 900
    VKCDRCWIRS TTVGDSVEHP QLCSRCLAIV EDMDLEMDA 939
    Length:939
    Mass (Da):106,436
    Last modified:September 13, 2004 - v1
    Checksum:iAD7AC4EC2F193A8E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR522870 Genomic DNA. Translation: CAG37281.1.
    RefSeqiWP_011189793.1. NC_006138.1.
    YP_066288.1. NC_006138.1.

    Genome annotation databases

    EnsemblBacteriaiCAG37281; CAG37281; DP2552.
    GeneIDi2945479.
    KEGGidps:DP2552.
    PATRICi21715208. VBIDesPsy67261_2780.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR522870 Genomic DNA. Translation: CAG37281.1 .
    RefSeqi WP_011189793.1. NC_006138.1.
    YP_066288.1. NC_006138.1.

    3D structure databases

    ProteinModelPortali Q6AK45.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 177439.DP2552.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG37281 ; CAG37281 ; DP2552 .
    GeneIDi 2945479.
    KEGGi dps:DP2552.
    PATRICi 21715208. VBIDesPsy67261_2780.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci DPSY177439:GJW5-2633-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LSv54 / DSM 12343.

    Entry informationi

    Entry nameiSYI_DESPS
    AccessioniPrimary (citable) accession number: Q6AK45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3