Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDPSY177439:GJW5-2633-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:DP2552
OrganismiDesulfotalea psychrophila (strain LSv54 / DSM 12343)
Taxonomic identifieri177439 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea
ProteomesiUP000000602 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligasePRO_0000098379Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi177439.DP2552.

Structurei

3D structure databases

ProteinModelPortaliQ6AK45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AK45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYRDSLNLP KTNFKMKANL AQREPMILKR WEKEGLYQML QERAQDRPLF
60 70 80 90 100
VLHDGPPYAN GHIHLGHAFN KILKDIILRS KRASGFNAPY VPGWDCHGLP
110 120 130 140 150
IEHNVDKELG EEKKKTIPIL AKRAACRKYA NKWIKTQKPE FKRLGVLGDW
160 170 180 190 200
EDPYLTINYS YEAAIAREFN KFLLSGSVVR NRKPVYWCST CTTALAEAEV
210 220 230 240 250
EYHDHTSPSI YVKFPVIEDF SDVDPALAGD KTFVVIWTTT PWTLPSNTAV
260 270 280 290 300
AFHPKFQYAA VAVGEETWVL AEDLVEKFMQ EVGIEDYSIK STFTAEKLEN
310 320 330 340 350
RNCRHPFMDR DSRLVFADYV TTEAGTGCVH TAPGHGADDY ATGLRYGLEV
360 370 380 390 400
LSPVDGDGIY TKEAGPYAGR QVPEVNSDII ADLAESGLLV FKKDINHSYP
410 420 430 440 450
HCWRCRKPVM YRATPQWFIS MENNDLRKKA LKNIESVSWT PSWGMNRIHS
460 470 480 490 500
MVESRPDWCL SRQRTWGVPL TVISCKDCGE VVKSEEVVAK IDELFLKEGA
510 520 530 540 550
DAWFSHPVED FLPEGHVCTC GCATFIKEED ILDVWFDSGS SFAAVCELRD
560 570 580 590 600
DLVAPADLYL EGSDQHRGWF QSSLITATGT RGYAPFKGVL THGYVVDGQG
610 620 630 640 650
KKMSKSVGNV VAPQEVIDEY GAEILRLWVS SEDYRDDVKV SKEILKQVSD
660 670 680 690 700
SYRKIRNTIR YFLGNLNDFD PSKDRIAVSE MSELDRWALA RFEELRAKIT
710 720 730 740 750
ESYDKYEFHA INQSLNYFCG TTMSAFYLDI IKDRLYVEGT DSTIRRASQT
760 770 780 790 800
VLYDILDGLL RLMSPVLSFT AADAWNALYS LGEKDSLEKS VFFADFPVAI
810 820 830 840 850
DPQFDAEQEA RWQRLIKIRS ELTKALELAR RDKVIGHSLE AEVLVKGEGE
860 870 880 890 900
LGEFIHAEWQ HLREISIVSA MSEIEGAPEE SAYVSEEVEG LVVSVKLAPG
910 920 930
VKCDRCWIRS TTVGDSVEHP QLCSRCLAIV EDMDLEMDA
Length:939
Mass (Da):106,436
Last modified:September 13, 2004 - v1
Checksum:iAD7AC4EC2F193A8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR522870 Genomic DNA. Translation: CAG37281.1.
RefSeqiYP_066288.1. NC_006138.1.

Genome annotation databases

EnsemblBacteriaiCAG37281; CAG37281; DP2552.
KEGGidps:DP2552.
PATRICi21715208. VBIDesPsy67261_2780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR522870 Genomic DNA. Translation: CAG37281.1.
RefSeqiYP_066288.1. NC_006138.1.

3D structure databases

ProteinModelPortaliQ6AK45.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi177439.DP2552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG37281; CAG37281; DP2552.
KEGGidps:DP2552.
PATRICi21715208. VBIDesPsy67261_2780.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciDPSY177439:GJW5-2633-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LSv54 / DSM 12343.

Entry informationi

Entry nameiSYI_DESPS
AccessioniPrimary (citable) accession number: Q6AK45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2004
Last modified: April 29, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.