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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi484 – 4841MagnesiumUniRule annotation
Metal bindingi490 – 4901MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciDPSY177439:GJW5-2689-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:DP2608
OrganismiDesulfotalea psychrophila (strain LSv54 / DSM 12343)
Taxonomic identifieri177439 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobulbaceaeDesulfotalea
ProteomesiUP000000602 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Polyribonucleotide nucleotidyltransferasePRO_0000329623Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi177439.DP2608.

Structurei

3D structure databases

ProteinModelPortaliQ6AJY9.
SMRiQ6AJY9. Positions 615-688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini551 – 61464KHUniRule annotationAdd
BLAST
Domaini620 – 68869S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AJY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNKVEADID GKLISIETGK LAKQASGSIV ISCGGTVVLV TVVGAKTSNP
60 70 80 90 100
NVDFLPLTIE YQEKFSAVGR VPGNYFRREI GRPSDQEVLT CRIIDRPLRP
110 120 130 140 150
LFPEGYRSET QVIATVLSAD QVNSPDVLAL TGASAALSIS HIPFAGPVAG
160 170 180 190 200
GRIAYIDGKY VLNPTFAELE SSSMDLMVAC TRNAIVMVEG KADELSEDEI
210 220 230 240 250
LGAIFFAFEN LQPLINLQDE LQRANGLEKI VVESPVIDAE LKAKVVEIAA
260 270 280 290 300
AGMQKVYTTV DKLERGKVYD DLKKEVVAQL DTEEGLRASE ISSLLSDFKK
310 320 330 340 350
TYLREKTVNT GVRIGGRAWD EIRDISSETE YLPKTHGSAL FTRGETQALV
360 370 380 390 400
TATLGSERDK QRVETLNGET SRRFMLHYNF PPYCVGEARF LRGPSRRDIG
410 420 430 440 450
HGTLATRGLE AVLPSEEDFP YAIRVVSEIL ESNGSSSMAS VCGGSMAMMD
460 470 480 490 500
AGVPLKRPVS GIAMGLIKEG EGVAILSDIL GDEDHLGDMD FKVVGTCDGI
510 520 530 540 550
TALQMDIKID GVSRDIMSNA LDQAKRGRMH ILEEMSKGIA EARAEVVEHA
560 570 580 590 600
PKYFIHKISQ DKIRDIIGPG GKIIKELSAQ YDAKIEVDDS GLVKMFVANG
610 620 630 640 650
ALAEALVERV KSITAEVEVG AVYTGKVKTI KDFGAFVEIL PGTDGLVHIS
660 670 680 690
ELALERVKDV TDVVNEGDTI EVKVLDVDNR GRIRLSRKAL LG
Length:692
Mass (Da):75,082
Last modified:September 12, 2004 - v1
Checksum:iF379F8487718AFED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR522870 Genomic DNA. Translation: CAG37337.1.
RefSeqiYP_066344.1. NC_006138.1.

Genome annotation databases

EnsemblBacteriaiCAG37337; CAG37337; DP2608.
KEGGidps:DP2608.
PATRICi21715326. VBIDesPsy67261_2839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR522870 Genomic DNA. Translation: CAG37337.1.
RefSeqiYP_066344.1. NC_006138.1.

3D structure databases

ProteinModelPortaliQ6AJY9.
SMRiQ6AJY9. Positions 615-688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi177439.DP2608.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG37337; CAG37337; DP2608.
KEGGidps:DP2608.
PATRICi21715326. VBIDesPsy67261_2839.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciDPSY177439:GJW5-2689-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LSv54 / DSM 12343.

Entry informationi

Entry nameiPNP_DESPS
AccessioniPrimary (citable) accession number: Q6AJY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 28, 2008
Last sequence update: September 12, 2004
Last modified: March 31, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.