Q6AJL6 (ISPE_DESPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	DP2735

Organism

Desulfotalea psychrophila [Complete proteome] [HAMAP]

Taxonomic identifier

84980 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfobacterales › Desulfobulbaceae › Desulfotalea

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235087
Regions
	Nucleotide binding	108 – 118	11	ATP Potential
Sites
	Active site	22	1	By similarity
	Active site	150	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AJL6 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 1156BE02B8A4E33A	FASTA	299	32,616
	10 20 30 40 50 60 MDRNEGELAQ VATRLHMRAP AKVNLSLQVL SRRADGYHDL ATHMQKVSLY DELELCLTKQ 70 80 90 100 110 120 SGVSLVCSDG DIPLDEKNLA VRAALAYLAR SSRVGQRGVR ISLEKNIPVG AGLGGGSSDA 130 140 150 160 170 180 GTVLRGLNQL LDNEFSEEEL IEMARPLGAD VPFFASEMSA AFATGIGDIL QPLESTKEFD 190 200 210 220 230 240 FILVNPGIFI STKEIFERFS LTLSPKRNIF ARPFRVHERA SLLDYMHNDL EEIVSDLCPA 250 260 270 280 290 IDEMKSLLEA NGASAVMMSG SGSTVFGVFQ RSVGQKKINQ VCKVLSCKYG EKVFAVQAV

References

[1]

"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments." Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P. Environ. Microbiol. 6:887-902(2004) [PubMed: 15305914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LSv54 / DSM 12343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR522870 Genomic DNA. Translation: CAG37464.1.
RefSeq	YP_066471.1. NC_006138.1.
3D structure databases
ProteinModelPortal	Q6AJL6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2946139.
GenomeReviews	Gene locus DP2735 in contig CR522870_GR.
KEGG	dps:DP2735.
NMPDR	fig|177439.1.peg.2735.
PATRIC	21715586. VBIDesPsy67261_2967.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
Enzyme and pathway databases
BioCyc	DPSY177439:DP2735-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_DESPS

Accession

Primary (citable) accession number: Q6AJL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program