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Q6AGT6 (Q6AGT6_LEIXX) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL AAT88409.1
Ordered Locus Names:Lxx04060
OrganismLeifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]
Taxonomic identifier281090 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length980 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q6AGT6 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: FC93822943E7F1B0

FASTA980106,116
        10         20         30         40         50         60 
MWAALAAIEA ALGGGATARS SNRSCVQILA RSKVPATKKL VIVESPNKMK SIAQYLGDGY 

        70         80         90        100        110        120 
EVMASVGHIR DLIEPKNLPP ELKKGSLGKF SVDVENEFEP YYVVSDQKKK TVADLKRALK 

       130        140        150        160        170        180 
DADELLLATD EDREGEAIAW HLVEVLKPKV PVKRMVFHEI TREAIEKARD NTRQIDTALV 

       190        200        210        220        230        240 
DAQETRRILD RLYGYEVSPV LWRKVGPGLS AGRVQSAATR LVVDRERERL AFVAASYWGL 

       250        260        270        280        290        300 
TAELAPEEAP SFGARLARVD GDRIATGRDF DDHGRLTSGA RTLDEVAAQE LTEAVRKPST 

       310        320        330        340        350        360 
VVSVAKVDSK PYSRRPAAPF TTSTMQQEAA RKLRFSARQT MSVAQSLYEN GYITYMRTDS 

       370        380        390        400        410        420 
ASLSQQATNA ARAQAAKLYG PETVPEKPRV YTSKSKNAQE AHEAIRPSGE TFRTPAELEK 

       430        440        450        460        470        480 
SLRGPELRLY DLIWKRTVAS QMADAKGQTA SVTVEAAIAE GQLEGTVAEF TESGTVITFR 

       490        500        510        520        530        540 
GFLGAYEEGR DEERNASDTG ESKLPPLTAG QTLSVQEVAA DGHETTPPPR YTEASLVKSL 

       550        560        570        580        590        600 
EELGIGRPST FASIISTIID RGYVTQRGQA LVPSWVAFSV VRLLEDYFGD LVQYDFTAEM 

       610        620        630        640        650        660 
EDDLDRIAGG VAARVDWLNS FYFGSDKHMG LRRVIDNLGE IDARSINSIA IEDGVTLRIG 

       670        680        690        700        710        720 
KYGPYLELTE PAATEGTSPR RVTIPPELAP DELTPAKARE LIEAPVQTDR VLGTNPANGK 

       730        740        750        760        770        780 
LVLAKDGRFG PYVTEADPEE PEADQRTGEV IEARKPAKKA AAPKQRTASL FKSMDLASVD 

       790        800        810        820        830        840 
LDTALRLLDL PRVVGVDPES GDEIQAQNGR YGPYLKKGTD TRSLPGEDDI FAVDLVGALD 

       850        860        870        880        890        900 
LFAQPKYGNR RVSSALKEFD PDPVSGKPIK VKDGRFGPYV TDGETNATIP RGETVDEVDF 

       910        920        930        940        950        960 
ERAVQLLADK RVKGPAKKTA SRASAAKKTT AKPAAAKKTA AATSAAATST AEKTTAAKKA 

       970        980 
AAAAKTTAST AAEPTAADAE

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016822 Genomic DNA. Translation: AAT88409.1.
RefSeqYP_061514.1. NC_006087.1.

3D structure databases

ProteinModelPortalQ6AGT6.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2940335.
GenomeReviewsGene locus Lxx04060 in contig AE016822_GR.
KEGGlxx:Lxx04060.
NMPDRfig|281090.3.peg.1436.
PATRIC22334290. VBILeiXyl11655_0411.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG512935.
OMAKKVMPRL.
ProtClustDBCLSK831046.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ6AGT6_LEIXX
AccessionPrimary (citable) accession number: Q6AGT6
Entry history
Integrated into UniProtKB/TrEMBL: September 13, 2004
Last sequence update: September 13, 2004
Last modified: December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info