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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Leifsonia xyli subsp. xyli (strain CTCB07)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei84 – 841SubstrateUniRule annotation
Binding sitei229 – 2291Substrate; via amide nitrogenUniRule annotation
Metal bindingi236 – 2361ManganeseUniRule annotation
Binding sitei236 – 2361SubstrateUniRule annotation
Metal bindingi256 – 2561Manganese; via tele nitrogenUniRule annotation
Binding sitei278 – 2781SubstrateUniRule annotation
Active sitei280 – 2801UniRule annotation
Metal bindingi303 – 3031ManganeseUniRule annotation
Binding sitei395 – 3951GTPUniRule annotation
Binding sitei426 – 4261GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi520 – 5234GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLXYL281090:GH0X-394-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Lxx04240
OrganismiLeifsonia xyli subsp. xyli (strain CTCB07)
Taxonomic identifieri281090 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeLeifsonia
ProteomesiUP000001306 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103606Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi281090.Lxx04240.

Structurei

3D structure databases

ProteinModelPortaliQ6AGS4.
SMRiQ6AGS4. Positions 17-610.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni393 – 3953Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AGS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPPRPTAP PGAAALTGLA ALHAWVDELA ALLQPDGIVW CDGSRGQTDR
60 70 80 90 100
LTKQLVAEGK LIRLNPEWRP NSFLSRTDPS DVARVEDRTF ICSLDEADAG
110 120 130 140 150
PTNNWREPEG MRAELREAFA GSMRGRTLYV VPFSMGPIGG PLSQLGVQLT
160 170 180 190 200
DSAYVAVSMG IMTRVASAVL DLIAAGQPWV PTVHSVGAPL VDEAGVRQED
210 220 230 240 250
VAWPCNPVKY IVQFPETREI WSYGSGYGGN AILAKKCFAL RIASVMARDE
260 270 280 290 300
GWLAEHMLIV KVTSPEGRVF HFAAAFPSSC GKTNLAMLKP SIPGWTVETV
310 320 330 340 350
CDDIAWLRPG PDGRLRAINP EAGFFGVAPG TGELTNKTAV EALWGNTIFT
360 370 380 390 400
NVALRDDGDV WWEGLTAEPP AHLIDWEGND WTPASGRPAA HPNSRFTVSA
410 420 430 440 450
AQCPVIADEW DVVDGVPIDA ILFGGRRATN VPLVAQARDW KHGVFIGATI
460 470 480 490 500
SSEQTAAAEG TVGELRRDPF AMLPFCGYNM ADYWAHWLRM GEKLGADAPA
510 520 530 540 550
IFQVNWFRKG DDGRFLWPGF SENARVIEWI ARRVEGSAGA EETAIGRLPL
560 570 580 590 600
ADELDLDGLG LPAEDLAALF DIDTERWLAE CALTEQYFAR FGPRVPPALT
610
AELASLRDKL RA
Length:612
Mass (Da):66,419
Last modified:September 13, 2004 - v1
Checksum:iBB6BD41B5049CE6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA. Translation: AAT88421.1.
RefSeqiYP_061526.1. NC_006087.1.

Genome annotation databases

EnsemblBacteriaiAAT88421; AAT88421; Lxx04240.
KEGGilxx:Lxx04240.
PATRICi22334330. VBILeiXyl11655_0430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA. Translation: AAT88421.1.
RefSeqiYP_061526.1. NC_006087.1.

3D structure databases

ProteinModelPortaliQ6AGS4.
SMRiQ6AGS4. Positions 17-610.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi281090.Lxx04240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT88421; AAT88421; Lxx04240.
KEGGilxx:Lxx04240.
PATRICi22334330. VBILeiXyl11655_0430.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciLXYL281090:GH0X-394-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CTCB07.

Entry informationi

Entry nameiPCKG_LEIXX
AccessioniPrimary (citable) accession number: Q6AGS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2005
Last sequence update: September 13, 2004
Last modified: April 1, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.