Phosphoenolpyruvate carboxykinase [GTP] - Leifsonia xyli subsp. xyli (strain CTCB07)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32

Alternative name(s):
Phosphoenolpyruvate carboxylase

Gene names

Name:	pckG
Ordered Locus Names:	Lxx04240

Organism

Leifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]

Taxonomic identifier

281090 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia ›

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452
Catalytic activity	GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO₂. HAMAP-Rule MF_00452
Cofactor	Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00452
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00452.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	GTP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxykinase (GTP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 612

612

Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452

PRO_0000103606

Regions

Nucleotide binding

520 – 523

4

GTP By similarity

Region

393 – 395

3

Substrate binding By similarity

Sites

Active site

280

1

By similarity

Metal binding

236

1

Manganese By similarity

Metal binding

256

1

Manganese By similarity

Metal binding

303

1

Manganese By similarity

Binding site

84

1

Substrate By similarity

Binding site

229

1

Substrate; via amide nitrogen By similarity

Binding site

236

1

Substrate By similarity

Binding site

278

1

Substrate By similarity

Binding site

395

1

GTP By similarity

Binding site

426

1

GTP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6AGS4 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: BB6BD41B5049CE6A
Show »

FASTA

612

66,419

        10         20         30         40         50         60 
MTAPPRPTAP PGAAALTGLA ALHAWVDELA ALLQPDGIVW CDGSRGQTDR LTKQLVAEGK 

        70         80         90        100        110        120 
LIRLNPEWRP NSFLSRTDPS DVARVEDRTF ICSLDEADAG PTNNWREPEG MRAELREAFA 

       130        140        150        160        170        180 
GSMRGRTLYV VPFSMGPIGG PLSQLGVQLT DSAYVAVSMG IMTRVASAVL DLIAAGQPWV 

       190        200        210        220        230        240 
PTVHSVGAPL VDEAGVRQED VAWPCNPVKY IVQFPETREI WSYGSGYGGN AILAKKCFAL 

       250        260        270        280        290        300 
RIASVMARDE GWLAEHMLIV KVTSPEGRVF HFAAAFPSSC GKTNLAMLKP SIPGWTVETV 

       310        320        330        340        350        360 
CDDIAWLRPG PDGRLRAINP EAGFFGVAPG TGELTNKTAV EALWGNTIFT NVALRDDGDV 

       370        380        390        400        410        420 
WWEGLTAEPP AHLIDWEGND WTPASGRPAA HPNSRFTVSA AQCPVIADEW DVVDGVPIDA 

       430        440        450        460        470        480 
ILFGGRRATN VPLVAQARDW KHGVFIGATI SSEQTAAAEG TVGELRRDPF AMLPFCGYNM 

       490        500        510        520        530        540 
ADYWAHWLRM GEKLGADAPA IFQVNWFRKG DDGRFLWPGF SENARVIEWI ARRVEGSAGA 

       550        560        570        580        590        600 
EETAIGRLPL ADELDLDGLG LPAEDLAALF DIDTERWLAE CALTEQYFAR FGPRVPPALT 

       610 
AELASLRDKL RA

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References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli."
Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H.

Setubal J.C.
Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CTCB07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016822 Genomic DNA. Translation: AAT88421.1.
RefSeq	YP_061526.1. NC_006087.1.
3D structure databases
ProteinModelPortal	Q6AGS4.
SMR	Q6AGS4. Positions 17-610.
ModBase	Search...
Protein-protein interaction databases
STRING	281090.Lxx04240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT88421; AAT88421; Lxx04240.
GeneID	2939161.
KEGG	lxx:Lxx04240.
PATRIC	22334330. VBILeiXyl11655_0430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1274.
HOGENOM	HOG000191700.
KO	K01596.
OMA	WMRFGED.
ProtClustDB	PRK04210.
Enzyme and pathway databases
BioCyc	LXYL281090:GH0X-394-MONOMER.
UniPathway	UPA00138.
Family and domain databases
Gene3D	3.40.449.10. 1 hit. 3.90.228.20. 2 hits.
HAMAP	MF_00452. PEPCK_GTP.
InterPro	IPR018091. PEP_carboxykin_GTP_CS. IPR013035. PEP_carboxykinase_C. IPR008209. PEP_carboxykinase_GTP. IPR008210. PEP_carboxykinase_N. [Graphical view]
PANTHER	PTHR11561. PTHR11561. 1 hit.
Pfam	PF00821. PEPCK. 1 hit. [Graphical view]
PIRSF	PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
PROSITE	PS00505. PEPCK_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKG_LEIXX

Accession

Primary (citable) accession number: Q6AGS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	September 13, 2004
Last modified:	May 29, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents