Q6AGR0 (GLPK_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Lxx04420

Organism

Leifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]

Taxonomic identifier

281090 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Glycerol kinase HAMAP MF_00186		PRO_0000059462
Regions
	Nucleotide binding	413 – 417	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	268	1	ATP By similarity
	Binding site	312	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AGR0 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: BB5175DBB1FDEF48	FASTA	506	55,326
	10 20 30 40 50 60 MAEYIVAIDQ GTTSTRAIIF DKSGSIVSTG QLEHEQIFPK PGWVEHNPVE IWNNTREVIG 70 80 90 100 110 120 QALSKADLTR HDIAAVGITN QRETAVVWDK NTGEPVYNAI VWQDTRTQPI VDRLAAEGGV 130 140 150 160 170 180 ERFKPIVGLP LATYFSGTKI VWILENVEGV RERAERGDLL FGTTESWVLW NLTGGTDGGV 190 200 210 220 230 240 HATDVTNASR TLFMDLETLS WRDDILDVFG VPKSMLPEIK SSSEVYGAVE SSSLLREVPV 250 260 270 280 290 300 AGILGDQQAA TFGQAAFDPG ESKNTYGTGN FLIFNTGEEI VHSKNGLLTT LGYKLGDGKP 310 320 330 340 350 360 HYALEGSIAV TGSLVQWLRD NLGVISSAPE IEELAQTVED NGGAYFVPAL SGLFAPYWRA 370 380 390 400 410 420 DARGALVGLT RYVNKGHIAR AALEATAFQT REVLDAVNAD SGVDLTELKV DGGMIANNTL 430 440 450 460 470 480 MQFQADILGV PVVRPVVAET TALGAAYAAG LATGFWENLD DLRKNWKEDR RWEPKMDAAE 490 500 RDRQLRLWKK AVTKTFDWVD DDVRNG

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli." Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H. , Coutinho L.L., El-Dorry H.A., Ferro M.I.T., Gagliardi P.R., Giglioti E., Goldman M.H.S., Goldman G.H., Kimura E.T., Ferro E.S., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Mauro S.M.Z., Machado M.A., Marino C.L., Menck C.F., Nunes L.R., Oliveira R.C., Pereira G.G., Siqueira W., de Souza A.A., Tsai S.M., Zanca A.S., Simpson A.J.G., Brumbley S.M., Setubal J.C. Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CTCB07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016822 Genomic DNA. Translation: AAT88435.1.
RefSeq	YP_061540.1. NC_006087.1.
3D structure databases
ProteinModelPortal	Q6AGR0.
SMR	Q6AGR0. Positions 2-503.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2939235.
GenomeReviews	Gene locus Lxx04420 in contig AE016822_GR.
KEGG	lxx:Lxx04420.
NMPDR	fig|281090.3.peg.1462.
PATRIC	22334368. VBILeiXyl11655_0449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	ATYFSGT.
ProtClustDB	CLSK928226.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX04420-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_LEIXX

Accession

Primary (citable) accession number: Q6AGR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program