Reviewed, UniProtKB/Swiss-Prot Q6AFZ8 (CLPP1_LEIXX)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease proteolytic subunit 1
    EC=3.4.21.92
Alternative name(s):
    Endopeptidase Clp 1

Gene names

Name:	clpP1
Ordered Locus Names:	Lxx07850

Organism

Leifsonia xyli subsp. xyli [Complete proteome] [HAMAP]

Taxonomic identifier

59736 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity.
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	ATP-dependent Clp protease proteolytic subunit 1 HAMAP MF_00444		PRO_0000179579
Sites
	Active site	88	1	By similarity
	Active site	113	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AFZ8-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 23FCC2FA4A36EE5F	FASTA	197	21,159
	10 20 30 40 50 60 MADMGIQPSV FDRLLKDRII WLGSEVRDEN ANEIAAKLLL LAAEDSKRDI YLYINSPGGS 70 80 90 100 110 120 ITAGMAIYDT MQFVPNDIVT VGIGMAASMG QLLLTAGTKG KRYITPNARV LLHQPHGGFG 130 140 150 160 170 180 GTASDIRTQA QLILHMKKRL AEITSEATGK TVDQVNADGD RDRWFTAQEA LDYGFVDHIR 190 ESALLVAGGG GTAPAGK

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli." Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H. , Coutinho L.L., El-Dorry H.A., Ferro M.I.T., Gagliardi P.R., Giglioti E., Goldman M.H.S., Goldman G.H., Kimura E.T., Ferro E.S., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Mauro S.M.Z., Machado M.A., Marino C.L., Menck C.F., Nunes L.R., Oliveira R.C., Pereira G.G., Siqueira W., de Souza A.A., Tsai S.M., Zanca A.S., Simpson A.J.G., Brumbley S.M., Setubal J.C. Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CTCB07.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016822 Genomic DNA. Translation: AAT88697.1.
RefSeq	YP_061802.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S14.008.
Genome annotation databases
GeneID	2939025.
GenomeReviews	Gene locus Lxx07850 in contig AE016822_GR.
KEGG	lxx:Lxx07850.
NMPDR	fig|281090.3.peg.172.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6AFZ8.
OMA	ANKLCAQ.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX07850-MON.
BRENDA	3.4.21.92. 297084.
Family and domain databases
HAMAP	MF_00444. [Tree]
InterPro	IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP1_LEIXX

Accession

Primary (citable) accession number: Q6AFZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	September 13, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)