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Q6AFZ0

- SYI_LEIXX

UniProt

Q6AFZ0 - SYI_LEIXX

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Leifsonia xyli subsp. xyli (strain CTCB07)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei679 – 6791ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLXYL281090:GH0X-741-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Lxx07970
    OrganismiLeifsonia xyli subsp. xyli (strain CTCB07)
    Taxonomic identifieri281090 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeLeifsonia
    ProteomesiUP000001306: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11191119Isoleucine--tRNA ligasePRO_0000098546Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi281090.Lxx07970.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6AFZ0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi84 – 9411"HIGH" regionAdd
    BLAST
    Motifi676 – 6805"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6AFZ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVPRRSRQRP ASSCRTAKTA RREMPYPLPA PDGQEPEAQP VTPSPVFPEI     50
    EEDVLAFWDR DGTFHASIDN RDGAPEWVFY DGPPFANGLP HYGHLMTGYA 100
    KDVFPRYQTM RGKKVERRFG WDTHGLPAEL EAERQLGITD KSQIEEMGLA 150
    AFNQAAKDSV LKYTREWQQY VTRQARWVDF DNGYKTLDTT YMESVIWAFK 200
    QLHEKGLAYE GYRVLPYCWR DQTPLSNHEL RMDDDVYKMR QDQTVTVTFP 250
    LTGAKAESLG LTGVRVLAWT TTPWTLPTNL ALVVGPDIQY AVVPAGPNGA 300
    ADAHGRPDDE VLSGEYLLAI DLVGNYAKDL GYGSPEAARA AVTRTIPGRE 350
    LEGVTYDRLW DYYADAATWG TQNAWQILVD GYVTTEDGTG IVHQAPAYGE 400
    DDQRVCEAAG IPVIISVDDG ARFLPAVQDV AGLQVFEAGK PLIKLLREEG 450
    RLLRQASYEH SYPHCWRCRN PLIYKAVSSW FVRVTDFRDR MVALNQEITW 500
    VPENVKDGQF GKWLAGARDW SISRNRYFGS PIPVWKSDDP DYPRVDVYGS 550
    LAELERDFGR LPLNAAGEPD LHRPFVDDLT RPNPDDPTGR STMRRIPDVL 600
    DVWFDSGSMP FAQVHYPFEN SDWFDSHNPA DFIVEYIGQA RGWFYLLHAL 650
    STALFDRPAF TNVISHGIVL GNDGQKVSKS LRNYPDVNDV FDRDGSDAMR 700
    WFLMSSPVLR GGNLVVTEEG VREGVRQVLL PLWNTWYFFS LYANATGYEA 750
    KRSTASAAVL DRYLLAKTRD LVEAVTADLD ALDSTLASAK LRDFADLLTN 800
    WYVRRSRDRF WAGVDGEGAG AEAFDTLYTV LETLTRVAAP LLPLVTERIW 850
    KDLTGGRSVH LTDWPDAAEL PADDALVAAM DRVRAISSTA LSLRKQAGLR 900
    VRLPLASLTV VAEGAAALAP FEAILRDELN VKAVCLVEGE HSGVDRALTV 950
    KARQLGPRIG KRVQEVIRAA KSGDWSEADG VVTAGGVELE PGEYELAVVI 1000
    SAPTARFENG VYIELDTALT PELEAEGLAR DIIRAVQDTR KAAGLGVSDR 1050
    IALTLAFENG GDARVLGLAT EVDIASETLA RTVQVAAAAG AFVKTFGAGQ 1100
    FANVGDFTVG ITKIEEADE 1119
    Length:1,119
    Mass (Da):124,049
    Last modified:September 13, 2004 - v1
    Checksum:i7576194652881F39
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016822 Genomic DNA. Translation: AAT88705.1.
    RefSeqiWP_011185703.1. NC_006087.1.
    YP_061810.1. NC_006087.1.

    Genome annotation databases

    EnsemblBacteriaiAAT88705; AAT88705; Lxx07970.
    GeneIDi2938988.
    KEGGilxx:Lxx07970.
    PATRICi22335128. VBILeiXyl11655_0824.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016822 Genomic DNA. Translation: AAT88705.1 .
    RefSeqi WP_011185703.1. NC_006087.1.
    YP_061810.1. NC_006087.1.

    3D structure databases

    ProteinModelPortali Q6AFZ0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 281090.Lxx07970.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT88705 ; AAT88705 ; Lxx07970 .
    GeneIDi 2938988.
    KEGGi lxx:Lxx07970.
    PATRICi 22335128. VBILeiXyl11655_0824.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LXYL281090:GH0X-741-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CTCB07.

    Entry informationi

    Entry nameiSYI_LEIXX
    AccessioniPrimary (citable) accession number: Q6AFZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3