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Q6AFZ0

- SYI_LEIXX

UniProt

Q6AFZ0 - SYI_LEIXX

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Leifsonia xyli subsp. xyli (strain CTCB07)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei679 – 6791ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLXYL281090:GH0X-741-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Lxx07970
OrganismiLeifsonia xyli subsp. xyli (strain CTCB07)
Taxonomic identifieri281090 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeLeifsonia
ProteomesiUP000001306: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11191119Isoleucine--tRNA ligasePRO_0000098546Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi281090.Lxx07970.

Structurei

3D structure databases

ProteinModelPortaliQ6AFZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi84 – 9411"HIGH" regionAdd
BLAST
Motifi676 – 6805"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AFZ0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVPRRSRQRP ASSCRTAKTA RREMPYPLPA PDGQEPEAQP VTPSPVFPEI
60 70 80 90 100
EEDVLAFWDR DGTFHASIDN RDGAPEWVFY DGPPFANGLP HYGHLMTGYA
110 120 130 140 150
KDVFPRYQTM RGKKVERRFG WDTHGLPAEL EAERQLGITD KSQIEEMGLA
160 170 180 190 200
AFNQAAKDSV LKYTREWQQY VTRQARWVDF DNGYKTLDTT YMESVIWAFK
210 220 230 240 250
QLHEKGLAYE GYRVLPYCWR DQTPLSNHEL RMDDDVYKMR QDQTVTVTFP
260 270 280 290 300
LTGAKAESLG LTGVRVLAWT TTPWTLPTNL ALVVGPDIQY AVVPAGPNGA
310 320 330 340 350
ADAHGRPDDE VLSGEYLLAI DLVGNYAKDL GYGSPEAARA AVTRTIPGRE
360 370 380 390 400
LEGVTYDRLW DYYADAATWG TQNAWQILVD GYVTTEDGTG IVHQAPAYGE
410 420 430 440 450
DDQRVCEAAG IPVIISVDDG ARFLPAVQDV AGLQVFEAGK PLIKLLREEG
460 470 480 490 500
RLLRQASYEH SYPHCWRCRN PLIYKAVSSW FVRVTDFRDR MVALNQEITW
510 520 530 540 550
VPENVKDGQF GKWLAGARDW SISRNRYFGS PIPVWKSDDP DYPRVDVYGS
560 570 580 590 600
LAELERDFGR LPLNAAGEPD LHRPFVDDLT RPNPDDPTGR STMRRIPDVL
610 620 630 640 650
DVWFDSGSMP FAQVHYPFEN SDWFDSHNPA DFIVEYIGQA RGWFYLLHAL
660 670 680 690 700
STALFDRPAF TNVISHGIVL GNDGQKVSKS LRNYPDVNDV FDRDGSDAMR
710 720 730 740 750
WFLMSSPVLR GGNLVVTEEG VREGVRQVLL PLWNTWYFFS LYANATGYEA
760 770 780 790 800
KRSTASAAVL DRYLLAKTRD LVEAVTADLD ALDSTLASAK LRDFADLLTN
810 820 830 840 850
WYVRRSRDRF WAGVDGEGAG AEAFDTLYTV LETLTRVAAP LLPLVTERIW
860 870 880 890 900
KDLTGGRSVH LTDWPDAAEL PADDALVAAM DRVRAISSTA LSLRKQAGLR
910 920 930 940 950
VRLPLASLTV VAEGAAALAP FEAILRDELN VKAVCLVEGE HSGVDRALTV
960 970 980 990 1000
KARQLGPRIG KRVQEVIRAA KSGDWSEADG VVTAGGVELE PGEYELAVVI
1010 1020 1030 1040 1050
SAPTARFENG VYIELDTALT PELEAEGLAR DIIRAVQDTR KAAGLGVSDR
1060 1070 1080 1090 1100
IALTLAFENG GDARVLGLAT EVDIASETLA RTVQVAAAAG AFVKTFGAGQ
1110
FANVGDFTVG ITKIEEADE
Length:1,119
Mass (Da):124,049
Last modified:September 13, 2004 - v1
Checksum:i7576194652881F39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA. Translation: AAT88705.1.
RefSeqiWP_011185703.1. NC_006087.1.
YP_061810.1. NC_006087.1.

Genome annotation databases

EnsemblBacteriaiAAT88705; AAT88705; Lxx07970.
GeneIDi2938988.
KEGGilxx:Lxx07970.
PATRICi22335128. VBILeiXyl11655_0824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA. Translation: AAT88705.1 .
RefSeqi WP_011185703.1. NC_006087.1.
YP_061810.1. NC_006087.1.

3D structure databases

ProteinModelPortali Q6AFZ0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 281090.Lxx07970.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT88705 ; AAT88705 ; Lxx07970 .
GeneIDi 2938988.
KEGGi lxx:Lxx07970.
PATRICi 22335128. VBILeiXyl11655_0824.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LXYL281090:GH0X-741-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CTCB07.

Entry informationi

Entry nameiSYI_LEIXX
AccessioniPrimary (citable) accession number: Q6AFZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2004
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3