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Q6AFZ0

- SYI_LEIXX

UniProt

Q6AFZ0 - SYI_LEIXX

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Lxx07970
Organism
Leifsonia xyli subsp. xyli (strain CTCB07)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei679 – 6791ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLXYL281090:GH0X-741-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Lxx07970
OrganismiLeifsonia xyli subsp. xyli (strain CTCB07)
Taxonomic identifieri281090 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeLeifsonia
ProteomesiUP000001306: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11191119Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098546Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi281090.Lxx07970.

Structurei

3D structure databases

ProteinModelPortaliQ6AFZ0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi84 – 9411"HIGH" regionUniRule annotation
Add
BLAST
Motifi676 – 6805"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AFZ0-1 [UniParc]FASTAAdd to Basket

« Hide

MVPRRSRQRP ASSCRTAKTA RREMPYPLPA PDGQEPEAQP VTPSPVFPEI     50
EEDVLAFWDR DGTFHASIDN RDGAPEWVFY DGPPFANGLP HYGHLMTGYA 100
KDVFPRYQTM RGKKVERRFG WDTHGLPAEL EAERQLGITD KSQIEEMGLA 150
AFNQAAKDSV LKYTREWQQY VTRQARWVDF DNGYKTLDTT YMESVIWAFK 200
QLHEKGLAYE GYRVLPYCWR DQTPLSNHEL RMDDDVYKMR QDQTVTVTFP 250
LTGAKAESLG LTGVRVLAWT TTPWTLPTNL ALVVGPDIQY AVVPAGPNGA 300
ADAHGRPDDE VLSGEYLLAI DLVGNYAKDL GYGSPEAARA AVTRTIPGRE 350
LEGVTYDRLW DYYADAATWG TQNAWQILVD GYVTTEDGTG IVHQAPAYGE 400
DDQRVCEAAG IPVIISVDDG ARFLPAVQDV AGLQVFEAGK PLIKLLREEG 450
RLLRQASYEH SYPHCWRCRN PLIYKAVSSW FVRVTDFRDR MVALNQEITW 500
VPENVKDGQF GKWLAGARDW SISRNRYFGS PIPVWKSDDP DYPRVDVYGS 550
LAELERDFGR LPLNAAGEPD LHRPFVDDLT RPNPDDPTGR STMRRIPDVL 600
DVWFDSGSMP FAQVHYPFEN SDWFDSHNPA DFIVEYIGQA RGWFYLLHAL 650
STALFDRPAF TNVISHGIVL GNDGQKVSKS LRNYPDVNDV FDRDGSDAMR 700
WFLMSSPVLR GGNLVVTEEG VREGVRQVLL PLWNTWYFFS LYANATGYEA 750
KRSTASAAVL DRYLLAKTRD LVEAVTADLD ALDSTLASAK LRDFADLLTN 800
WYVRRSRDRF WAGVDGEGAG AEAFDTLYTV LETLTRVAAP LLPLVTERIW 850
KDLTGGRSVH LTDWPDAAEL PADDALVAAM DRVRAISSTA LSLRKQAGLR 900
VRLPLASLTV VAEGAAALAP FEAILRDELN VKAVCLVEGE HSGVDRALTV 950
KARQLGPRIG KRVQEVIRAA KSGDWSEADG VVTAGGVELE PGEYELAVVI 1000
SAPTARFENG VYIELDTALT PELEAEGLAR DIIRAVQDTR KAAGLGVSDR 1050
IALTLAFENG GDARVLGLAT EVDIASETLA RTVQVAAAAG AFVKTFGAGQ 1100
FANVGDFTVG ITKIEEADE 1119
Length:1,119
Mass (Da):124,049
Last modified:September 13, 2004 - v1
Checksum:i7576194652881F39
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016822 Genomic DNA. Translation: AAT88705.1.
RefSeqiWP_011185703.1. NC_006087.1.
YP_061810.1. NC_006087.1.

Genome annotation databases

EnsemblBacteriaiAAT88705; AAT88705; Lxx07970.
GeneIDi2938988.
KEGGilxx:Lxx07970.
PATRICi22335128. VBILeiXyl11655_0824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016822 Genomic DNA. Translation: AAT88705.1 .
RefSeqi WP_011185703.1. NC_006087.1.
YP_061810.1. NC_006087.1.

3D structure databases

ProteinModelPortali Q6AFZ0.
ModBasei Search...

Protein-protein interaction databases

STRINGi 281090.Lxx07970.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT88705 ; AAT88705 ; Lxx07970 .
GeneIDi 2938988.
KEGGi lxx:Lxx07970.
PATRICi 22335128. VBILeiXyl11655_0824.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LXYL281090:GH0X-741-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CTCB07.

Entry informationi

Entry nameiSYI_LEIXX
AccessioniPrimary (citable) accession number: Q6AFZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2004
Last modified: September 3, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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