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Q6AFR0 (TRMB_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Lxx08920
OrganismLeifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]
Taxonomic identifier281090 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeLeifsonia

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171340

Regions

Region205 – 2084Substrate binding By similarity

Sites

Active site1321 By similarity
Binding site571S-adenosyl-L-methionine By similarity
Binding site821S-adenosyl-L-methionine By similarity
Binding site1091S-adenosyl-L-methionine By similarity
Binding site1321S-adenosyl-L-methionine By similarity
Binding site1361Substrate By similarity
Binding site1681Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6AFR0 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 1F661B4CDB39E414

FASTA22725,120
        10         20         30         40         50         60 
MEPAPLVRPR SYVVRGRKTD AQQRAIAEFW PAFGVDFAGG LLDLDALFGR TAPRTVEIGF 

        70         80         90        100        110        120 
GNGENLLALA ERHPERDFLG VEVHGAGVGR VFAAMRERGL SNIRVIRHDA VEVFETGLSA 

       130        140        150        160        170        180 
GSVAEALLFF PDPWPKARHH RRRLVQPDVV RLLARALAAD GVLRLATDWE PYADHMLAVL 

       190        200        210        220 
DAEPVLVNVA GPGAFIPRPE ARPVTKFERR GEKLGHSIFD LEYRPLP 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016822 Genomic DNA. Translation: AAT88785.1.
RefSeqYP_061890.1. NC_006087.1.

3D structure databases

ProteinModelPortalQ6AFR0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING281090.Lxx08920.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT88785; AAT88785; Lxx08920.
GeneID2940179.
KEGGlxx:Lxx08920.
PATRIC22335338. VBILeiXyl11655_0925.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMALHIATDH.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycLXYL281090:GH0X-833-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_LEIXX
AccessionPrimary (citable) accession number: Q6AFR0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: September 13, 2004
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways