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Q6AFI0 (TYSY_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:Lxx09930
OrganismLeifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]
Taxonomic identifier281090 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeLeifsonia

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140974

Sites

Active site1511 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6AFI0 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 3B3EE3B172FA00D0

FASTA26930,672
        10         20         30         40         50         60 
MTTPIPTPYE DLLRDVLANG SHKSDRTGTG TRSVFGRQLR FDLADGFPLI TTKRVHFKSI 

        70         80         90        100        110        120 
AYELLWFLRG DSNVGWLREN GVSIWDEWAD ERGELGPVYG VQWRSWPSPD GTHIDQIQQV 

       130        140        150        160        170        180 
VDTLRRDPDS RRIIVSAWNV ADIPDMALAP CHAFFQFYVA DGKLSCQLYQ RSADMFLGVP 

       190        200        210        220        230        240 
FNIASYALLT LMVAQQVGLE PGEFVWTGGD VHIYDNHIEQ VNEQLSRNPF PAPALRFARR 

       250        260 
PESVFDYRYE DFVLEGYRHH PAIRAAVAV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016822 Genomic DNA. Translation: AAT88865.1.
RefSeqYP_061970.1. NC_006087.1.

3D structure databases

ProteinModelPortalQ6AFI0.
SMRQ6AFI0. Positions 9-269.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING281090.Lxx09930.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT88865; AAT88865; Lxx09930.
GeneID2939820.
KEGGlxx:Lxx09930.
PATRIC22335550. VBILeiXyl11655_1029.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMAFGRQIRY.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycLXYL281090:GH0X-930-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_LEIXX
AccessionPrimary (citable) accession number: Q6AFI0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: September 13, 2004
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways