Q6AFI0 (TYSY_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Lxx09930

Organism

Leifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]

Taxonomic identifier

281090 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Thymidylate synthase HAMAP MF_00008		PRO_0000140974
Sites
	Active site	151	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AFI0 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 3B3EE3B172FA00D0	FASTA	269	30,672
	10 20 30 40 50 60 MTTPIPTPYE DLLRDVLANG SHKSDRTGTG TRSVFGRQLR FDLADGFPLI TTKRVHFKSI 70 80 90 100 110 120 AYELLWFLRG DSNVGWLREN GVSIWDEWAD ERGELGPVYG VQWRSWPSPD GTHIDQIQQV 130 140 150 160 170 180 VDTLRRDPDS RRIIVSAWNV ADIPDMALAP CHAFFQFYVA DGKLSCQLYQ RSADMFLGVP 190 200 210 220 230 240 FNIASYALLT LMVAQQVGLE PGEFVWTGGD VHIYDNHIEQ VNEQLSRNPF PAPALRFARR 250 260 PESVFDYRYE DFVLEGYRHH PAIRAAVAV

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli." Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H. , Coutinho L.L., El-Dorry H.A., Ferro M.I.T., Gagliardi P.R., Giglioti E., Goldman M.H.S., Goldman G.H., Kimura E.T., Ferro E.S., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Mauro S.M.Z., Machado M.A., Marino C.L., Menck C.F., Nunes L.R., Oliveira R.C., Pereira G.G., Siqueira W., de Souza A.A., Tsai S.M., Zanca A.S., Simpson A.J.G., Brumbley S.M., Setubal J.C. Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CTCB07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016822 Genomic DNA. Translation: AAT88865.1.
RefSeq	YP_061970.1. NC_006087.1.
3D structure databases
ProteinModelPortal	Q6AFI0.
SMR	Q6AFI0. Positions 9-269.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2939820.
GenomeReviews	Gene locus Lxx09930 in contig AE016822_GR.
KEGG	lxx:Lxx09930.
NMPDR	fig|281090.3.peg.356.
PATRIC	22335550. VBILeiXyl11655_1029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG588098.
OMA	RMALAPC.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX09930-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_LEIXX

Accession

Primary (citable) accession number: Q6AFI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program