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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Leifsonia xyli subsp. xyli (strain CTCB07)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:Lxx10020
OrganismiLeifsonia xyli subsp. xyli (strain CTCB07)
Taxonomic identifieri281090 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeLeifsonia
Proteomesi
  • UP000001306 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092511 – 1007Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST1007

Interactioni

Protein-protein interaction databases

STRINGi281090.Lxx10020.

Structurei

3D structure databases

ProteinModelPortaliQ6AFH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 496Adenylyl removaseUniRule annotationAdd BLAST496
Regioni505 – 1007Adenylyl transferaseUniRule annotationAdd BLAST503

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000243040.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG093H00LJ.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q6AFH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTREQLSLTV LARAGFVGLS SVRAELEELA TLTGFPVDDL LPALLAAADP
60 70 80 90 100
DTALRLALRL LRRAPDQAAW FLRSWKDARR LLRVIGASEG AAEFFLRQPV
110 120 130 140 150
ELASLHRPVT ALPTAAELRE DLLDAVGAVG GFASVAEEEA WTALRVRYRR
160 170 180 190 200
RLVQLASFDL EQDDPVAGFD GVAAALSDLA GAALDASLAV ARRQASGSGP
210 220 230 240 250
GRFPEAEVRA TRFAIIGMGK AGARELNYVS DVDVIYVTDG AEDAGVPPGR
260 270 280 290 300
AVDIATRLAV LTQRGIQDPA LEPGLWEVDS NLRPEGRDGA LVRTLDSHLA
310 320 330 340 350
YYDRWAKGWE FQALLKARSL AGDWELGERY VTALAPRVWS SASRENFVES
360 370 380 390 400
VQRMRERVTD NIPDGDLHYQ LKLGPGGLRD VEFTVQLLQL VHGQTDGLVR
410 420 430 440 450
QRDTLSALAA LAGQSYIGRE EAAAFSHDYR TLRLLEHRLQ LRHLRRTHLM
460 470 480 490 500
PRDEVEVRIL ARATGLAASA GQLLTQWNEI KHRVRGLHER LFYRPLLSAV
510 520 530 540 550
AAMPNEDARL SGEAGLTSEQ AQARLAAIGF RDPRGALAHI AALTAGVSRR
560 570 580 590 600
ATIQRHLLPV MLQWFSAGAD PDYGLLSFRR LSDDLGGTHW YLRMLRDSSG
610 620 630 640 650
AAERLTRVLS GSRFVAELLG RIPESVAWLE SEEELRPRAP ELLREETAAI
660 670 680 690 700
LARHESAETA AAALRAVRRR EVLRLAFSGI LGFSTIEELA RGLSAVTENL
710 720 730 740 750
LTGVLGAIRA ARDDAAALEF AIIGMGRFGG RELGFGSDAD IMYVFRPLAA
760 770 780 790 800
GQDAAHRAAT AIVADLNRLT EDSALPVDLD IGLRPEGKNG PSVRSLDSYR
810 820 830 840 850
AYYARWSLAW EAQALLRARG VAGDSALIGD FETLADEVRY PASIGEQAVR
860 870 880 890 900
EVKRIKARIE NERLPQGADP ARHLKLGRGS LSDVEWFVQL VQLQHAAAHP
910 920 930 940 950
ALRTPSTLDA LAVAAGEGFV SGEDAARLRA AWVFASRARS AMTLWTNKTA
960 970 980 990 1000
DVLPFDRVVL DGVARLLEYP PGAASRMEED YLAVTRRARA VFEREFYGPP

QRPATTA
Length:1,007
Mass (Da):110,040
Last modified:September 13, 2004 - v1
Checksum:i9C23C90309BAA2DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA. Translation: AAT88873.1.
RefSeqiWP_011185870.1. NC_006087.1.

Genome annotation databases

EnsemblBacteriaiAAT88873; AAT88873; Lxx10020.
KEGGilxx:Lxx10020.
PATRICi22335568. VBILeiXyl11655_1038.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA. Translation: AAT88873.1.
RefSeqiWP_011185870.1. NC_006087.1.

3D structure databases

ProteinModelPortaliQ6AFH2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi281090.Lxx10020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT88873; AAT88873; Lxx10020.
KEGGilxx:Lxx10020.
PATRICi22335568. VBILeiXyl11655_1038.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000243040.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG093H00LJ.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_LEIXX
AccessioniPrimary (citable) accession number: Q6AFH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: September 13, 2004
Last modified: February 15, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.