Q6AFA1 (SYA_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Lxx10910

Organism

Leifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]

Taxonomic identifier

281090 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000075135
Sites
	Metal binding	572	1	Zinc Potential
	Metal binding	576	1	Zinc Potential
	Metal binding	675	1	Zinc Potential
	Metal binding	679	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6AFA1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 9FC15F35FE43F6EA	FASTA	885	94,580
	10 20 30 40 50 60 MQTAEIHRRW LDFFARRGHT VVPSASLVSD DPSLLFTVAG MVPFVPYLTG IVPAPFPRAT 70 80 90 100 110 120 SVQKCIRTLD IEEVGKTPRH GTFFQMCGNF SFGDYFKEQA IAFAWDLLTT AETEGGLGFD 130 140 150 160 170 180 PKDLWVTVYE EDDEAREIWR RVSGLAEERI QGLGKDTNYW STGQPGPAGP CSEIFFDRGP 190 200 210 220 230 240 AYGIDGGPAT DDDRYVEIWN LVFMQFLRGE GTGKDDFEIL GDLPKKNIDT GLGLERVAFL 250 260 270 280 290 300 KQGVENMYEI DQVRPVLDRA AELAGKPYGN EAHEDDVRLR VVADHVRSAL MLMTDGVTPS 310 320 330 340 350 360 NEGRGYVLRR LLRRTVRAMW LLGVEAATFP ALFPVSRDAM KAAYPEVETE FARTSQLAYA 370 380 390 400 410 420 EEETFLRTLV AGTSILDTAV ANTQKAGKRE LAGDTAFLLH DTYGFPIDLT LEMAEEAGLS 430 440 450 460 470 480 VDRAAFDTLM ADQRARAKAD AKAKKTALAD LSVYSGLRAL GETVFTGYTE LETESSVLGL 490 500 510 520 530 540 IIDGHSANKA VEGQVAEVIL GATALYAEAG GQDADTGTIV GPGYVLDVLD VQKPVRGLVS 550 560 570 580 590 600 HRVLVRSGEV GVGVPATSLV DADYRRGAKQ AHSGTHIIHA ALRQVLGSNA HQSGSYNKAG 610 620 630 640 650 660 YLRLDFSWNQ ALSPETRSEI EEISNSAIRQ NLEVTTRELP LAEAKALGAM ALFGEKYGDT 670 680 690 700 710 720 VRVVDIGGPW SRELCAGTHV ARSAEIGLIN LVSESSVGST NRRVESLVGL EAFKDLAVER 730 740 750 760 770 780 TIVSQLSSSL KTPREQLPEK IADLMASLKA AEKRIQAFEA RAVLDRVQGL LEAVSRRGAV 790 800 810 820 830 840 QVVAADAGTL STADDLRLLA ITVRDRLGSD PATVALAALA GGKPVVIVAT NQAARDAGVT 850 860 870 880 AGALAKTAAG VLGGGGGGKA DLAQGGGTDA TAIPAALAAV STAIG

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli." Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H. , Coutinho L.L., El-Dorry H.A., Ferro M.I.T., Gagliardi P.R., Giglioti E., Goldman M.H.S., Goldman G.H., Kimura E.T., Ferro E.S., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Mauro S.M.Z., Machado M.A., Marino C.L., Menck C.F., Nunes L.R., Oliveira R.C., Pereira G.G., Siqueira W., de Souza A.A., Tsai S.M., Zanca A.S., Simpson A.J.G., Brumbley S.M., Setubal J.C. Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CTCB07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016822 Genomic DNA. Translation: AAT88944.1.
RefSeq	YP_062049.1. NC_006087.1.
3D structure databases
ProteinModelPortal	Q6AFA1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2938703.
GenomeReviews	Gene locus Lxx10910 in contig AE016822_GR.
KEGG	lxx:Lxx10910.
NMPDR	fig|281090.3.peg.463.
PATRIC	22335754. VBILeiXyl11655_1127.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	GESKTDQ.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX10910-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_LEIXX

Accession

Primary (citable) accession number: Q6AFA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program