Q6AF87 (PYRC_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Lxx11070

Organism

Leifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]

Taxonomic identifier

281090 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220_B
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00220_B
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220_B
Subunit structure	Homodimer By similarity. HAMAP MF_00220_B
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Dihydroorotase HAMAP MF_00220_B		PRO_0000147238
Sites
	Metal binding	58	1	Zinc 1 By similarity
	Metal binding	60	1	Zinc 1 By similarity
	Metal binding	179	1	Zinc 2 By similarity
	Metal binding	232	1	Zinc 2 By similarity
	Metal binding	305	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AF87 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: A334453B012DDCA0	FASTA	438	45,446
	10 20 30 40 50 60 MSDETFVITG AMLADGSRTD LLLAGGRIQE TGVRLSAAGA TVVDADGLLA LPGLVDLHTH 70 80 90 100 110 120 LREPGYEQSE TVLTGTRAAA AGGFTEVFAM ANTSPVADTA GVVEQVLSLG EAAGYATVRP 130 140 150 160 170 180 IGAVTVGLEG ERLAELGAMA DSRARVRVFS DDGKCVSDPL LMRRALEYVK AFDGVIAQHA 190 200 210 220 230 240 QEPRITAGAQ MNEGALSGEL GLAGWPAVAE ESIIARDVLL AEHVGSRLHV CHVSTAGSVD 250 260 270 280 290 300 VIRWAKARGI DVTAEVTPHH LLLTEELVSG YDARYKVNPP LRRSEDVEAL RAGLADGTID 310 320 330 340 350 360 IVATDHAPHP VEAKDCEWDA AAFGMVGLES ALSVVQSSVV DTGMLGWADI ARVLSATPAR 370 380 390 400 410 420 IGRLAGHGTP VEAGAPAELF LYDPAAAREF STGELAGKGV NSPYLSMTLS GRVVATFHRG 430 YATVLDGAVL ESVGGARG

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli." Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H. , Coutinho L.L., El-Dorry H.A., Ferro M.I.T., Gagliardi P.R., Giglioti E., Goldman M.H.S., Goldman G.H., Kimura E.T., Ferro E.S., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Mauro S.M.Z., Machado M.A., Marino C.L., Menck C.F., Nunes L.R., Oliveira R.C., Pereira G.G., Siqueira W., de Souza A.A., Tsai S.M., Zanca A.S., Simpson A.J.G., Brumbley S.M., Setubal J.C. Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CTCB07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016822 Genomic DNA. Translation: AAT88958.1.
RefSeq	YP_062063.1. NC_006087.1.
3D structure databases
ProteinModelPortal	Q6AF87.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2940482.
GenomeReviews	Gene locus Lxx11070 in contig AE016822_GR.
KEGG	lxx:Lxx11070.
NMPDR	fig|281090.3.peg.477.
PATRIC	22335788. VBILeiXyl11655_1144.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724623.
OMA	SHHQPHE.
ProtClustDB	PRK09357.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX11070-MONOMER.
Family and domain databases
HAMAP	MF_00220_B. PyrC_type2_B. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOase. IPR002195. Dihydroorotase_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR00857. PyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_LEIXX

Accession

Primary (citable) accession number: Q6AF87

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program