Q6AEY1 (DXR_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	Lxx12180

Organism

Leifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]

Taxonomic identifier

281090 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_0000163667
Regions
	Nucleotide binding	9 – 38	30	NADP By similarity
Sites
	Metal binding	126	1	Divalent metal cation By similarity
	Metal binding	128	1	Divalent metal cation By similarity
	Metal binding	197	1	Divalent metal cation By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AEY1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 3E050AA0F203CD50	FASTA	361	37,611
	10 20 30 40 50 60 MSVRRVVILG STGSIGMQAL EVVAANPDRF QVVGLTAGSS AGQLAEQAER FGVRETALGA 70 80 90 100 110 120 GDSERLVRSV DADVVLNGIT GSVGLGPTLA ALESGRTLAL ANKESLIVGG ELVKRAAAPG 130 140 150 160 170 180 QLIPVDSEHS ALAQALRSGA AGEVSRLVLT ASGGPFRGRS RESLRQVTPA EALAHPTWDM 190 200 210 220 230 240 GLVVTTNSST LVNKGLEVIE AHLLFGVPYD RIEVTVHPQS VVHSMVEFVD GSTIAQASPP 250 260 270 280 290 300 DMRLPISLGL GWPDRVPGTG LPLDWTRAHT WTFEPLDGEA FPAVALAKCV GVAGGTYPAV 310 320 330 340 350 360 FNAANEQAVA AFHAGAIGYL DIVDTVERVV DAHEPQAELT REGLAEAEGW ARSAADRMLG R

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli." Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H. , Coutinho L.L., El-Dorry H.A., Ferro M.I.T., Gagliardi P.R., Giglioti E., Goldman M.H.S., Goldman G.H., Kimura E.T., Ferro E.S., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Mauro S.M.Z., Machado M.A., Marino C.L., Menck C.F., Nunes L.R., Oliveira R.C., Pereira G.G., Siqueira W., de Souza A.A., Tsai S.M., Zanca A.S., Simpson A.J.G., Brumbley S.M., Setubal J.C. Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CTCB07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016822 Genomic DNA. Translation: AAT89064.1.
RefSeq	YP_062169.1. NC_006087.1.
3D structure databases
ProteinModelPortal	Q6AEY1.
SMR	Q6AEY1. Positions 4-360.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2940397.
GenomeReviews	Gene locus Lxx12180 in contig AE016822_GR.
KEGG	lxx:Lxx12180.
NMPDR	fig|281090.3.peg.686.
PATRIC	22336025. VBILeiXyl11655_1261.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG430762.
OMA	IHSMVEY.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX12180-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 2 hits. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_LEIXX

Accession

Primary (citable) accession number: Q6AEY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program