Q6AE56 (RSMH_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	Lxx15340

Organism

Leifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]

Taxonomic identifier

281090 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108648
Regions
	Region	41 – 43	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	60	1	S-adenosyl-L-methionine By similarity
	Binding site	87	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	108	1	S-adenosyl-L-methionine By similarity
	Binding site	115	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AE56 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 13CFA88B6502CA88	FASTA	325	35,515
	10 20 30 40 50 60 MSDDIHGDIH LPVLLERCIE LLAPALQEPG AVIVDATLGM GGHSAGILAR FPQATLVGLD 70 80 90 100 110 120 RDPDALAIAG ERLARFGDRV HLVHTVYDGI REALDGLGIA EVQGVLFDLG VSSLQLDRVE 130 140 150 160 170 180 RGFSYSKDAP LDMRMDGTSD LTAERVLAEY PEADLRRIFR DYGEEKLAAR YAQRIVAARQ 190 200 210 220 230 240 QGPITRSGEL VHLLQRATPA AIARQGHPAK RVFQALRIEV NQELAVLERA LPAAVDALAV 250 260 270 280 290 300 GGRLVVESYQ SLEDRIVKRE LQTRSRSSAP AGLPVELPEH RPELRLLIRG AELADDDEKA 310 320 ANPRAAPVRL RAAERTRASE DRRGS

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli." Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H. , Coutinho L.L., El-Dorry H.A., Ferro M.I.T., Gagliardi P.R., Giglioti E., Goldman M.H.S., Goldman G.H., Kimura E.T., Ferro E.S., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Mauro S.M.Z., Machado M.A., Marino C.L., Menck C.F., Nunes L.R., Oliveira R.C., Pereira G.G., Siqueira W., de Souza A.A., Tsai S.M., Zanca A.S., Simpson A.J.G., Brumbley S.M., Setubal J.C. Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CTCB07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016822 Genomic DNA. Translation: AAT89340.1.
RefSeq	YP_062445.1. NC_006087.1.
3D structure databases
ProteinModelPortal	Q6AE56.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2939576.
GenomeReviews	Gene locus Lxx15340 in contig AE016822_GR.
KEGG	lxx:Lxx15340.
NMPDR	fig|281090.3.peg.522.
PATRIC	22336706. VBILeiXyl11655_1595.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX15340-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_LEIXX

Accession

Primary (citable) accession number: Q6AE56

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program