Bifunctional enzyme IspD/IspF - Leifsonia xyli subsp. xyli (strain CTCB07)

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Q6ADI0 (ISPDF_LEIXX) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bifunctional enzyme IspD/IspF

Including the following 2 domains:

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Short name=MECDP-synthase
Short name=MECPS
EC=4.6.1.12

Gene names

Name:	ispDF
Synonyms:	ispF
Ordered Locus Names:	Lxx18250

Organism

Leifsonia xyli subsp. xyli (strain CTCB07) [Complete proteome] [HAMAP]

Taxonomic identifier

281090 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (IspF) By similarity. HAMAP MF_01520
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity. HAMAP MF_01520
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the IspD family. In the C-terminal section; belongs to the IspF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: EC 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 386

386

Bifunctional enzyme IspD/IspF HAMAP MF_01520

PRO_0000075671

Regions

Region

1 – 233

233

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520

Region

234 – 386

153

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520

Sites

Metal binding

240

Divalent metal cation By similarity

Metal binding

242

Divalent metal cation By similarity

Metal binding

274

Divalent metal cation By similarity

Site

Transition state stabilizer By similarity

Site

Transition state stabilizer By similarity

Site

160

Positions MEP for the nucleophilic attack By similarity

Site

213

Positions MEP for the nucleophilic attack By similarity

Site

266

Transition state stabilizer By similarity

Site

362

Transition state stabilizer By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6ADI0 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 8FE469E2AE70F185
Show »

FASTA

386

39,296

        10         20         30         40         50         60 
MCTASRPRIA VIVVAAGSGT RLGAGSPKAF VTLGGRTLLE RSLHAVRCMR EPAQVVVVAP 

        70         80         90        100        110        120 
EERLAEAAAL GEAGFGAAVD VVAGGETRQG SVAAGLGALR EGVEIVLVHD AARALTPAAQ 

       130        140        150        160        170        180 
FDAVVAAVDA GGAGVVPGLP VSDTIKRVGA DGEVRETVDR SVLSAVQTPQ GFPRDQLVAA 

       190        200        210        220        230        240 
YAAATTDETD DAELAAAAGH PVTVIPGDAR AFKITTPWDL RRAEELLAGL AAPRIGFGTD 

       250        260        270        280        290        300 
THAFDPSAEL WLAGLRWEGE PGLAGHSDGD VVAHAIVDAL LSAARLGDIG GVFGTGDPRF 

       310        320        330        340        350        360 
SGAHGEVFLT ETRRLVEEEG FRIGNVSAQI VGNRPKFAPR RAEAETLLSR VLGAPVSVSA 

       370        380 
TTTDGLGLTG RGEGVAVFAT ALLLAP

« Hide

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli."
Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H.

Setubal J.C.
Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CTCB07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016822 Genomic DNA. Translation: AAT89566.1.
RefSeq	YP_062671.1. NC_006087.1.
3D structure databases
ProteinModelPortal	Q6ADI0.
SMR	Q6ADI0. Positions 232-384.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2939630.
GenomeReviews	Gene locus Lxx18250 in contig AE016822_GR.
KEGG	lxx:Lxx18250.
NMPDR	fig\|281090.3.peg.1007.
PATRIC	22337308. VBILeiXyl11655_1892.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	IVLIHDA.
ProtClustDB	CLSK831416.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX18250-MONOMER.
Family and domain databases
HAMAP	MF_01520. IspDF. Divergent sequence. [Tree]
InterPro	IPR023423. IpsF_dom. IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase. IPR020555. MECDP_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
KO	K12506.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
SUPFAM	SSF69765. YgbB_synth. 1 hit.
TIGRFAMs	TIGR00453. IspD. 1 hit. TIGR00151. IspF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_LEIXX

Accession

Primary (citable) accession number: Q6ADI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents