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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Leifsonia xyli subsp. xyli (strain CTCB07)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201IronUniRule annotation
Metal bindingi124 – 1241IronUniRule annotation
Binding sitei182 – 1821SubstrateUniRule annotation
Binding sitei195 – 1951Substrate; via amide nitrogenUniRule annotation
Binding sitei199 – 1991SubstrateUniRule annotation
Binding sitei294 – 2941SubstrateUniRule annotation
Metal bindingi322 – 3221IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciLXYL281090:GH0X-1878-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:Lxx19910
OrganismiLeifsonia xyli subsp. xyli (strain CTCB07)
Taxonomic identifieri281090 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeLeifsonia
Proteomesi
  • UP000001306 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303406Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi281090.Lxx19910.

Structurei

3D structure databases

ProteinModelPortaliQ6AD38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1525Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiDHVHEVS.
OrthoDBiPOG091H010B.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AD38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRTASTEEG PLVLGIETSC DETGIGIVRG TTMLANVIAS SMEEHARYGG
60 70 80 90 100
VVPEVAARAH LEALTPALRT AVADAGIDLA AVDAVAVTSG PGLSGALMVG
110 120 130 140 150
VGAAKALALS LGKPLYAVNH LVGHVGADLL DADGGPGHPV ELPTVALLVS
160 170 180 190 200
GGHTSLLLVR DLVSDVELLG ETIDDAAGEA FDKVARLLGL PYPGGPQIDR
210 220 230 240 250
VAAAGDPRAI RFPRGLTKPQ DLERHRYDFS FSGVKTAVAR WVEKRQDAGE
260 270 280 290 300
EVPVADVAAS FREAVADVLL TKAIAACLDH GVPRLLLGGG VVANARVRQL
310 320 330 340 350
AAERAAAAGV ELRIPALSLC TDNGAMIAAL GAQLIMAGHE PSGLGFGADS
360
TLPVTDVHVV
Length:360
Mass (Da):36,719
Last modified:September 13, 2004 - v1
Checksum:iBDC0C588552CA9A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA. Translation: AAT89706.1.
RefSeqiWP_011186692.1. NC_006087.1.

Genome annotation databases

EnsemblBacteriaiAAT89706; AAT89706; Lxx19910.
KEGGilxx:Lxx19910.
PATRICi22337658. VBILeiXyl11655_2066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA. Translation: AAT89706.1.
RefSeqiWP_011186692.1. NC_006087.1.

3D structure databases

ProteinModelPortaliQ6AD38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi281090.Lxx19910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT89706; AAT89706; Lxx19910.
KEGGilxx:Lxx19910.
PATRICi22337658. VBILeiXyl11655_2066.

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiDHVHEVS.
OrthoDBiPOG091H010B.

Enzyme and pathway databases

BioCyciLXYL281090:GH0X-1878-MONOMER.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSAD_LEIXX
AccessioniPrimary (citable) accession number: Q6AD38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 13, 2004
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.