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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Leifsonia xyli subsp. xyli (strain CTCB07)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi120IronUniRule annotation1
Metal bindingi124IronUniRule annotation1
Binding sitei182SubstrateUniRule annotation1
Binding sitei195Substrate; via amide nitrogenUniRule annotation1
Binding sitei199SubstrateUniRule annotation1
Binding sitei294SubstrateUniRule annotation1
Metal bindingi322IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciLXYL281090:G1G06-2017-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:Lxx19910
OrganismiLeifsonia xyli subsp. xyli (strain CTCB07)
Taxonomic identifieri281090 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeLeifsonia
Proteomesi
  • UP000001306 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003034061 – 360tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST360

Interactioni

Protein-protein interaction databases

STRINGi281090.Lxx19910

Structurei

3D structure databases

ProteinModelPortaliQ6AD38
SMRiQ6AD38
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 152Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM Bacteria
COG0533 LUCA
HOGENOMiHOG000109568
KOiK01409
OMAiHLEGHIY
OrthoDBiPOG091H010B

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR017860 Peptidase_M22_CS
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit
PROSITEiView protein in PROSITE
PS01016 GLYCOPROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AD38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRTASTEEG PLVLGIETSC DETGIGIVRG TTMLANVIAS SMEEHARYGG
60 70 80 90 100
VVPEVAARAH LEALTPALRT AVADAGIDLA AVDAVAVTSG PGLSGALMVG
110 120 130 140 150
VGAAKALALS LGKPLYAVNH LVGHVGADLL DADGGPGHPV ELPTVALLVS
160 170 180 190 200
GGHTSLLLVR DLVSDVELLG ETIDDAAGEA FDKVARLLGL PYPGGPQIDR
210 220 230 240 250
VAAAGDPRAI RFPRGLTKPQ DLERHRYDFS FSGVKTAVAR WVEKRQDAGE
260 270 280 290 300
EVPVADVAAS FREAVADVLL TKAIAACLDH GVPRLLLGGG VVANARVRQL
310 320 330 340 350
AAERAAAAGV ELRIPALSLC TDNGAMIAAL GAQLIMAGHE PSGLGFGADS
360
TLPVTDVHVV
Length:360
Mass (Da):36,719
Last modified:September 13, 2004 - v1
Checksum:iBDC0C588552CA9A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016822 Genomic DNA Translation: AAT89706.1
RefSeqiWP_011186692.1, NC_006087.1

Genome annotation databases

EnsemblBacteriaiAAT89706; AAT89706; Lxx19910
KEGGilxx:Lxx19910

Similar proteinsi

Entry informationi

Entry nameiTSAD_LEIXX
AccessioniPrimary (citable) accession number: Q6AD38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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