Reviewed, UniProtKB/Swiss-Prot Q6ACE6 (PUR2_LEIXX)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Lxx22780

Organism

Leifsonia xyli subsp. xyli [Complete proteome] [HAMAP]

Taxonomic identifier

59736 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Leifsonia

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151457
Regions
	Domain	108 – 307	200	ATP-grasp
	Nucleotide binding	134 – 188	55	ATP By similarity
Sites
	Metal binding	277	1	Magnesium or manganese By similarity
	Metal binding	279	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ACE6-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 2A0F80CE0B2456C5	FASTA	417	43,135
	10 20 30 40 50 60 MKILVLGSGA REHAIITALL AERAGHDIVA APGNAGIAAD VAVEPGLDTL NGAAVTAFAI 70 80 90 100 110 120 ENGFELVVIG PEAPLVAGVA DPLRRRGIPV FGPGRAAAQL EGSKTFAKRI MDEAGVPTGR 130 140 150 160 170 180 AVRAATLAEA GSALDEFGAP YVVKADGLAA GKGVIVTEDR AAALAHAARY LTHGSVLVEE 190 200 210 220 230 240 FLDGEEVSLF LVSDGHTVLP LSPAQDYKRL LDGDAGPNTG GMGAYSPLTW LPETFVDEVI 250 260 270 280 290 300 DTVAMPTVRQ LAAEQTPFIG LLYCGLIVTA KGIRVIEFNA RFGDPETQVV LPRLVTPLST 310 320 330 340 350 360 LLFAAATGTL SGMPRPEFAP GTTVTVVLAS EDYPESPRTG RPIAGLDEAA SVPEVTIAHA 370 380 390 400 410 STARDETTGA LLATGGRVLS VVARGATFAD ARTRAYDALS RIRLDGSQYR TDIAARA

References

[1]

"The genome sequence of the Gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli." Monteiro-Vitorello C.B., Camargo L.E.A., Van Sluys M.A., Kitajima J.P., Truffi D., do Amaral A.M., Harakava R., de Oliveira J.C.F., Wood D., de Oliveira M.C., Miyaki C.Y., Takita M.A., da Silva A.C.R., Furlan L.R., Carraro D.M., Camarotte G., Almeida N.F. Jr., Carrer H. , Coutinho L.L., El-Dorry H.A., Ferro M.I.T., Gagliardi P.R., Giglioti E., Goldman M.H.S., Goldman G.H., Kimura E.T., Ferro E.S., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Mauro S.M.Z., Machado M.A., Marino C.L., Menck C.F., Nunes L.R., Oliveira R.C., Pereira G.G., Siqueira W., de Souza A.A., Tsai S.M., Zanca A.S., Simpson A.J.G., Brumbley S.M., Setubal J.C. Mol. Plant Microbe Interact. 17:827-836(2004) [PubMed: 15305603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CTCB07.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016822 Genomic DNA. Translation: AAT89947.1.
RefSeq	YP_063052.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2939853.
GenomeReviews	Gene locus Lxx22780 in contig AE016822_GR.
KEGG	lxx:Lxx22780.
NMPDR	fig|281090.3.peg.1839.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6ACE6.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	LXYL281090:LXX22780-MON.
BRENDA	6.3.4.13. 297084.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_LEIXX

Accession

Primary (citable) accession number: Q6ACE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	September 13, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)