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Q6AB89

- SYI_PROAC

UniProt

Q6AB89 - SYI_PROAC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Propionibacterium acnes (strain KPA171202 / DSM 16379)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei643 – 6431ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPACN267747:GHO9-219-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PPA0216
    OrganismiPropionibacterium acnes (strain KPA171202 / DSM 16379)
    Taxonomic identifieri267747 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium
    ProteomesiUP000000603: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11141114Isoleucine--tRNA ligasePRO_0000098555Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi267747.PPA0216.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6AB89.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 7111"HIGH" regionAdd
    BLAST
    Motifi640 – 6445"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiPRFKTMK.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6AB89-1 [UniParc]FASTAAdd to Basket

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    MTDVNPVRSD NCRQYNNVPP QIDLPAMDHE IIDLWARQHT FDKSLEATKD     50
    GQPWTFFEGP PTANGQPGTH HVEARVFKDI FPRFKTMQGF RVDRKAGWDC 100
    HGLPVELAVE KELGFSGKPD IEKYGVEPFN AKCRESVTRH VDAFSELTER 150
    MGYWVNMDEA YWTMSPSYVE SVWWGLKRIW DKGLLGEDHR VAPYCPRCGT 200
    TLSDHELAQG YQDDRDPSIY VRFPVTSGPL AGRAKLLVWT TTPWTLVSNT 250
    AVAVHPEVRY VVAHRDPVPE GSDAVATASA EDPASQDLII AEPLFEKVLG 300
    EGWSLTGESF LGSQMELWTY ERPYNFLEWP KTERVTVDGR PTPADANFVV 350
    LADYVTVEDG TGLVHQAPAF GADDLQTCRR YGLPLVNPIR PDGTFEESVP 400
    LVGGQFFKTA DKPLCEDLDR RGVLFRLEMH WHSYPHCWRC DTHLIYYAQP 450
    SWYIRTTKVK EQLLAQNEVT TWYPETIKHG RFGDWLENNI DWAVSRSRYW 500
    GTPLPLWRND DDRSDVICVE SLAELSQYVG RDLTGMDPHR PFIDEVTFTR 550
    DGHTYHRVPE VADAWLDSGS MPFAQWGYPH VPGSKEKFES HYPGDFICEA 600
    IDQTRGWFYT MMAVGTLVFD ESSYRNVLCL GHILAEDGRK MSKHLGNILL 650
    PIPLMDSHGA DALRWFMAAD GSPWSARRVG DETIQETVRK VLLTYWNTVS 700
    FQVLYARANG WSPAQGTQHD ADPARGFGGV VPPAVGSRGF GGVVPPAVTE 750
    RHVLDRWLVS ATNVLVRDVT EALNNFDTQR VGNLIAQFVD ELSNWYVRRS 800
    RRRFWDGDEG ALWTLHETLE TLTKLMAPMV PFITERVWQD LFVTTNPHGP 850
    ESVHLASWPV ADDSLIDESL SESMDLARRI VELGRGARAE AKAKIRQPLS 900
    RALISGAALA KLDEDLQAEI RSELNVMALD SFTAAGDLVD HCAKGNFRAL 950
    GKKYAKATPK VAAAIAAADP EWLASELAIK GSVEMDVPEV EGGKAVVTAD 1000
    DVIVSERPRE GWSVVNEQGE TVALDLEITP ELARAGQARE VIRFVQDSRK 1050
    KAGLDVSDRI TLAWSASADL ATAIEEHAEQ ISQEVLAVQM SREPRADDWA 1100
    VEPDLGLAVK VVKV 1114
    Length:1,114
    Mass (Da):124,981
    Last modified:September 13, 2004 - v1
    Checksum:i22FBA25983231947
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017283 Genomic DNA. Translation: AAT81977.1.
    RefSeqiYP_054935.1. NC_006085.1.

    Genome annotation databases

    EnsemblBacteriaiAAT81977; AAT81977; PPA0216.
    GeneIDi2932887.
    KEGGipac:PPA0216.
    PATRICi23035081. VBIProAcn64440_0226.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017283 Genomic DNA. Translation: AAT81977.1 .
    RefSeqi YP_054935.1. NC_006085.1.

    3D structure databases

    ProteinModelPortali Q6AB89.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267747.PPA0216.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT81977 ; AAT81977 ; PPA0216 .
    GeneIDi 2932887.
    KEGGi pac:PPA0216.
    PATRICi 23035081. VBIProAcn64440_0226.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi PRFKTMK.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PACN267747:GHO9-219-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Propionibacterium acnes, a commensal of human skin."
      Brueggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Duerre P., Gottschalk G.
      Science 305:671-673(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KPA171202 / DSM 16379.

    Entry informationi

    Entry nameiSYI_PROAC
    AccessioniPrimary (citable) accession number: Q6AB89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3