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Q6AB89

- SYI_PROAC

UniProt

Q6AB89 - SYI_PROAC

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PPA0216
Organism
Propionibacterium acnes (strain KPA171202 / DSM 16379)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei643 – 6431ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPACN267747:GHO9-219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PPA0216
OrganismiPropionibacterium acnes (strain KPA171202 / DSM 16379)
Taxonomic identifieri267747 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium
ProteomesiUP000000603: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11141114Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098555Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi267747.PPA0216.

Structurei

3D structure databases

ProteinModelPortaliQ6AB89.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionUniRule annotation
Add
BLAST
Motifi640 – 6445"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiPRFKTMK.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AB89-1 [UniParc]FASTAAdd to Basket

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MTDVNPVRSD NCRQYNNVPP QIDLPAMDHE IIDLWARQHT FDKSLEATKD     50
GQPWTFFEGP PTANGQPGTH HVEARVFKDI FPRFKTMQGF RVDRKAGWDC 100
HGLPVELAVE KELGFSGKPD IEKYGVEPFN AKCRESVTRH VDAFSELTER 150
MGYWVNMDEA YWTMSPSYVE SVWWGLKRIW DKGLLGEDHR VAPYCPRCGT 200
TLSDHELAQG YQDDRDPSIY VRFPVTSGPL AGRAKLLVWT TTPWTLVSNT 250
AVAVHPEVRY VVAHRDPVPE GSDAVATASA EDPASQDLII AEPLFEKVLG 300
EGWSLTGESF LGSQMELWTY ERPYNFLEWP KTERVTVDGR PTPADANFVV 350
LADYVTVEDG TGLVHQAPAF GADDLQTCRR YGLPLVNPIR PDGTFEESVP 400
LVGGQFFKTA DKPLCEDLDR RGVLFRLEMH WHSYPHCWRC DTHLIYYAQP 450
SWYIRTTKVK EQLLAQNEVT TWYPETIKHG RFGDWLENNI DWAVSRSRYW 500
GTPLPLWRND DDRSDVICVE SLAELSQYVG RDLTGMDPHR PFIDEVTFTR 550
DGHTYHRVPE VADAWLDSGS MPFAQWGYPH VPGSKEKFES HYPGDFICEA 600
IDQTRGWFYT MMAVGTLVFD ESSYRNVLCL GHILAEDGRK MSKHLGNILL 650
PIPLMDSHGA DALRWFMAAD GSPWSARRVG DETIQETVRK VLLTYWNTVS 700
FQVLYARANG WSPAQGTQHD ADPARGFGGV VPPAVGSRGF GGVVPPAVTE 750
RHVLDRWLVS ATNVLVRDVT EALNNFDTQR VGNLIAQFVD ELSNWYVRRS 800
RRRFWDGDEG ALWTLHETLE TLTKLMAPMV PFITERVWQD LFVTTNPHGP 850
ESVHLASWPV ADDSLIDESL SESMDLARRI VELGRGARAE AKAKIRQPLS 900
RALISGAALA KLDEDLQAEI RSELNVMALD SFTAAGDLVD HCAKGNFRAL 950
GKKYAKATPK VAAAIAAADP EWLASELAIK GSVEMDVPEV EGGKAVVTAD 1000
DVIVSERPRE GWSVVNEQGE TVALDLEITP ELARAGQARE VIRFVQDSRK 1050
KAGLDVSDRI TLAWSASADL ATAIEEHAEQ ISQEVLAVQM SREPRADDWA 1100
VEPDLGLAVK VVKV 1114
Length:1,114
Mass (Da):124,981
Last modified:September 13, 2004 - v1
Checksum:i22FBA25983231947
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017283 Genomic DNA. Translation: AAT81977.1.
RefSeqiYP_054935.1. NC_006085.1.

Genome annotation databases

EnsemblBacteriaiAAT81977; AAT81977; PPA0216.
GeneIDi2932887.
KEGGipac:PPA0216.
PATRICi23035081. VBIProAcn64440_0226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017283 Genomic DNA. Translation: AAT81977.1 .
RefSeqi YP_054935.1. NC_006085.1.

3D structure databases

ProteinModelPortali Q6AB89.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 267747.PPA0216.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT81977 ; AAT81977 ; PPA0216 .
GeneIDi 2932887.
KEGGi pac:PPA0216.
PATRICi 23035081. VBIProAcn64440_0226.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi PRFKTMK.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PACN267747:GHO9-219-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Propionibacterium acnes, a commensal of human skin."
    Brueggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Duerre P., Gottschalk G.
    Science 305:671-673(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KPA171202 / DSM 16379.

Entry informationi

Entry nameiSYI_PROAC
AccessioniPrimary (citable) accession number: Q6AB89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2004
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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