Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6AB89

- SYI_PROAC

UniProt

Q6AB89 - SYI_PROAC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Propionibacterium acnes (strain KPA171202 / DSM 16379)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei643 – 6431ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPACN267747:GHO9-219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PPA0216
OrganismiPropionibacterium acnes (strain KPA171202 / DSM 16379)
Taxonomic identifieri267747 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium
ProteomesiUP000000603: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11141114Isoleucine--tRNA ligasePRO_0000098555Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi267747.PPA0216.

Structurei

3D structure databases

ProteinModelPortaliQ6AB89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionAdd
BLAST
Motifi640 – 6445"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiPRFKTMK.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AB89 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDVNPVRSD NCRQYNNVPP QIDLPAMDHE IIDLWARQHT FDKSLEATKD
60 70 80 90 100
GQPWTFFEGP PTANGQPGTH HVEARVFKDI FPRFKTMQGF RVDRKAGWDC
110 120 130 140 150
HGLPVELAVE KELGFSGKPD IEKYGVEPFN AKCRESVTRH VDAFSELTER
160 170 180 190 200
MGYWVNMDEA YWTMSPSYVE SVWWGLKRIW DKGLLGEDHR VAPYCPRCGT
210 220 230 240 250
TLSDHELAQG YQDDRDPSIY VRFPVTSGPL AGRAKLLVWT TTPWTLVSNT
260 270 280 290 300
AVAVHPEVRY VVAHRDPVPE GSDAVATASA EDPASQDLII AEPLFEKVLG
310 320 330 340 350
EGWSLTGESF LGSQMELWTY ERPYNFLEWP KTERVTVDGR PTPADANFVV
360 370 380 390 400
LADYVTVEDG TGLVHQAPAF GADDLQTCRR YGLPLVNPIR PDGTFEESVP
410 420 430 440 450
LVGGQFFKTA DKPLCEDLDR RGVLFRLEMH WHSYPHCWRC DTHLIYYAQP
460 470 480 490 500
SWYIRTTKVK EQLLAQNEVT TWYPETIKHG RFGDWLENNI DWAVSRSRYW
510 520 530 540 550
GTPLPLWRND DDRSDVICVE SLAELSQYVG RDLTGMDPHR PFIDEVTFTR
560 570 580 590 600
DGHTYHRVPE VADAWLDSGS MPFAQWGYPH VPGSKEKFES HYPGDFICEA
610 620 630 640 650
IDQTRGWFYT MMAVGTLVFD ESSYRNVLCL GHILAEDGRK MSKHLGNILL
660 670 680 690 700
PIPLMDSHGA DALRWFMAAD GSPWSARRVG DETIQETVRK VLLTYWNTVS
710 720 730 740 750
FQVLYARANG WSPAQGTQHD ADPARGFGGV VPPAVGSRGF GGVVPPAVTE
760 770 780 790 800
RHVLDRWLVS ATNVLVRDVT EALNNFDTQR VGNLIAQFVD ELSNWYVRRS
810 820 830 840 850
RRRFWDGDEG ALWTLHETLE TLTKLMAPMV PFITERVWQD LFVTTNPHGP
860 870 880 890 900
ESVHLASWPV ADDSLIDESL SESMDLARRI VELGRGARAE AKAKIRQPLS
910 920 930 940 950
RALISGAALA KLDEDLQAEI RSELNVMALD SFTAAGDLVD HCAKGNFRAL
960 970 980 990 1000
GKKYAKATPK VAAAIAAADP EWLASELAIK GSVEMDVPEV EGGKAVVTAD
1010 1020 1030 1040 1050
DVIVSERPRE GWSVVNEQGE TVALDLEITP ELARAGQARE VIRFVQDSRK
1060 1070 1080 1090 1100
KAGLDVSDRI TLAWSASADL ATAIEEHAEQ ISQEVLAVQM SREPRADDWA
1110
VEPDLGLAVK VVKV
Length:1,114
Mass (Da):124,981
Last modified:September 13, 2004 - v1
Checksum:i22FBA25983231947
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017283 Genomic DNA. Translation: AAT81977.1.
RefSeqiYP_054935.1. NC_006085.1.

Genome annotation databases

EnsemblBacteriaiAAT81977; AAT81977; PPA0216.
GeneIDi2932887.
KEGGipac:PPA0216.
PATRICi23035081. VBIProAcn64440_0226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017283 Genomic DNA. Translation: AAT81977.1 .
RefSeqi YP_054935.1. NC_006085.1.

3D structure databases

ProteinModelPortali Q6AB89.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 267747.PPA0216.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT81977 ; AAT81977 ; PPA0216 .
GeneIDi 2932887.
KEGGi pac:PPA0216.
PATRICi 23035081. VBIProAcn64440_0226.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi PRFKTMK.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PACN267747:GHO9-219-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Propionibacterium acnes, a commensal of human skin."
    Brueggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Duerre P., Gottschalk G.
    Science 305:671-673(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KPA171202 / DSM 16379.

Entry informationi

Entry nameiSYI_PROAC
AccessioniPrimary (citable) accession number: Q6AB89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2004
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3