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Q6A9B0 (MEND_PROAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:PPA0903
OrganismPropionibacterium acnes (strain KPA171202 / DSM 16379) [Complete proteome] [HAMAP]
Taxonomic identifier267747 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence caution

The sequence AAT82656.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5085082-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_0000341809

Sequences

Sequence LengthMass (Da)Tools
Q6A9B0 [UniParc].

Last modified July 1, 2008. Version 2.
Checksum: B9ADC09A593370E0

FASTA50853,075
        10         20         30         40         50         60 
MNPSTAVARR LVNALVEAGV EHVVYCPGSR DAPIGYALAD AETAGWLRVY VRLDERSAGF 

        70         80         90        100        110        120 
VALGLGRAGC PAALVTTSGT AVANIHPAVL EADAAGVPLI VLSADRPAEM WHTGANQTTV 

       130        140        150        160        170        180 
QTGIFGSAPR LSTDVPAGFP ADERLDALVL RAVTAATGAL SADPGPVHLN VSFRDSLVPD 

       190        200        210        220        230        240 
GPWQPQALVP RRVSSFPTAP TPLVMPARTV VVAGDGAGSL ARELAQQGGW PLLAEPTSGS 

       250        260        270        280        290        300 
RVGDNALTDY QTVLGSELVD DVEAVLVLGH PTLSRPVSRL LARPDVTVVT DRSRWTDVAG 

       310        320        330        340        350        360 
VARVVTGPVE LAEIDTDPAW LGRWKDADRP VVPTAKQRLC RDIWWACTTP NAPVLVIGAS 

       370        380        390        400        410        420 
EVIRCFDRFA VPGDIAPTAL ANRGLAGIDG TIATAIGVGL GTGRPVRVVV GDLTFAHDAM 

       430        440        450        460        470        480 
SLLLGETEPE PDVQVVVLDD RGGAIFSGLE HAAAPAPVLR RMFLTPQRLD SAALAHALGA 

       490        500 
SHRRVSPDDL QFLDEPVVGR QVVSVPLT 

« Hide

References

[1]"The complete genome sequence of Propionibacterium acnes, a commensal of human skin."
Brueggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Duerre P., Gottschalk G.
Science 305:671-673(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KPA171202 / DSM 16379.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017283 Genomic DNA. Translation: AAT82656.1. Different initiation.
RefSeqYP_055614.1. NC_006085.1.

3D structure databases

ProteinModelPortalQ6A9B0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267747.PPA0903.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT82656; AAT82656; PPA0903.
GeneID2930918.
KEGGpac:PPA0903.
PATRIC23036523. VBIProAcn64440_0936.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218359.
KOK02551.
OMAWRSAVCR.
OrthoDBEOG6NWBQW.
ProtClustDBCLSK2763589.

Enzyme and pathway databases

BioCycPACN267747:GHO9-912-MONOMER.
UniPathwayUPA00079; UER00164.

Family and domain databases

HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_PROAC
AccessionPrimary (citable) accession number: Q6A9B0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: February 19, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways