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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Propionibacterium acnes (strain KPA171202 / DSM 16379)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80NucleophileUniRule annotation1
Binding sitei109L-glutamineUniRule annotation1
Active sitei180Charge relay systemUniRule annotation1
Active sitei182Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:PPA0966
OrganismiPropionibacterium acnes (strain KPA171202 / DSM 16379)
Taxonomic identifieri267747 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesPropionibacteriaceaePropionibacterium
Proteomesi
  • UP000000603 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001356541 – 201Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST201

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi267747.PPA0966.

Structurei

3D structure databases

ProteinModelPortaliQ6A947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 50L-glutamine bindingUniRule annotation3
Regioni137 – 138L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UHX. Bacteria.
COG0311. LUCA.
HOGENOMiHOG000039949.
KOiK08681.
OMAiVYGTCAG.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
[Graphical view]
PANTHERiPTHR31559:SF0. PTHR31559:SF0. 1 hit.
PfamiPF01174. SNO. 1 hit.
[Graphical view]
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiPS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6A947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPLVGVVAL QGGFAEHIEV LESLGANTRR VRRSADLQGL DGIVLPGGES
60 70 80 90 100
TVIDKLMRSF SLAEPLKDAV RRGLPVLATC AGLVVLATDL EDAAKGQHTL
110 120 130 140 150
SLLDVTVRRN AFGSQLDSFE GTLDIDGVGD GVSATFIRAP VITRVGPGVE
160 170 180 190 200
VIAQLPDEAG NVSGAIVGVR QRNVLALSFH PEETDDDRVH RTWLRQVSEE

V
Length:201
Mass (Da):21,324
Last modified:September 13, 2004 - v1
Checksum:i1588DDE933E5AAC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017283 Genomic DNA. Translation: AAT82719.1.

Genome annotation databases

EnsemblBacteriaiAAT82719; AAT82719; PPA0966.
KEGGipac:PPA0966.
PATRICi23036655. VBIProAcn64440_1001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017283 Genomic DNA. Translation: AAT82719.1.

3D structure databases

ProteinModelPortaliQ6A947.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267747.PPA0966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT82719; AAT82719; PPA0966.
KEGGipac:PPA0966.
PATRICi23036655. VBIProAcn64440_1001.

Phylogenomic databases

eggNOGiENOG4108UHX. Bacteria.
COG0311. LUCA.
HOGENOMiHOG000039949.
KOiK08681.
OMAiVYGTCAG.

Enzyme and pathway databases

UniPathwayiUPA00245.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
[Graphical view]
PANTHERiPTHR31559:SF0. PTHR31559:SF0. 1 hit.
PfamiPF01174. SNO. 1 hit.
[Graphical view]
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiPS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDXT_PROAC
AccessioniPrimary (citable) accession number: Q6A947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.