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Protein

Chorismate synthase

Gene

aroC

Organism
Propionibacterium acnes (strain KPA171202 / DSM 16379)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401NADPUniRule annotation
Binding sitei46 – 461NADPUniRule annotation
Binding sitei300 – 3001FMN; via amide nitrogenUniRule annotation
Binding sitei341 – 3411FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 1373FMNUniRule annotation
Nucleotide bindingi256 – 2572FMNUniRule annotation
Nucleotide bindingi315 – 3195FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciPACN267747:GHO9-1195-MONOMER.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Ordered Locus Names:PPA1182
OrganismiPropionibacterium acnes (strain KPA171202 / DSM 16379)
Taxonomic identifieri267747 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesPropionibacteriaceaePropionibacterium
Proteomesi
  • UP000000603 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Chorismate synthasePRO_0000140627Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi267747.PPA1182.

Structurei

3D structure databases

ProteinModelPortaliQ6A8I4.
SMRiQ6A8I4. Positions 1-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6A8I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRYLTAGES HGPALVATME GIPAHVQVGA VEISEELRRR RLGAGRGARM
60 70 80 90 100
SFEADELELL AGFRHGETLG DPLAIRIGNS EWEKWRTVMA PGPVAPEDLA
110 120 130 140 150
GHARAAALTR PRPGHADLAG MQKYDFDEAR PILERASARE TAARVALGAI
160 170 180 190 200
AKAFLKQSLG IEVLSHVVSL GPVSAPNGPR PDPSDLTRID ADPVRCADSV
210 220 230 240 250
TSKRMVAEIE ACRKEGDTLG GVVEVLAYGL PAGLGSHSQG DRRLDARLAG
260 270 280 290 300
ALMGIQAIKG VEFGDGFDLT RRRGSQAHDW MEPTEGGIHR VSDRAGGTEG
310 320 330 340 350
GMSTGQILRV RAAMKPIATV PRALPTVDVT TGEAANAHHQ RSDVCAVPAA
360 370 380 390
GVVAEAMAAL VLAECALEKF GGDSVTETRR NAQAYLDRLA ARGLAVAP
Length:398
Mass (Da):41,931
Last modified:September 13, 2004 - v1
Checksum:i356D822C64B0E2B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017283 Genomic DNA. Translation: AAT82931.1.
RefSeqiWP_002517990.1. NC_006085.1.

Genome annotation databases

EnsemblBacteriaiAAT82931; AAT82931; PPA1182.
GeneIDi2931316.
KEGGipac:PPA1182.
PATRICi23037099. VBIProAcn64440_1218.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017283 Genomic DNA. Translation: AAT82931.1.
RefSeqiWP_002517990.1. NC_006085.1.

3D structure databases

ProteinModelPortaliQ6A8I4.
SMRiQ6A8I4. Positions 1-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267747.PPA1182.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT82931; AAT82931; PPA1182.
GeneIDi2931316.
KEGGipac:PPA1182.
PATRICi23037099. VBIProAcn64440_1218.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciPACN267747:GHO9-1195-MONOMER.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Propionibacterium acnes, a commensal of human skin."
    Brueggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Duerre P., Gottschalk G.
    Science 305:671-673(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KPA171202 / DSM 16379.

Entry informationi

Entry nameiAROC_PROAC
AccessioniPrimary (citable) accession number: Q6A8I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: September 13, 2004
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.