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Q6A7V7 (BIOB_PROAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:PPA1407
OrganismPropionibacterium acnes (strain KPA171202 / DSM 16379) [Complete proteome] [HAMAP]
Taxonomic identifier267747 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence AAT83158.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Biotin synthase HAMAP-Rule MF_01694
PRO_0000381535

Sites

Metal binding591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1031Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1361Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2671Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6A7V7 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: BD661CA1E0BF2183

FASTA35838,940
        10         20         30         40         50         60 
MTERGLRGES ITREEALEIL RSSDDELMSI IAAAGKVRRH FFDNRVRLNY LVNLKSGLCP 

        70         80         90        100        110        120 
EDCSYCSQRL GSRAEIMKYS WADPQKVHDA VEAGIAGGAR RVCMVASGHG PSRRDVERVN 

       130        140        150        160        170        180 
GMVRSLKADH PDVEVCVCLG FVDDEKAASI KEAGADAYNH NANTARSHYG KICSTHSYED 

       190        200        210        220        230        240 
RMDTVEVLKR NGLSPCSGVI AGMGETDEEF VDVIFDLRKH GVDSVPVNFL LPFEGTPLAG 

       250        260        270        280        290        300 
GAQHITPQWC LKRLAMVRFA HPDSEVRAAA GREQHIRTMQ PLALEVVNSI FLGDYLTSEG 

       310        320        330        340        350 
AAGAADLQMI EDGGFIPEGA DGQPMVHTDV NSHHSANLPV NAVPIRHRGI GTEVPANA 

« Hide

References

[1]"The complete genome sequence of Propionibacterium acnes, a commensal of human skin."
Brueggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Duerre P., Gottschalk G.
Science 305:671-673(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KPA171202 / DSM 16379.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017283 Genomic DNA. Translation: AAT83158.1. Different initiation.
RefSeqYP_056116.1. NC_006085.1.

3D structure databases

ProteinModelPortalQ6A7V7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267747.PPA1407.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT83158; AAT83158; PPA1407.
GeneID2930948.
KEGGpac:PPA1407.
PATRIC23037585. VBIProAcn64440_1452.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMATSEATYG.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycPACN267747:GHO9-1432-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PROAC
AccessionPrimary (citable) accession number: Q6A7V7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways