Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Cutibacterium acnes (strain DSM 16379 / KPA171202) (Propionibacterium acnes)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotation

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.Curated

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 4 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (PPA2148)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei131Proton donor/acceptorUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Active sitei135Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 13NAD(P)UniRule annotation6
Nucleotide bindingi74 – 76NAD(P)UniRule annotation3
Nucleotide bindingi101 – 104NAD(P)UniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotation (EC:1.17.1.8UniRule annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:PPA1470
OrganismiCutibacterium acnes (strain DSM 16379 / KPA171202) (Propionibacterium acnes)
Taxonomic identifieri267747 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesPropionibacteriaceaeCutibacterium
Proteomesi
  • UP000000603 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002283721 – 2464-hydroxy-tetrahydrodipicolinate reductaseAdd BLAST246

Interactioni

Protein-protein interaction databases

STRINGi267747.PPA1470

Structurei

3D structure databases

ProteinModelPortaliQ6A7P7
SMRiQ6A7P7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 142Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DapB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DUK Bacteria
COG0289 LUCA
HOGENOMiHOG000227154
KOiK00215
OMAiRESFMPG

Family and domain databases

HAMAPiMF_00102 DapB, 1 hit
InterProiView protein in InterPro
IPR022663 DapB_C
IPR000846 DapB_N
IPR022664 DapB_N_CS
IPR023940 DHDPR_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR20836 PTHR20836, 1 hit
PfamiView protein in Pfam
PF05173 DapB_C, 1 hit
PF01113 DapB_N, 1 hit
PIRSFiPIRSF000161 DHPR, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00036 dapB, 1 hit
PROSITEiView protein in PROSITE
PS01298 DAPB, 1 hit

Sequencei

Sequence statusi: Complete.

Q6A7P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVAVFGAK GRMGTAVCQA VEEAEDTELV AAVDSGGDRS DASGADVIVD
60 70 80 90 100
FTTPDAVMDN LSWAISHDIN TVVGTTGFDD ERYQVLRDQL ADHPNIGCLV
110 120 130 140 150
APNFSIGAVL MMHFAEQAAR FYESAEIVEL HHPNKVDAPS GTARTTATKI
160 170 180 190 200
AAARQEAGLG EVPDATKSQL NGARGAVVEG VHVHSVRLRG LVAHQEVLFG
210 220 230 240
AEGETLTIRH DSMDRVSFMS GVLTGVRGVL DRPGLTVGIE GLLGLE
Length:246
Mass (Da):25,944
Last modified:September 13, 2004 - v1
Checksum:i065B38074FA84D8C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017283 Genomic DNA Translation: AAT83218.1
RefSeqiWP_002516882.1, NC_006085.1

Genome annotation databases

EnsemblBacteriaiAAT83218; AAT83218; PPA1470
GeneIDi2931618
KEGGipac:PPA1470

Similar proteinsi

Entry informationi

Entry nameiDAPB_CUTAK
AccessioniPrimary (citable) accession number: Q6A7P7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: September 13, 2004
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health