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Protein

Thymidylate synthase

Gene

thyA

Organism
Cutibacterium acnes (strain DSM 16379 / KPA171202) (Propionibacterium acnes)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Active sitei145NucleophileUniRule annotation1
Binding sitei1745,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei182dUMPUniRule annotation1
Binding sitei2685,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi125 – 126dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi171 – 174dUMPUniRule annotation4
Nucleotide bindingi212 – 214dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:PPA1672
OrganismiCutibacterium acnes (strain DSM 16379 / KPA171202) (Propionibacterium acnes)
Taxonomic identifieri267747 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesPropionibacteriaceaeCutibacterium
Proteomesi
  • UP000000603 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410031 – 269Thymidylate synthaseAdd BLAST269

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi267747.PPA1672

Structurei

3D structure databases

ProteinModelPortaliQ6A761
SMRiQ6A761
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257899
KOiK00560
OMAiKQYLDLC

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 2 hits
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q6A761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIYLDLLRR ILDEGVRRED RTGTGTVSVF GHQMVFDLRE GFPLVTTKKI
60 70 80 90 100
YTRSVFGELL WFLRGDTNVG WLHDNNIHIW DEWADENGDL GPVYGHQWRS
110 120 130 140 150
WPDDDGTIDQ IANVVEQIRT NPWSRRHIVS AWNVAEIKEM ALPPCHTLFQ
160 170 180 190 200
FYVTPDDRGT PTWLSCQLYQ RSGDTFLGVP FNIASYALLT HLVASVTGLK
210 220 230 240 250
PLRFVHTLGD AHVYLNHLDQ VHEQLKRKPR HRPTLTVNPG IRDIDGFELS
260
DITLTGYDPY PALPAPIAV
Length:269
Mass (Da):30,838
Last modified:September 13, 2004 - v1
Checksum:i80AFC40F5B687168
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017283 Genomic DNA Translation: AAT83404.1
RefSeqiWP_002514671.1, NC_006085.1

Genome annotation databases

EnsemblBacteriaiAAT83404; AAT83404; PPA1672
GeneIDi2933035
KEGGipac:PPA1672

Similar proteinsi

Entry informationi

Entry nameiTYSY_CUTAK
AccessioniPrimary (citable) accession number: Q6A761
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: September 13, 2004
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health