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Q6A6S9 (TRMB_PROAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:PPA1808
OrganismPropionibacterium acnes (strain KPA171202 / DSM 16379) [Complete proteome] [HAMAP]
Taxonomic identifier267747 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Propionibacteriaceae › Propionibacterium ›

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Sequence caution

The sequence AAT83534.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 276276tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171372

Regions

Region252 – 2554Substrate binding Potential

Sites

Binding site1031S-adenosyl-L-methionine By similarity
Binding site1281S-adenosyl-L-methionine By similarity
Binding site1551S-adenosyl-L-methionine By similarity
Binding site1781S-adenosyl-L-methionine By similarity
Binding site1821Substrate By similarity
Binding site2141Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q6A6S9 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 8695F58D362F154D

FASTA27630,383
        10         20         30         40         50         60 
MRPDPAPLDP TDASPAQARR HQPGLGVEVS AGAHRSGDRV RRGVVSFVRR SPRMNVSQQR 

        70         80         90        100        110        120 
AMNTLASTYL IDVPRDATST SVAPGSRLDL PAIFGRTAPL TVEIGVGSGD VLAALAAAHP 

       130        140        150        160        170        180 
ERDFIGFEVY LPSIATTLNK LENAGASNAR VIMADATAGL DHLFGPADLD ELWTFFADPW 

       190        200        210        220        230        240 
HKKRHHKRRI VNPDTARLVT SRLRPGGLWR LATDWDDYAH WMLEVLSAEP LLKPVDAGPD 

       250        260        270 
GFSPRWPERP VTRYENKGLT AGRTIHDLTW RRVDES

« Hide

References

[1]"The complete genome sequence of Propionibacterium acnes, a commensal of human skin."
Brueggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Duerre P., Gottschalk G.
Science 305:671-673(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KPA171202 / DSM 16379.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017283 Genomic DNA. Translation: AAT83534.1. Different initiation.
RefSeqYP_056492.1. NC_006085.1.

3D structure databases

ProteinModelPortalQ6A6S9.
ModBaseSearch...

Protein-protein interaction databases

STRING267747.PPA1808.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT83534; AAT83534; PPA1808.
GeneID2931436.
KEGGpac:PPA1808.
PATRIC23038433. VBIProAcn64440_1866.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMACEVHEPG.
ProtClustDBCLSK2764190.

Enzyme and pathway databases

BioCycPACN267747:GHO9-1839-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_PROAC
AccessionPrimary (citable) accession number: Q6A6S9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: May 29, 2007
Last modified: May 1, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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