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Protein

Thioredoxin domain-containing protein 8

Gene

TXNDC8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be required for post-translational modifications of proteins required for acrosomal biogenesis. May act by reducing disulfide bonds within the sperm.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin domain-containing protein 8
Alternative name(s):
Spermatid-specific thioredoxin-3
Short name:
Sptrx-3
Thioredoxin-6
Gene namesi
Name:TXNDC8
Synonyms:SPTRX3, TRX6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:31454. TXNDC8.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: Ensembl
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • mitochondrion Source: GO_Central
  • sperm flagellum Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134935003.

Polymorphism and mutation databases

BioMutaiTXNDC8.
DMDMi68566177.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 127127Thioredoxin domain-containing protein 8PRO_0000120162Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 35Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ6A555.
PRIDEiQ6A555.

PTM databases

PhosphoSiteiQ6A555.

Expressioni

Tissue specificityi

Testis-specific. Only expressed during spermiogenesis, prominently in the Golgi apparatus of pachytene spermatocytes and round and elongated spermatids, with a transient localization in the developing acrosome of round spermatids (at protein level).1 Publication

Gene expression databases

BgeeiQ6A555.
CleanExiHS_TXNDC8.
ExpressionAtlasiQ6A555. baseline and differential.
GenevisibleiQ6A555. HS.

Organism-specific databases

HPAiCAB024589.
HPA046844.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000363634.

Structurei

3D structure databases

ProteinModelPortaliQ6A555.
SMRiQ6A555. Positions 1-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9292ThioredoxinAdd
BLAST

Sequence similaritiesi

Belongs to the thioredoxin family.Curated
Contains 1 thioredoxin domain.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG0526.
GeneTreeiENSGT00530000063008.
HOGENOMiHOG000292977.
HOVERGENiHBG009243.
InParanoidiQ6A555.
OMAiCGADAKK.
OrthoDBiEOG79SF05.
PhylomeDBiQ6A555.
TreeFamiTF321403.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6A555-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVQIIKDTNE FKTFLTAAGH KLAVVQFSSK RCGPCKRMFP VFHAMSVKYQ
60 70 80 90 100
NVFFANVDVN NSPELAETCH IKTIPTFQMF KKSQKVTLFS RIKRIICCYR
110 120
SGFMSNLIFE FCGADAKKLE AKTQELM
Length:127
Mass (Da):14,575
Last modified:July 5, 2005 - v2
Checksum:i94BBF008A82CBD8F
GO
Isoform 2 (identifier: Q6A555-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-127: IFEFCGADAKKLEAKTQELM → CLADDGNE

Show »
Length:115
Mass (Da):13,138
Checksum:i6F205772109C5ECF
GO

Sequence cautioni

The sequence CAI14069.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti60 – 601N → H.
Corresponds to variant rs7041938 [ dbSNP | Ensembl ].
VAR_057353

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei108 – 12720IFEFC…TQELM → CLADDGNE in isoform 2. 2 PublicationsVSP_014333Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305830 mRNA. Translation: AAQ14501.1.
AL158158 Genomic DNA. Translation: CAI14069.1. Sequence problems.
BC130549 mRNA. Translation: AAI30550.1.
BC130551 mRNA. Translation: AAI30552.1.
CCDSiCCDS35104.1. [Q6A555-2]
RefSeqiNP_001003936.1. NM_001003936.3. [Q6A555-2]
NP_001273875.1. NM_001286946.1.
UniGeneiHs.147064.

Genome annotation databases

EnsembliENST00000374510; ENSP00000363634; ENSG00000204193. [Q6A555-2]
ENST00000374511; ENSP00000363635; ENSG00000204193. [Q6A555-1]
GeneIDi255220.
KEGGihsa:255220.
UCSCiuc004bes.3. human. [Q6A555-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305830 mRNA. Translation: AAQ14501.1.
AL158158 Genomic DNA. Translation: CAI14069.1. Sequence problems.
BC130549 mRNA. Translation: AAI30550.1.
BC130551 mRNA. Translation: AAI30552.1.
CCDSiCCDS35104.1. [Q6A555-2]
RefSeqiNP_001003936.1. NM_001003936.3. [Q6A555-2]
NP_001273875.1. NM_001286946.1.
UniGeneiHs.147064.

3D structure databases

ProteinModelPortaliQ6A555.
SMRiQ6A555. Positions 1-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000363634.

PTM databases

PhosphoSiteiQ6A555.

Polymorphism and mutation databases

BioMutaiTXNDC8.
DMDMi68566177.

Proteomic databases

PaxDbiQ6A555.
PRIDEiQ6A555.

Protocols and materials databases

DNASUi255220.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374510; ENSP00000363634; ENSG00000204193. [Q6A555-2]
ENST00000374511; ENSP00000363635; ENSG00000204193. [Q6A555-1]
GeneIDi255220.
KEGGihsa:255220.
UCSCiuc004bes.3. human. [Q6A555-2]

Organism-specific databases

CTDi255220.
GeneCardsiGC09M113065.
HGNCiHGNC:31454. TXNDC8.
HPAiCAB024589.
HPA046844.
neXtProtiNX_Q6A555.
PharmGKBiPA134935003.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0526.
GeneTreeiENSGT00530000063008.
HOGENOMiHOG000292977.
HOVERGENiHBG009243.
InParanoidiQ6A555.
OMAiCGADAKK.
OrthoDBiEOG79SF05.
PhylomeDBiQ6A555.
TreeFamiTF321403.

Miscellaneous databases

GenomeRNAii255220.
NextBioi92519.
PROiQ6A555.

Gene expression databases

BgeeiQ6A555.
CleanExiHS_TXNDC8.
ExpressionAtlasiQ6A555. baseline and differential.
GenevisibleiQ6A555. HS.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Spermatocyte/spermatid-specific thioredoxin-3, a novel Golgi apparatus-associated thioredoxin, is a specific marker of aberrant spermatogenesis."
    Jimenez A., Zu W., Rawe V.Y., Pelto-Huikko M., Flickinger C.J., Sutovsky P., Gustafsson J.-A., Oko R., Miranda-Vizuete A.
    J. Biol. Chem. 279:34971-34982(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), POSSIBLE FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lymph.

Entry informationi

Entry nameiTXND8_HUMAN
AccessioniPrimary (citable) accession number: Q6A555
Secondary accession number(s): A1L4I2, A6NDK7, Q5T934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: June 24, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Increased levels, possibly caused by overexpression, are observed in morphologically abnormal spermatozoa from infertile men, suggesting that it may be used as a marker of aberrant spermatogenesis.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.